Fatty Acid and Associated Gene Expression Analyses of Three Tree Peony Species Reveal Key Genes for α-Linolenic Acid Synthesis in Seeds

Yu, Rui; Xie, Lihang; Rahman, Mahbubur; Kilaru, Aruna; Niu, Lixin; Zhang, Yanlong

doi:10.3389/fpls.2018.00106

Cited by 38 publications

(31 citation statements)

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“…Among the developing seeds of nine wild tree peony species that were previously analyzed, seeds from P. rockii contained the highest FA content (271.82 mg/g seed DW), while P. lutea had the lowest (150.09 mg/g seed DW) [1]. In addition to ALA being the major contributor for the difference in FA content in the seeds of the two species, there is also phenotypic variation in terms of size and color (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

“…Tree peony belongs to the section Moutan DC, genus Paeonia , and family Paeoniaceae and is endemic to China. Recently, tree peony has attracted wide research interests due to the high amount of polyunsaturated fatty acids (PUFAs, >90%) in its seeds [1]. The α-linolenic acid (ALA, 18:3) content of peony seed oil (>40%) is higher than that of conventional vegetable oils derived from corn (1%), soybean (8%), and rapeseed (11%) [2].…”

Section: Introductionmentioning

confidence: 99%

“…In soybean, while three FAD3 genes contribute to ALA content in seeds [19], in flax [26] and perilla [5] it is controlled by two FAD3 genes. In tree peonies, the expression levels of putative FAD2 and FAD3 were positively correlated with ALA content suggesting their role in synthesis of ALA via PC-derived pathway [1].…”

Section: Introductionmentioning

confidence: 99%

“…Recent transcriptomics of oil-rich tissues of oil palm [27], Sacha inchi [28], peanut [29], avocado [30], perilla [31], and various oilseeds [32], including tree peony seeds [1], led to the identification of key genes and regulators of TAG biosynthesis. The seed development process of tree peonies is complex, however, and the ALA accumulation is development- and species-dependent [1]. Here, we compared the developing seed transcriptome of Paeonia rockii and Paeonia lutea , a high and low ALA accumulating species, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Transcriptome Analysis Reveals an Efficient Mechanism of α-Linolenic Acid in Tree Peony Seeds

Sun

Rahman

et al. 2018

IJMS

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Tree peony (Paeonia section Moutan DC.) species are woody oil crops with high unsaturated fatty acid content, including α-linolenic acid (ALA/18:3; >40% of the total fatty acid). Comparative transcriptome analyses were carried out to uncover the underlying mechanisms responsible for high and low ALA content in the developing seeds of P. rockii and P. lutea, respectively. Expression analysis of acyl lipid metabolism genes revealed upregulation of select genes involved in plastidial fatty acid synthesis, acyl editing, desaturation, and triacylglycerol assembly in seeds of P. rockii relative to P. lutea. Also, in association with ALA content in seeds, transcript levels for fatty acid desaturases (SAD, FAD2, and FAD3), which encode enzymes necessary for polyunsaturated fatty acid synthesis, were higher in P. rockii compared to P. lutea. Furthermore, the overexpression of PrFAD2 and PrFAD3 in Arabidopsis increased linoleic and ALA content, respectively, and modulated the final ratio 18:2/18:3 in the seed oil. In conclusion, we identified the key steps and validated the necessary desaturases that contribute to efficient ALA synthesis in a woody oil crop. Together, these results will aid to increase essential fatty acid content in seeds of tree peonies and other crops of agronomic interest.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Transcriptome Analysis Reveals an Efficient Mechanism of α-Linolenic Acid in Tree Peony Seeds

Sun

Rahman

et al. 2018

IJMS

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“…First, seed oils were subjected to methylation according to the methods described by Zhang et al [52] To reuse the dried lipids, 0.5 ml of methanol containing 5 % concentrated sulfuric acid was incubated for 1 h, and then, the mixture was placed into a 90°C water bath (Thermomix, Eppendorf, Hamburg, Germany). Subsequently, the solution was cooled to room temperature.…”

