FaTT12-1 , a multidrug and toxin extrusion (MATE) member involved in proanthocyanidin transport in strawberry fruits

Chen, Siyu; Tang, Yueming; Hu, Yun; Wang, Yan; Sun, Bo; Wang, Xiaorong; Tang, Haoru; Chen, Qing

doi:10.1016/j.scienta.2017.12.032

Cited by 39 publications

(21 citation statements)

References 45 publications

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“…Recent research indicated that multi-drug and toxic extrusion MATE TT12 might be involved in transportation of anthocyanin across cell membranes in brown cotton, radish and strawberry (Gao et al, 2016, M’Mbone et al, 2018, Chen et al, 2018). In the present study, six MATE TT12 genes were identified in the strawberries and high ambient temperature up-regulate their genes expression, which was consistant with the increment of anthocyanidins levels (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis reveals fruit discoloration mechanisms in postharvest strawberries in response to high ambient temperature

et al. 2019

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Highlight High ambient temperature induced fruit discoloration of postharvest strawberries. High ambient temperature enhanced anthocyanin biosynthesis and accumulation. UAGT was the crucial enzymes involving anthocyanin glucosides in postharvest strawberries. High ambient temperature stimulated genes expression of laccase and peroxidase. Anthocyanin synthesis and degradation co-existed in postharvest strawberries under high ambient temperature.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis reveals fruit discoloration mechanisms in postharvest strawberries in response to high ambient temperature

et al. 2019

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“…Subsequently, Equal volumes of TRV1 and TRV2/ TRV2-G6PDH-CY cultures were mixed together. Transient overexpression and VIGS by infiltration of corresponding construct into strawberry fruits followed the procedures described by Chen et al (2018) [ 45 ]. The treated strawberries were cultured in dark for 12 h, and then subjected to 4 °C treatment.…”

Section: Methodsmentioning

confidence: 99%

Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response

Zhang

Luo

Cheng

et al. 2020

IJMS

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Glucose-6-phosphate dehydrogenase (G6PDH) plays an important role in plant stress responses. Here, five FaG6PDH sequences were obtained in strawberry, designated as FaG6PDH-CY, FaG6PDH-P1, FaG6PDH-P1.1, FaG6PDH-P2 and FaG6PDH-P0, which were divided into cytosolic (CY) and plastidic (P) isoforms based on the bioinformatic analysis. The respective FaG6PDH genes had distinct expression patterns in all tissues and at different stages of fruit development. Notably, FaG6PDH-CY was the most highly expressed gene among five FaG6PDH members, indicating it encoded the major G6PDH isoform throughout the plant. FaG6PDH positively regulated cold tolerance in strawberry. Inhibition of its activity gave rise to greater cold-induced injury in plant. The FaG6PDH-CY transcript had a significant increase under cold stress, similar to the G6PDH enzyme activity, suggesting a principal participant in response to cold stress. Further study showed that the low-temperature responsiveness (LTR) element in FaG6PDH-CY promoter can promote the gene expression when plant encountered cold stimuli. Besides, FaG6PDH-CY was involved in regulating cold-induced activation of antioxidant enzyme genes (FaSOD, FaCAT, FaAPX and FaGR) and RBOH-dependent ROS generation. The elevated FaG6PDH-CY enhanced ROS-scavenging capability of antioxidant enzymes to suppress ROS excessive accumulation and relieved the oxidative damage, eventually improving the strawberry resistance to cold stress.

