Fatty acid and lipid class composition in cutaneous mucus of Atlantic salmon,<i>Salmo salar</i>(L.)

Sprague, Matthew; Desbois, Andrew P.

doi:10.1111/are.15512

Cited by 4 publications

(7 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Docosahexaenoic acid is an ω-3 fatty acid that is extremely prevalent in fish, particularly in S. salar epidermal mucus (Sargent et al, 1999;Sprague & Desbois, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, A. salmonicida samples grown in the presence of 20:5 and 22:6 showed significant deviations from the control sample in biofilm formation, permeability and antimicrobial resistance. Docosahexaenoic acid is an ω‐3 fatty acid that is extremely prevalent in fish, particularly in S. salar epidermal mucus (Sargent et al, 1999; Sprague & Desbois, 2021). Fatty acid and lipid class composition in cutaneous mucus of Atlantic salmon, S. salar revealed a predominance of ω‐3 fatty acids compared with other PUFAs (Sprague & Desbois, 2021), which is interesting considering the robust responses of A. salmonicida observed in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Not only do some steps of fatty acid incorporation remain uncharacterized, but the prevalence and purposes of environmental fatty acid utilization amongst Gram‐negative bacteria is an emerging theme in microbiology. The fish hosts for A. salmonicida , such as S. salar , S. trutta , Coregonus clupeaformis (white fish) and Salvelinus alpinus (Arctic charr), are known to contain cellular and extracellular concentrations of PUFAs that could serve as nutrients/signalling molecules for potential microbial colonizers (Kaya & Erdem, 2009; Miller et al, 2008; Sprague & Desbois, 2021; Yang & Dick, 1994). It is also acknowledged that PUFAs may aid host innate immunity by exerting antimicrobial effects against potential pathogens (Casillas‐Vargas et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Exogenous polyunsaturated fatty acids (PUFAs) influence permeability, antimicrobial peptide resistance, biofilm formation and membrane phospholipid structure in an A‐layer and non‐A‐layer strain of Aeromonas salmonicida

Hofer

Lin

House

et al. 2022

Journal of Fish Diseases

View full text Add to dashboard Cite

Aeromonas salmonicida is a Gram‐negative bacterium that can infect a wide host range of fish populations, including salmonids and non‐salmonids as well as freshwater and marine life. Some strains of A. salmonicida cause the disease furunculosis, which can cause lethargy, intestinal inflammation, ulcers, haemorrhaging and death. The infection is spread through fish‐to‐fish contact, and the presence of infection can have devastating effects on cultivated fish populations. The purpose of this study was to explore the ability of non‐A‐layer and A‐layer A. salmonicida strains to incorporate polyunsaturated fatty acids (PUFAs) into their lipid profile and test the phenotypic effects thereof. Lipids were extracted from PUFA‐exposed cultures and analysed for lipid modification by thin‐layer chromatography and ultraperformance liquid chromatography‐mass spectrometry, showing A. salmonicida, regardless of A‐layer, capable of incorporating all seven of the PUFAs studied. Phenotypic effects were determined through the use of assays that tested for biofilm formation, membrane permeability and cyclic peptide susceptibility. Temperature‐dependent effects on biofilm formation were observed, and PUFA exposure showed significant (p < .001) increases in membrane permeability as tested by the uptake of the hydrophobic compounds crystal violet and ethidium bromide. Additionally, some PUFAs elicited modest protection and vulnerability against the membrane‐targeting cyclic peptides polymyxin B (PMB) and colistin. The diverse, strain‐specific responses to exogenous PUFAs may allude to evolved adaptive strategies that enhance survival, persistence and virulence of non‐pathogenic and pathogenic members of bacteria that oscillate between environmental and fish host niches.

show abstract

“…Docosahexaenoic acid is an ω-3 fatty acid that is extremely prevalent in fish, particularly in S. salar epidermal mucus (Sargent et al, 1999;Sprague & Desbois, 2021).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exogenous polyunsaturated fatty acids (PUFAs) influence permeability, antimicrobial peptide resistance, biofilm formation and membrane phospholipid structure in an A‐layer and non‐A‐layer strain of Aeromonas salmonicida

Hofer

Lin

House

et al. 2022

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

“…Nonetheless, the efficacy of these agents on microbial cell membranes and their antioxidant properties have been shown to inhibit the growth of microorganisms and thereby promote human health and animal health. Omega fatty acids can be deliberated as potential alternative therapeutic agents because of their antimicrobial and immunomodulatory properties ( 2 ).…”