Section: Fatty Acid Analysismentioning

confidence: 99%

Phenotypic Characteristics and Fatty Acid Composition of Seeds from Different Herbaceous Peony Species Native to China

Yan¹,

Xie²,

Wang³

et al. 2019

Chemistry & Biodiversity

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Herbaceous peony has been widely cultivated in China due to its substantial ornamental and medicinal value. In the present study, the phenotypic characteristics, total fatty acid (FA) content, and nine FA compositions of herbaceous peony seeds from 14 populations belonging to six species and one subspecies were determined by normal test and gas chromatography/mass spectrometry (GC/MS). The results showed that the phenotypic characteristics of seeds varied dramatically among species. The concentrations of five major FAs in seed oils were as follows: linoleic acid (173.95–236.51 μg/mg), linolenic acid (227.82–302.71 μg/mg), oleic acid (135.32–208.81 μg/mg), stearic acid (6.52–11.7 μg/mg), and palmitic acid (30.67–47.64 μg/mg). Correlation analysis demonstrated that oleic acid had the highest partial correlation coefficient with total FAs and might be applied to develop a model of phenotypic characteristics. FAs were significantly influenced by the following environmental factors: latitude, elevation, and annual average temperature. Based on the FA levels in the seed oils, clustering analysis divided 14 populations into two clusters. It was found that the average contents of oleic acid, linoleic acid, and total FAs in cluster I (147.16 μg/mg, 200.31 μg/mg, and 671.24 μg/mg, respectively) were significantly lower than those in cluster II (196.65 μg/mg, 220.16 μg/mg, and 741.78 μg/mg, respectively). Cluster I was perfectly consistent with subsect. Foliolatae, while cluster II was in good agreement with subsect. Dissectifoliae. Therefore, the FA composition of wild herbaceous peony seed oil might be used as a chemotaxonomic marker.

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Efficacy mechanisms research progress of the active components in the characteristic woody edible oils

Xu,

Wang,

Bai

et al. 2023

Food Frontiers

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Woody edible oils are a type of vegetable oil. Woody edible oils like olive oil have greater quantities of unsaturated fatty acids (UFAs), particularly essential FAs, as well as vitamin E, phytosterols, and other nutrients that are becoming more vital in human health. As a result, finding high‐quality woody oil resource plants is critical to ensuring enough edible oil supply. As six novel woody crops, Paeonia suffruticosa, Plukenetia volubilis, Acer truncatum, Olea europaea, Camellia sinensis, and Camellia oleifera are characterized by high oil production, widespread cultivation, adaptability, and various active ingredients. The six woody crop oils contain UFAs (e.g., α‐linolenic acid, oleic acid, and linoleic acid), vitamin E, polyphenols, phytosterols, and so forth. The presence of these active ingredients confers anti‐inflammatory, antioxidant, cholesterol and lipid metabolism regulating, blood lipid lowering, immune boosting, memory improving, intestinal flora regulating, and other properties to the oils, which are beneficial to body health. This article examined in depth the seed resources, FA composition, active component kinds, active ingredient efficacy mechanism, and physiological impacts of these six novel woody crop oils. These developments lay a solid platform for further study and development of these woody oil crops.

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Fatty Acid and Associated Gene Expression Analyses of Three Tree Peony Species Reveal Key Genes for α-Linolenic Acid Synthesis in Seeds

Cited by 38 publications

References 58 publications

Comparative Transcriptome Analysis Reveals an Efficient Mechanism of α-Linolenic Acid in Tree Peony Seeds

Comparative Transcriptome Analysis Reveals an Efficient Mechanism of α-Linolenic Acid in Tree Peony Seeds

Phenotypic Characteristics and Fatty Acid Composition of Seeds from Different Herbaceous Peony Species Native to China

Efficacy mechanisms research progress of the active components in the characteristic woody edible oils

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