Fatty Acid and Peptide Profiles in Plasma Membrane and Membrane Rafts of PUFA Supplemented RAW264.7 Macrophages

Abstract

The eukaryotic cell membrane possesses numerous complex functions, which are essential for life. At this, the composition and the structure of the lipid bilayer are of particular importance. Polyunsaturated fatty acids may modulate the physical properties of biological membranes via alteration of membrane lipid composition affecting numerous physiological processes, e.g. in the immune system. In this systematic study we present fatty acid and peptide profiles of cell membrane and membrane rafts of murine macrophages that have been supplemented with saturated fatty acids as well as PUFAs from the n-3, the n-6 and the n-9 family. Using fatty acid composition analysis and mass spectrometry-based peptidome profiling we found that PUFAs from both the n-3 and the n-6 family have an impact on lipid and protein composition of plasma membrane and membrane rafts in a similar manner. In addition, we found a relation between the number of bis-allyl-methylene positions of the PUFA added and the unsaturation index of plasma membrane as well as membrane rafts of supplemented cells. With regard to the proposed significance of lipid microdomains for disease development and treatment our study will help to achieve a targeted dietary modulation of immune cell lipid bilayers.

Perfluorooctanoic acid exposure alters polyunsaturated fatty acid composition, induces oxidative stress and activates the AKT/AMPK pathway in mouse epididymis

“…In this paper, the significant decrease in linoleic acid might be closely related to male fertility in PFOA-exposed mice. Changes in PUFA composition affects cell membrane fluidity, harms membrane function, and may even lead to death and apoptosis within cells (Labbe et al, 1995;Garcia et al, 2011;Schumann et al, 2011). PUFAs in membranes are prone to lipid peroxidation damage based on the degree of polyunsaturation of the fatty acids, among which DHA is the most susceptible to lipid peroxidation as it has six double bonds (Hulbert et al, 2007).…”

Photocatalytic antibacterial activity of nano-TiO2 (anatase)-based thin films: Effects on Escherichia coli cells and fatty acids

“…3 c) was different due to the radical reactions associated with carbon double bond. In the C 1s spectra of oleic and linoleic acid a shoulder related to C-O bond [59,61] As stearic, oleic and linoleic acids are the main components of bacterial membranes [58,67] it can be suggested that the chemical changes that were observed in those fatty acids upon exposure to photoactivated nano-TiO 2 thin film were also taking place in E. coli cells. Thus, it is reasonable to suggest that already after short-time exposure of E. coli to photo-activated nano-TiO 2 , unsaturated fatty acids first change their chemical composition followed by mineralization whereas saturated fatty acids just mineralize.…”

Pathways of polyunsaturated fatty acid utilization: Implications for brain function in neuropsychiatric health and disease

“…N-3 PUFAs also consistently have been implicated in lipid raft regulation of immunomodulation (Ariel et al, 2005; Basiouni et al, 2012; Baumer et al, 2010; Bonilla et al, 2010; Chentouf et al, 2010; De Smedt-Peyrusse et al, 2008; Diaz et al, 2002; Geyeregger et al, 2005; Gurzell et al, 2013; Hou et al, 2012; Kim et al, 2008b; Li et al, 2005; Li et al, 2007; Li et al, 2008; Murakami et al, 2012; Ruth et al, 2009; Schumann et al, 2011; Teague et al, 2013; Van Laethem and Leo, 2002; Webb et al, 2000; Wong et al, 2009a; Zeyda et al, 2002), including a direct influence on lipid raft modulation of Toll-like receptor 4, a factor governing induction of genes that regulate inflammatory responsiveness (Wong et al, 2009a). …”