Section: Discussionmentioning

confidence: 99%

“…The body cannot produce EFAs, so they must be provided with the diet or taken as supplements. A primary function of EFAs is the production of prostaglandins, which regulate body functions such as heart rate, blood pressure, blood clotting, fertility, conception, and play a role in immune function by regulating inflammation and encouraging the body to fight infection ( 2 ). EFAs support the cardiovascular, reproductive, immune and nervous systems.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activities of microbial essential fatty acid against foodborne pathogenic bacteria

Firoozabad¹,

Nasr²

2022

IJM

View full text Add to dashboard Cite

Background and Objectives: Human health and development have been related to dietary intake of essential fatty acids (omega 3, 6 and 9) and important for brain development, immune system function, and blood pressure regulation. Microbial essential oils are more natural and safer alternatives to synthetic preservatives. These oils have been demonstrated to have antibacterial activity within food systems and may be ideal additives to food formulations. Zygomycete fungi are well-known as good candidate for production of essential oils. Materials and Methods: Essential oils of fungi Mucor rouxii, Mucor circinelloides and Cuninghamella echinulata were extracted and fatty acids were analyzed by GC, for the first, antimicrobial activity of the fungi essential oils against foodborne pathogenic bacteria E. coli, S. aureus, B. cereus, B. subtilis, and S. enterica was examined by disc diffusion and well diffusion methods and the minimal inhibitory concentrations (MIC) of oils were determined by microtiter plate. Results: The fungi oils were exhibited the stron g antibacterial effect against Gram-positive bacteria, B. cereus, S. aureus and B. subtilis higher than Gram-negative and commercial oleic acid and linoleic acid. The MIC of the fungi oil extracts was 0.25 mg/ml for B. cereus and B. subtilis and 0.5 mg/ml about S. aureus. This research demonstrated microbial essential oils may be suitable for their antimicrobial properties in food. Conclusion: Microbial essential oil with good antibacterial activity could also be used in selected cases like foodborne disease.

show abstract

Immersion challenge model for Flavobacterium psychrophilum infection of Atlantic salmon (Salmo salar L.) fry

Macchia

Inami

Ramstad

et al. 2022

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

Flavobacterium psychrophilum is the causative agent of bacterial cold‐water disease (CWBD) and rainbow trout fry syndrome (RTFS), which affect salmonids. To better understand this pathogen and its interaction with the host during infection, including to support the development of resistant breeds and new vaccines and treatments, there is a pressing need for reliable and reproducible immersion challenge models that more closely mimic natural routes of infection. The aim of this present study was to evaluate a challenge model developed previously for rainbow trout for use in Atlantic salmon. First, preliminary challenges were conducted in Atlantic salmon (n = 120) and rainbow trout (n = 80) fry using two F. psychrophilum isolates collected from each fish species, respectively; fish had been pretreated with 200 mg/L hydrogen peroxide for 1 h. Thereafter, the main challenge was performed for just one F. psychrophilum isolate for each species (at 2 × 107 CFU/mL) but using larger cohorts (Atlantic salmon: n = 1187; rainbow trout: n = 2701). Survival in the main challenge was 81.2% in Atlantic salmon (21 days post‐challenge) and 45.3% in rainbow trout (31 days post‐challenge). Mortalities progressed similarly during the preliminary and main challenges for both species, demonstrating the reproducibility of this model. This is the first immersion challenge model of F. psychrophilum to be developed successfully for Atlantic salmon.

show abstract

Fatty acid and lipid class composition in cutaneous mucus of Atlantic salmon,Salmo salar(L.)

Cited by 4 publications

References 43 publications

Exogenous polyunsaturated fatty acids (PUFAs) influence permeability, antimicrobial peptide resistance, biofilm formation and membrane phospholipid structure in an A‐layer and non‐A‐layer strain of Aeromonas salmonicida

Exogenous polyunsaturated fatty acids (PUFAs) influence permeability, antimicrobial peptide resistance, biofilm formation and membrane phospholipid structure in an A‐layer and non‐A‐layer strain of Aeromonas salmonicida

Antimicrobial activities of microbial essential fatty acid against foodborne pathogenic bacteria

Immersion challenge model for Flavobacterium psychrophilum infection of Atlantic salmon (Salmo salar L.) fry

Contact Info

Product

Resources

About