Fatty acid and alkane changes in willow during frost-hardening

Hietala, Timo; Hiekkala, Panu; Rosenqvist, Heikki; Laakso, Simo; Tahvanainen, Liisa; Repo, Tapani

doi:10.1016/s0031-9422(97)01083-2

Cited by 13 publications

(5 citation statements)

References 15 publications

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“…In mulberry bark cells (Yoshida 1984) and in leaves of winter rye (Lynch and Steponkus 1987) substantial changes in the FA composition of the PM occured following cold acclimation. Increases in amounts of FAs and in the unsaturation to saturated FA ratio paralleled the increase in freezing tolerance during cold acclimation in willow stem samples collected in the field (Hietala et al 1998). However, there is some controversy about the possible role of changes in fatty acid composition in cold acclimation, because no changes in FA composition of the plasma membrane during cold acclimation were detected in crown tissues of orchard grass (Yoshida and Uemura 1984), winter rye seedlings (Uemura and Yoshida 1984) or tubers of Jerusalem artichoke (Ishikawa and Yoshida 1985), and no changes in FA unsaturation were detected in needles of pine seedlings artificially cold acclimated in a greenhouse (Hellergren et al 1984).…”

Section: Seasonal Changes In H + -Atpase Activity and Fatty Acid Compmentioning

confidence: 87%

Changes in freezing tolerance, plasma membrane H+-ATPase activity and fatty acid composition in Pinus resinosa needles during cold acclimation and de-acclimation

Martz¹,

Sutinen²,

Kiviniemi³

et al. 2006

Tree Physiology

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It has previously been suggested that plasma membrane ATPase (PM H+-ATPase, EC 3.6.1.3.) is a site of incipient freezing injury because activity increases following cold acclimation and there are published data indicating that activity of PM H+-ATPase is modulated by changes in lipids associated with the enzyme. To test and extend these findings in a tree species, we analyzed PM H+-ATPase activity and the fatty acid (FA) composition of glycerolipids in purified plasma membranes (PMs) prepared by the two-phase partition method from current-year needles of adult red pine (Pinus resinosa Ait.) trees. Freezing tolerance of the needles decreased from -56 degrees C in March to -9 degrees C in May, and increased from -15 degrees C in September to -148 degrees C in January. Specific activity of vanadate-sensitive PM H+-ATPase increased more than two-fold following cold acclimation, despite a concurrent increase in protein concentration. During de-acclimation, decreases in PM H+-ATPase activity and freezing tolerance were accompanied by decreases in the proportions of oleic (18:1) and linoleic (18:2) acids and increases in the proportions of palmitic (16:0) and linolenic (18:3) acids in total glycerolipids extracted from the plasma membrane fraction. This pattern of changes in PM H+-ATPase activity and the 18:1, 18:2 and 18:3 fatty acids was reversed during cold acclimation. In the PM fractions, changes in FA unsaturation, expressed as the double bond index (1 x 18:1 + 2 x 18:2 + 3 x 18:3), were closely correlated with changes in H+-ATPase specific activity (r2 = 0.995). Changes in freezing tolerance were well correlated with DBI (r2 = 0.877) and ATPase specific activity (r2 = 0.833) in the PM fraction. Total ATPase activity in microsomal fractions also closely followed changes in freezing tolerance (r2 = 0.969). We conclude that, as in herbaceous plants, simultaneous seasonal changes in PM H+-ATPase activity and fatty acid composition occur during cold acclimation and de-acclimation in an extremely winter hardy tree species under natural conditions, lending support to the hypothesis that FA-regulated PM H+-ATPase activity is involved in the cellular response underlying cold acclimation and de-acclimation.

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Section: Seasonal Changes In H + -Atpase Activity and Fatty Acid Compmentioning

confidence: 87%

Changes in freezing tolerance, plasma membrane H+-ATPase activity and fatty acid composition in Pinus resinosa needles during cold acclimation and de-acclimation

Martz¹,

Sutinen²,

Kiviniemi³

et al. 2006

Tree Physiology

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“…Moreover, linolenic acid has been demonstrated not to be directly involved in the acquisition of freezing tolerance in the cytomembranes of the needles of the extremely hardy red pine ( Martz et al , 2006 ). In contrast, FA unsaturation paralleled the increase in freezing tolerance during cold acclimation in willow stems ( Hietala et al , 1998 ), revealing that there is controversy about the possible roles of changes in FAs, and unsaturation, in the membranes during cold acclimation. Moreover, it has been proposed that changes in FAs associated with cold acclimation might include increases in linoleic acid, and decreases in linolenic acid, as occurs in the plasma membrane phospholipids of Solanum species ( Palta et al , 1993 ).…”

Section: Discussionmentioning

confidence: 99%

Cold affects the transcription of fatty acid desaturases and oil quality in the fruit of Olea europaea L. genotypes with different cold hardiness

