Fatty Acid Binding Protein 6 Is Overexpressed in Colorectal Cancer

Ohmachi, Takahiro; Inoue, Hiroshi; Mimori, Koshi; Tanaka, Fumiaki; Sasaki, Atsushi; Kanda, Tatsuo; Fujii, Hiroshi; Yanaga, Katsuhiko; Mori, Masaki

doi:10.1158/1078-0432.ccr-05-2045

Cited by 57 publications

(56 citation statements)

References 22 publications

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“…More significantly, the transcript for IBABP-L is up-regulated in all stages of colorectal adenocarcinoma. In fact, we show that the up-regulation of IBABP in colorectal cancer reported in prior studies (14,15) can be attributed to the up-regulation of IBABP-L, whereas the expression of the previously defined form of IBABP is unchanged in colorectal cancer. Most significantly, IBABP-L is necessary for the survival of colon cancer cells in the presence of secondary bile acids.…”

Section: Introductionsupporting

confidence: 47%

“…The fact that IBABP-L is up-regulated in colorectal cancer forces a reinterpretation of two prior studies that reported that the shorter transcript of IBABP is up-regulated in colorectal cancer (14,15). Those studies were done without knowledge of IBABP-L so the PCR-based studies were done with primers that failed to distinguish between the two forms of IBABP.…”

Section: Discussionmentioning

confidence: 99%

“…In the first step, cDNA was synthesized from total RNA. For each sample, RNA (2.0 Ag) was reverse transcribed using SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen) in a 20 AL final reaction volume containing 10 mmol/L deoxynucleotide triphosphate mix (1.0 AL), 0.5 Ag/mL oligo(dT) [12][13][14][15][16][17][18] (in exon 7); the primer set of ARPP0 is 5 ¶-CAAGACTGGAGACAAAGTGG-3 ¶ and 5 ¶-AATCTGCAGA-CAGACACTGG-3 ¶. All primers were designed using PrimerSelect (DNAStar) and synthesized by Integrated DNA Technologies, Inc.…”

Section: Introductionmentioning

confidence: 99%

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A Novel Variant of Ileal Bile Acid Binding Protein Is Up-regulated through Nuclear Factor-κB Activation in Colorectal Adenocarcinoma

2007

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Ileal bile acid binding protein (IBABP) is the only cytosolic protein known to bind and transport bile acids. Because IBABP is reportedly up-regulated in colorectal cancer, it has been suggested as a link between bile acids and the risk of colorectal cancer. However, in this study, we show that IBABP is not up-regulated. Rather, a novel transcript of the IBABP gene, which encodes an additional 49 NH 2 -terminal amino acid residues, is up-regulated in colorectal cancer (P < 0.001). The novel transcript, called IBABP-L, is also distinct from IBABP because its transcription is controlled by nuclear factor-KB (NF-KB) rather than by the farnesoid X receptor. Most significantly, IBABP-L is necessary for the survival of HCT116 colon cancer cells in the presence of physiologic levels of the secondary bile acid deoxycholate. Collectively, the studies point toward a unique bile acid response pathway involving NF-KB and IBABP-L that could be useful for diagnosis and could potentially be targeted for therapeutic benefit.

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Section: Introductionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Novel Variant of Ileal Bile Acid Binding Protein Is Up-regulated through Nuclear Factor-κB Activation in Colorectal Adenocarcinoma

2007

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“…We had previously reported that the expression of FABP6 was higher in primary colorectal cancers and adenomas than in normal epithelium, and FABP6 might play an important role in early carcinogenesis. 34 Here, we focused on STC2 as it was one of the upregulated genes.…”

Section: Discussionmentioning

confidence: 99%

Clinicopathological significance of stanniocalcin 2 gene expression in colorectal cancer

Ieta

Tanaka

Yokobori

et al. 2009

Intl Journal of Cancer

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Laser microdissection (LMD) and microarray were used to identify genes associated with colorectal cancer. Stanniocalcin 2 (STC2) expression and clinicopathological significance in 139 clinical colorectal cancer samples were specifically investigated using real‐time quantitative reverse transcription‐polymerase chain reaction. A number of genes upregulated in colorectal cancer cells compared to normal colorectal epithelial cells were identified including STC2. STC2 gene expression in cancer tissue was higher than in corresponding normal colorectal epithelial tissue in 124 of 139 cases (89.2%, p < 0.01). Tumors with high STC2 expression showed higher frequencies of lymph node metastasis, lymphatic invasion, tumor depth, tumor size and AJCC Stage classification (p < 0.01). Patients with high STC2 expression also showed significantly worse overall survival rates than those with low STC2 expression (p < 0.01). Furthermore, STC2 gene appeared to be associated with colorectal cancer progression and may be a useful prognostic indicator for colorectal cancer. © 2009 UICC

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“…We previously identified genes that were expressed in colorectal cancer cells using laser microdissection and cDNA microarray (24,25). In the current study, to identify CT antigens more efficiently, we analyzed genes that were overexpressed in both colorectal cancer cells and normal testis using three-step microarray analysis.…”

Section: Introductionmentioning

confidence: 99%

Efficient Identification of a Novel Cancer/Testis Antigen for Immunotherapy Using Three-Step Microarray Analysis

et al. 2008

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Advanced technology in molecular biology has provided us powerful tools for the diagnosis and treatment for cancer. We herein adopted a new methodology to identify a novel cancer/ testis (CT) antigen with high frequency of expression in colorectal cancer as follows: (a) combining laser microdissection and cDNA microarray was used to analyze the gene expression profile of colorectal cancer cells; (b) genes overexpressed in testis and underexpressed in normal colon epithelium were analyzed using cDNA microarray; and (c) the gene expression profile of colorectal cancer cells was compared with that of normal testis. Using this methodology, we selected 38 candidates for CT antigen. Among these genes, we identified a novel CT antigen, serine/threonine kinase 31 (STK31), which was previously reported as a gene expressed in spermatogonia. Reverse transcription-PCR analysis showed that STK31 gene expression levels in cancer samples were significantly higher (P < 0.0001) than those in normal samples. The STK31 gene was frequently expressed not only in colorectal cancer but also in gastric and esophageal cancer. Moreover, STK31 peptide was able to elicit specific CTLs and induced CTLs lysed either peptide-loading or endogenously STK31-expressing target cells. These results showed that the new methodology in this study facilitated identification of CT antigens and that STK31 may be a candidate for cancer immunotherapy against gastrointestinal cancer. [Cancer Res 2008;68(4):1074-82]

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Fatty Acid Binding Protein 6 Is Overexpressed in Colorectal Cancer

Cited by 57 publications

References 22 publications

A Novel Variant of Ileal Bile Acid Binding Protein Is Up-regulated through Nuclear Factor-κB Activation in Colorectal Adenocarcinoma

A Novel Variant of Ileal Bile Acid Binding Protein Is Up-regulated through Nuclear Factor-κB Activation in Colorectal Adenocarcinoma

Clinicopathological significance of stanniocalcin 2 gene expression in colorectal cancer

Efficient Identification of a Novel Cancer/Testis Antigen for Immunotherapy Using Three-Step Microarray Analysis

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