Fatty acid and hydroxy acid adaptation in three gram‐negative hydrocarbon‐degrading bacteria in relation to carbon source

Soltani, Mohamed; Metzger, Pierre; Largeau, Claude

doi:10.1007/s11745-005-1494-x

Cited by 15 publications

(6 citation statements)

References 48 publications

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“…The changes in lipid and fatty acid composition enable the microorganism to maintain membrane functions in the face of environmental fluctuations. Several microorganism species modified their membrane lipid and fatty acid composition in order to maintain membrane integrity and fluidity within an optimal range . Numerous reports considered the outer and/or cytoplasmic membranes to be the principal site of lethal damage of desiccation.…”

Section: Introductionmentioning

confidence: 99%

Growth capacity and biochemical mechanisms involved in rhizobia tolerance to salinity and water deficit

Nouairi

Hammouda

Mhamdi

et al. 2014

J. Basic Microbiol.

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The aim of the present study was to evaluate abiotic stress tolerance of rhizobial strains belonging to Mesorhizobium, Sinorhizobium, and Rhizobium genera, as well as to investigate specie specific stress response mechanisms. Effect of NaCl and PEG on growth capacity, protein, lipid peroxydation (MDA), membrane fatty acid composition and antioxidant enzymes were investigated. Growth capacity and viability of overall rhizobia strains decreased proportionally to the increase of NaCl and PEG levels in the medium. Sinorhizobium strains appeared the most tolerant, where 4H41strain was able to grow at 800 mM NaCl and 40% PEG. On the other hand, growth of R. gallicum and M. mediterraneum was inhibited by 200 mM NaCl. The content of MDA was unchanged in Sinorhizobium strains under both stresses. For Mesorhizobium, only PEG treatment increased the content of MDA. Amount of the C19:0 cyclo fatty-acid was increased in both Sinorhizobium and Mesorhizobium tolerant strains. NaCl stress increased Superoxide dismutase (SOD) activity of overall species; especially the most tolerant strain 4H41. Both treatments increased catalase (CAT) activity in 4H41, TII7, and 835 strains. Obtained results suggest that major response of tolerant Sinorhizobium and Mesorhizobium strains to NaCl and PEG stresses is a preferential accumulation of the C19:0 cyclo fatty acid within bacterial membrane as mechanism to reduce fluidity and maintain integrity. Cell integrity and functioning is also assured by maintaining and/or increasing activity of SOD and CAT antioxidant enzymes for tolerant strains to omit structural and functional damages related to reactive oxygen species overproduced under stressful conditions.

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Section: Introductionmentioning

confidence: 99%

Growth capacity and biochemical mechanisms involved in rhizobia tolerance to salinity and water deficit

Nouairi

Hammouda

Mhamdi

et al. 2014

J. Basic Microbiol.

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“…In bacterial membranes PL play key roles in energy transduction, signal transduction, solute transport, and cell-cell recognition [9]. Many microorganisms have been shown to modify lipid composition in order to maintain membrane fluidity within an optimal range [10]. In general, perturbation of membrane fluidity by extrinsic chemical agents or other factors initiates an active response based on intrinsic chemical changes such as the modification of existing lipids and the de novo synthesis that tend to counteract the perturbation [11,12].…”

Section: Introductionmentioning

confidence: 99%

Growth Temperature and Salinity Impact Fatty Acid Composition and Degree of Unsaturation in Peanut‐Nodulating Rhizobia

Paulucci

Medeot

Dardanelli

et al. 2011

Lipids

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Growth and survival of bacteria depend on homeostasis of membrane lipids, and the capacity to adjust lipid composition to adapt to various environmental stresses. Membrane fluidity is regulated in part by the ratio of unsaturated to saturated fatty acids present in membrane lipids. Here, we studied the effects of high growth temperature and salinity (NaCl) stress, separately or in combination, on fatty acids composition and de novo synthesis in two peanut-nodulating Bradyrhizobium strains (fast-growing TAL1000 and slow-growing SEMIA6144). Both strains contained the fatty acids palmitic, stearic, and cis-vaccenic + oleic. TAL1000 also contained eicosatrienoic acid and cyclopropane fatty acid. The most striking change, in both strains, was a decreased percentage of cis-vaccenic + oleic (≥ 80% for TAL1000), and an associated increase in saturated fatty acids, under high growth temperature or combined conditions. Cyclopropane fatty acid was significantly increased in TAL1000 under the above conditions. De novo synthesis of fatty acids was shifted to the synthesis of a higher proportion of saturated fatty acids under all tested conditions, but to a lesser degree for SEMIA6144 compared to TAL1000. The major adaptive response of these rhizobial strains to increased temperature and salinity was an altered degree of fatty acid unsaturation, to maintain the normal physical state of membrane lipids.