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“…TRV2-CYC-B and TRV1 and TRV2 plasmids were transfected into Agrobacterium tumefaciens GV3101 competent cells by freeze-thaw. The method of Chen et al [32] and Zhai et al [31] were used for VIGS. Agrobacterium cells were spread in selective Luria Bertani (LB) solid medium containing Gentamicin (Gen, 40 mg/L), Kan (50 mg/L), and Rifampicin (Rif, 20 mg/L), then cultivated at 28 • C for 48 h-72 h. The growth of the colonies was observed.…”

Section: Agrobacterium Transformation and Injectionmentioning

confidence: 99%

“…The presence of the inserts was verified using 1 µL of bacterial solution as a template for PCR. TRV1 detection primers were created as described by Chen et al [32]. TRV2-CYC-B detection primers were the same as S1 primers.…”

Section: Agrobacterium Transformation and Injectionmentioning

confidence: 99%

Expression of a Chromoplast-Specific Lycopene β-Cyclase Gene (CYC-B) Is Implicated in Carotenoid Accumulation and Coloration in the Loquat

et al. 2019

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Carotenoids are the principal pigments in the loquat. Although the metabolic pathway of plant carotenoids has been extensively investigated, few studies have been explored the regulatory mechanisms of loquat carotenoids because knowledge of the loquat genome is incomplete. The chromoplast-specific lycopene β-cyclase gene (CYC-B) could catalyze cyclization of lycopene to β-carotene. In this study, the differential accumulation patterns of loquat with different colors were analyzed and virus-induced gene silencing (VIGS) was utilized in order to verify CYC-B gene function. Using a cloning strategy of homologous genes, a CYC-B gene orthologue was successfully identified from the loquat. At a later stage of maturation, CYC-B gene expression and carotenoids concentrations in the 'Dawuxing' variety were higher than in 'Chuannong 1-5-9 , possibly leading to the difference in pulp coloration of loquat. Interference of CYC-B gene expression in the loquat demonstrated clear visual changes. The green color in negative control fruits became yellow, while TRV2-CYC-B silenced fruits remained green. CYC-B gene expression and total carotenoid content in the pulp decreased by 32.5% and 44.1%, respectively. Furthermore, multiple key genes in the carotenoid metabolic pathway synergistically responded to downregulation of CYC-B gene expression. In summary, we provide direct evidences that CYC-B gene is involved in carotenoid accumulation and coloration in the loquat.They participate in the assembly of photosystems and perform a significant role in light capture, whereby they absorb light in a range of the blue spectrum broader than that achieved in chlorophyll. The excited carotenoids undergo excitation energy transfer to chlorophyll [8,9]. Carotenoids protect the photosynthetic apparatus from photooxidative damage caused by excess light energy through the quenching of both singlet and triplet state chlorophylls [9,10]. Carotenoids are conducive to produce scents and flavors that attract insects and animals for pollination and seed dispersal [11]. They are also significant precursors for the biosynthesis of metabolites, such as vitamin A, strigolactone, and abscisic acid [12][13][14][15][16]. Furthermore, carotenoids are thought to protect the human body from oxidative stress by removing free radicals, which can prevent cancer, cardiovascular diseases, and other chronic diseases [17][18][19].Sites for the biosynthesis and accumulation of plant carotenoids are plastids, containing chloroplasts and chromoplasts [20]. The plant carotenoid biosynthesis and cleavage pathway is shown in Supplementary Figure S1. Geranylgeranyl diphosphate (GGPP), a precursor of carotenoid metabolism, is catalyzed to produce phytoene by phytoene synthase (PSY). Phytoene is the first carotenoid molecule and undergoes desaturation and isomerization to form lycopene. This process is catalyzed by desaturases (phytoene desaturase, PDS; ζ-carotene desaturase, ZDS) and isomerases (carotene isomerase, CRTISO; 15-cis-ζ-carotene isomerase, ZISO). Cyclization of lycop...

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FaTT12-1 , a multidrug and toxin extrusion (MATE) member involved in proanthocyanidin transport in strawberry fruits

Cited by 39 publications

References 45 publications

Comparative transcriptome analysis reveals fruit discoloration mechanisms in postharvest strawberries in response to high ambient temperature

Comparative transcriptome analysis reveals fruit discoloration mechanisms in postharvest strawberries in response to high ambient temperature

Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response

Expression of a Chromoplast-Specific Lycopene β-Cyclase Gene (CYC-B) Is Implicated in Carotenoid Accumulation and Coloration in the Loquat

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