Fattiviracin A1, a Novel Antiherpetic Agent Produced by Streptomyces microflavus Strain No. 2445. I. Taxonomy, Fermentation, Isolation, Physico-chemical Properties and Structure Elucidation.

Fattiviracins, Antiviral Antibiotics Produced by an Actinomycete

“…Further experimentation for the conversion of xylose to glucose was done by the [1-13 C] D-xylose feeding experiment. As shown in Table 4, 13 C incorporation was observed at each of the carbon numbers 25, 27, 23′, 25′, 1A, 3A, 1B and 3B, which correspond to the 1, 3-carbon of four glucose residues in the FV-8 molecule. These results may be explained by the conversion of xylose to glucose through the pentose phosphate pathway 25) , as shown in Fig.…”

“…1763 12) and fattiviracins (new antibiotics belonging to sugar-fatty acid lactone) produced by Streptomyces microflavus strain No. 2445 [13][14][15][16][17] . AH-135Y showed an antiherpetic activity of 2.1 µg/ml as EC 50 against HSV-1, and cytotoxicity of 30.0 µg/ml as IC 50 against Vero cells, and therefore the selective index (ratio of IC 50 to EC 50 ) was estimated as 14.3.…”

“…The biosynthesis of the non-sugar moiety of FV-8 was examined by feeding experiments using [1- Table 5, the 13 C NMR spectrum of labeled FV-8 indicated that the carbon atoms of the non-sugar moiety in the FV-8 molecule are derived from acetic acid, according to an expected polyketide pathway 26) .…”

“…To examine the incorporation of glucose into the FV-8 molecule, [1-13 C] D-glucose was fed into the culture medium. Labeled FV-8 was isolated and its 13 C NMR spectrum was examined to identify the carbon enrichment. As shown in Table 2, 13 C incorporation was observed at each of the carbon numbers 25, 23′, 1A and 1B, which correspond to the 1-carbon of four glucose residues in the FV-8 molecule.…”

“…Labeled FV-8 was isolated and its 13 C NMR spectrum was examined to identify the carbon enrichment. As shown in Table 2, 13 C incorporation was observed at each of the carbon numbers 25, 23′, 1A and 1B, which correspond to the 1-carbon of four glucose residues in the FV-8 molecule. The results suggest that the strain has the ability to directly incorporate glucose in the culture medium into the FV-8 molecule.…”