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“…Saturated and polyunsaturated fatty acids have been reported in seawater contaminated with oil (25) and oil plumes (26) and are presumably linked to hydrocarbon degradation by native bacteria. Although both saturated and polyunsaturated fatty acids have been described as intermediates of alkane microbial degradation (47), less is known about the role of hydroxylated polyunsaturated entities. Interestingly, alkane hydroxylases from the family of cytochrome P450 enzymes have been described in hydrocarbon-degrading bacteria (48).…”

Section: Discussionmentioning

confidence: 99%

Chemical Profiling Provides Insights into the Metabolic Machinery of Hydrocarbon-Degrading Deep-Sea Microbes

et al. 2020

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Marine microbes are known to degrade hydrocarbons; however, microbes inhabiting deep-sea sediments remain largely unexplored. Previous studies into the classical pathways of marine microbial metabolism reveal diverse chemistries; however, metabolic profiling of marine microbes cultured with hydrocarbons is limited. In this study, taxonomic (amplicon sequencing) profiles of two environmental deep-sea sediments (>1,200 m deep) were obtained, along with taxonomic and metabolomic (mass spectrometry-based metabolomics) profiles of microbes harbored in deep-sea sediments cultured with hydrocarbons as the sole energy source. Samples were collected from the Gulf of México (GM) and cultured for 28 days using simple (toluene, benzene, hexadecane, and naphthalene) and complex (petroleum API 40) hydrocarbon mixtures as the sole energy sources. The sediment samples harbored diverse microbial communities predominantly classified into Woeseiaceae and Kiloniellaceae families, whereas Pseudomonadaceae and Enterobacteriaceae families prevailed after sediments were cultured with hydrocarbons. Chemical profiling of microbial metabolomes revealed diverse chemical groups belonging primarily to the lipids and lipid-like molecules superclass, as well as the organoheterocyclic compound superclass (ClassyFire annotation). Metabolomic data and prediction of functional profiles indicated an increase in aromatic and alkane degradation in samples cultured with hydrocarbons. Previously unreported metabolites, identified as intermediates in the degradation of hydrocarbons, were annotated as hydroxylated polyunsaturated fatty acids and carboxylated benzene derivatives. In summary, this study used mass spectrometry-based metabolomics coupled to chemoinformatics to demonstrate how microbes from deep-sea sediments could be cultured in the presence of hydrocarbons. This study also highlights how this experimental approach can be used to increase the understanding of hydrocarbon degradation by deep-sea sediment microbes. IMPORTANCE High-throughput technologies and emerging informatics tools have significantly advanced knowledge of hydrocarbon metabolism by marine microbes. However, research into microbes inhabiting deep-sea sediments (>1,000 m) is limited compared to those found in shallow waters. In this study, a nontargeted and nonclassical approach was used to examine the diversity of bacterial taxa and the metabolic profiles of hydrocarbon-degrading deep-sea microbes. In conclusion, this study used metabolomics and chemoinformatics to demonstrate that microbes from deep-sea sediment origin thrive in the presence of toxic and difficult-to-metabolize hydrocarbons. Notably, this study provides evidence of previously unreported metabolites and the global chemical repertoire associated with the metabolism of hydrocarbons by deep-sea microbes.

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Fatty acid and hydroxy acid adaptation in three gram‐negative hydrocarbon‐degrading bacteria in relation to carbon source

Cited by 15 publications

References 48 publications

Growth capacity and biochemical mechanisms involved in rhizobia tolerance to salinity and water deficit

Growth capacity and biochemical mechanisms involved in rhizobia tolerance to salinity and water deficit

Growth Temperature and Salinity Impact Fatty Acid Composition and Degree of Unsaturation in Peanut‐Nodulating Rhizobia

Chemical Profiling Provides Insights into the Metabolic Machinery of Hydrocarbon-Degrading Deep-Sea Microbes

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