Fatty acid and phospholipid composition of five psychrotrophic Pseudomonas spp. grown at different temperatures

Bhakoo, Manmohan; Herbert, R. A.

doi:10.1007/bf00421890

Cited by 53 publications

(32 citation statements)

References 14 publications

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“…Similar stability of the polar lipid composition has been described for other Gram-negative bacteria, including carbon-limited chemostat cultures of psychrotrophic pseudomonads (Bhakoo & Herbert, 1980), and also batch-grown…”

Section: Effects Of Growth Temperaturesupporting

confidence: 74%

Phenotypic variation of lipid composition in Burkholderia cepacia: a response to increased growth temperature is a greater content of 2-hydroxy acids in phosphatidylethanolamine and ornithine amide lipid

1998

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Burkho/deria cepacia produces an unusual range of polar lipids, which includes two forms each of phosphatidylethanolamine (PE) and ornithine amide lipid (OL), differing in the presence or absence of 2-hydroxy fatty acids. By using chemostat cultures in chemically defined media, variations in the lipid content and the proportions of individual lipids have been studied as a function of (a) growth temperature, (b) growth rate and (c) growth-limiting nutrient (carbon, magnesium, phosphorus or oxygen). Total cellular lipid in carbon-limited cultures was lowest at high growth temperatures and low growth rates. Increases in growth temperature over the range 2540 O C led t o increases in the proportions of molecular species of PE and OL containing 2-hydroxy acids, without changing the PE:OL ratio. Growth temperature did not alter the balance between neutral and acidic lipids, but the contribution of phosphatidylglycerol to the latter increased with rising growth temperature and growth rate. Pigmentation of cells and the presence of flagella were also temperature-dependent. Change in growth rate also affected the PE :OL ratio and the extent to which monoenoic acids were replaced by their cyclopropane derivatives. Whereas similar lipid profiles were found for carbon-, magnesiumand oxygen-limited cultures, ornithine amides were the only polar lipids detected in phosphorus-limited cells.

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Section: Effects Of Growth Temperaturesupporting

confidence: 74%

Phenotypic variation of lipid composition in Burkholderia cepacia: a response to increased growth temperature is a greater content of 2-hydroxy acids in phosphatidylethanolamine and ornithine amide lipid

1998

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“…by Bhakoo & Herbert (1980) demonstrated no significant change in fatty acid composition when the incubation temperature was reduced from 20 to 0 "C. It was suggested that the high levels of unsaturated fatty acids in these organisms grown at 20 "C (59-71 O h ) allowed sufficient membrane fluidity to be maintained for proper cellular function at the lower temperature. A similar trend was found for a psychrophilic Vibrio sp., which contained over 68 % 16: 1 when grown at 15 "C, but did not significantly alter its fatty acid composition when transferred to 0 O C (Bhakoo & Herbert, 1979).…”

Section: Discussionmentioning

confidence: 96%

Manipulation of polyunsaturated, branchedchain and trans-fatty acid production in Shewanella putrefaciens strain ACAM 342

1994

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Shewanella putrefaciens strain ACAM 342 produced a range of polyunsaturated fatty acids (PUFA) including 18:2cu6, 18:3cu3, 18:3w6, 18:40>3 and 20: 5~13 (EPA). Under culture in Zobell's broth at 15 "C exponential-phase cultures produced 3. This bacterium represents a model organism to study bacterial PUFA production. The trans-monounsaturated fatty acid 16:lw7t was also produced under all culture conditions, together with four other trans-isomers, namely 14: lw5t, 15:lco6t, 17:lw8t and 18:l(u7t. Although the relative levels of trans-acids also changed under various culture conditions, there was no evidence that they were produced as a starvation or stress response.

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“…In order to gain further insight into the possible correlations between the taxonomy, metabonomics, and ecological properties of the source organisms and the NF-B inhibitory potential of the extracts, we evaluated the FA composition of the microalgal samples included in the screening. Algae are known to be rich in FAs [70][71][72], and their FA composition can, to a certain extent, be used as fingerprints for their taxonomy or for the environmental conditions of their habitat [32,34,70]. Little difference could be seen between the FA composition of the two genera.…”

Section: Discussionmentioning

confidence: 99%

The inhibition of TNF-α-induced NF-κB activation by marine natural products

Folmer

Jaspars

Solano

et al. 2009

Biochemical Pharmacology

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, et al.. The inhibition of TNF-α induced NF-κB activation by marine natural products. Biochemical Pharmacology, Elsevier, 2009, 78 (6) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A c c e p t e d M a n u s c r i p t 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 AbstractThe deregulated activation of NF-B is associated with cancer development and inflammatory diseases. With the aim to find new NF-B inhibitors, we purified and characterized compounds from extracts of the Fijian sponge Rhabdastrella globostellata, the crinoid Comanthus parvicirrus, the soft corals Sarcophyton sp. nov. and Sinularia sp., and the gorgonian Subergorgia sp. after an initial screening of 266 extracts from different marine origins.Results obtained show that selected purified compounds had a cytotoxic effect on the human leukaemia cell line K562, inhibited both TNF--induced NF-B-DNA binding as well as TNF- induced IB degradation and nuclear translocation of p50/p65. Furthermore, we observed inhibition of NF-B activation induced by an overexpression of TNFR1, TRAF2, TRADD, or IKK Interestingly, natural products inhibited IKK kinase as well as the 26S proteasome proteolytic activity.

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Fatty acid and phospholipid composition of five psychrotrophic Pseudomonas spp. grown at different temperatures

Cited by 53 publications

References 14 publications

Phenotypic variation of lipid composition in Burkholderia cepacia: a response to increased growth temperature is a greater content of 2-hydroxy acids in phosphatidylethanolamine and ornithine amide lipid

Phenotypic variation of lipid composition in Burkholderia cepacia: a response to increased growth temperature is a greater content of 2-hydroxy acids in phosphatidylethanolamine and ornithine amide lipid

Manipulation of polyunsaturated, branchedchain and trans-fatty acid production in Shewanella putrefaciens strain ACAM 342

The inhibition of TNF-α-induced NF-κB activation by marine natural products

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