Matteucci

D’Angeli

Errico

et al. 2011

Journal of Experimental Botany

120

118

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The olive tree lacks dormancy and is low temperature sensitive, with differences in cold tolerance and oil quality among genotypes. The oil is produced in the drupe, and the unsaturated fatty acids contribute to its quality. The aim of the present research was to investigate the relationship among development, cold response, expression of fatty acid desaturase (FAD) genes, and unsaturated fatty acid composition in drupes belonging to genotypes differing in leaf cold tolerance, but producing good oil (i.e. the non-hardy Moraiolo, the semi-hardy Frantoio, and the hardy Canino). In all genotypes, cold sensitivity, evaluated by cold-induced transient increases in cytosolic calcium, was high in the epi-mesocarp cells before oil body formation, and decreased during oil biogenesis. However, genotype-dependent differences in cold sensitivity appeared at the end of oil production. Genotype-dependent differences in FAD2.1, FAD2.2, FAD6, and FAD7 expression levels occurred in the epi-mesocarp cells during the oleogenic period. However, FAD2.1 and FAD7 were always the highest in the first part of this period. FAD2.2 and FAD7 increased after cold applications during oleogenesis, independently of the genotype. Unsaturated fatty acids increased in the drupes of the non-hardy genotype, but not in those of the hardy one, after cold exposure at the time of the highest FAD transcription. The results show a direct relationship between FAD expression and lipid desaturation in the drupes of the cold-sensitive genotype, and an inverse relationship in those of the cold-resistant genotype, suggesting that drupe cold acclimation requires a fine FAD post-transcriptional regulation. Hypotheses relating FAD desaturation to storage and membrane lipids, and genotype cold hardiness are discussed.

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“…Other important factors of frost tolerance are the concentration of polar lipids, such as an increase in phospholipids and the state of the cytoplasm (Hietala et al 1998;Yoshida 1984).…”

Section: Frost Hardening Capabilitymentioning

confidence: 99%

“…The traditional ways, such as vital staining, ethylene or ethane production- (Chalker-Scott et al 1989), phenolic-and electrolyte leakage measurements (Anisko and Lindstrom 1995) are either difficult, or expensive, requiring more practical techniques to substitute controlled freeze testing in the future (Repo et al 1997). Frost results in both quantitative and qualitative changes in cell membranes and cell water-status is the reason why frost hardiness investigations have been done by the method of electrical impedance (Hietala et al 1998). The work of a Finnish team has been principally focused on the aspects of cold acclimation and frost hardening capability of different species, such as English ryegrass (Lolium perenne) (Repo and Pulli 1996), Azalea (Rhododendron) (Väinölä and Repo 2000), Basket willow (Salix viminalis) (Repo et al 1997), Scots pine (Pinus sylvestris L.) (Repo et al 1994), and Silver birch (Betula pendula) (Luoranen et al 2004) (Table 3).…”

Section: Frost Hardening Capabilitymentioning

confidence: 99%

“…The two main types of Azalea species, i.e. the smaller leaved lepidotes with thinner cuticle, more spongy parenchyma and apoplastic space, and the group of the larger leaved lepidotes with thick cuticule and palisade parenchyma were distinguishable by the same structural principles, Basket willow (Salix viminalis L.) Repo et al (1997) Birdsfoot trefoil (Lotus corniculatus L.) Stout (1988a, b) English ryegrass (Lolium perenne L.) Repo and Pulli (1996) Scots pine (Pinus sylvestris L.) Repo et al (1994Repo et al ( , 2008 Silver birch (Betula pendula L.) Luoranen et al (2004) Willow (Salix viminalis L.) Hietala et al (1998) although the originally-studied cold hardiness evaluation gave positive results only in the case of lepidotes (Väinölä and Repo 2000).…”

Section: Frost Hardening Capabilitymentioning

confidence: 99%

See 1 more Smart Citation

Electrical impedance measurement on plants: a review with some insights to other fields

Jócsák

Végvári

Vozáry

2019

Theor. Exp. Plant Physiol.

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Electrical impedance spectroscopy (EIS) is a useful tool for the investigation of the structural characteristics of solid materials and also biological tissues. The structural changes in plant or animal tissues reflect the physiological state of the organism. Electrical impedance measurement seems to be applicable both for analytical and research laboratories, since once a stress or a quality trait is correlated with an impedance parameter the method is quick and safe for further analysis of great number of samples. This work attempted to explore the current state of literature in terms of the application of EIS that has already been done on animal and plant tissues, and more specifically searching for the possibility of wider future applicability in plant stress detection.

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Fatty acid and alkane changes in willow during frost-hardening

Cited by 13 publications

References 15 publications

Changes in freezing tolerance, plasma membrane H+-ATPase activity and fatty acid composition in Pinus resinosa needles during cold acclimation and de-acclimation

Changes in freezing tolerance, plasma membrane H+-ATPase activity and fatty acid composition in Pinus resinosa needles during cold acclimation and de-acclimation

Cold affects the transcription of fatty acid desaturases and oil quality in the fruit of Olea europaea L. genotypes with different cold hardiness

Electrical impedance measurement on plants: a review with some insights to other fields

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