FATP1 silence inhibits the differentiation and induces the apoptosis in chicken preadipocytes

Qi, Renli; Feng, Min; Tan, Xiao; Gan, Lu; Gao, Yan; Sun, Cheng

doi:10.1007/s11033-012-2306-4

Cited by 18 publications

(23 citation statements)

References 18 publications

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“…Since previous studies have reported an antiapoptotic role for FATP1 [25], we speculated that hFATP1 might inhibit the wave of PR apoptosis that occurs during NR development. To assess our hypothesis, we used the TUNEL assay to analyze the number of apoptotic cells in the NR of transgenic and WT mice on postnatal (P) days 6, 9, and 12 (Fig 7).…”

Section: Resultsmentioning

confidence: 99%

“…We showed here that mice Bcl-2 and Bax mRNAs are upregulated and downregulated, respectively, by hFATP1 overexpression. Likewise, Qi et al [25] demonstrated that FATP1 silencing significantly increased the expression and activities of the proapoptotic factors caspase 3 and Bax. Interestingly, in Drosophila , fatp deficiency causes loss of PRs via a caspase-dependent death pathway, suggesting an important conserved function for FATP in PR survival [18].…”

Section: Discussionmentioning

confidence: 99%

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Fatty acid transport protein 1 regulates retinoid metabolism and photoreceptor development in mouse retina

et al. 2017

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In retinal pigment epithelium (RPE), RPE65 catalyzes the isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol in the visual cycle and controls the rhodopsin regeneration rate. However, the mechanisms by which these processes are regulated are still unclear. Fatty Acid Transport Protein 1 (FATP1) is involved in fatty acid uptake and lipid metabolism in a variety of cell types. FATP1 co-localizes with RPE65 in RPE and inhibits its isomerase activity in vitro. Here, we further investigated the role of FATP1 in the visual cycle using transgenic mice that overexpress human FATP1 specifically in the RPE (hFATP1TG mice). The mice displayed no delay in the kinetics of regeneration of the visual chromophore 11-cis-retinal after photobleaching and had no defects in light sensitivity. However, the total retinoid content was higher in the hFATP1TG mice than in wild type mice, and the transgenic mice also displayed an age-related accumulation (up to 40%) of all-trans-retinal and retinyl esters that was not observed in control mice. Consistent with these results, hFATP1TG mice were more susceptible to light-induced photoreceptor degeneration. hFATP1 overexpression also induced an ~3.5-fold increase in retinosome autofluorescence, as measured by two-photon microscopy. Interestingly, hFATP1TG retina contained ~25% more photoreceptor cells and ~35% longer outer segments than wild type mice, revealing a non-cell-autonomous effect of hFATP1 expressed in the RPE. These data are the first to show that FATP1-mediated fatty acid uptake in the RPE controls both retinoid metabolism in the outer retina and photoreceptor development.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fatty acid transport protein 1 regulates retinoid metabolism and photoreceptor development in mouse retina

et al. 2017

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“…To date, several studies have shown that PPAR γ and C/EBP α (Yu et al 2014), as well as FATP1 (Qi et al 2013) and Klf7 (Zhang et al 2013) regulate the differentiation of preadipocytes in the chicken. However, to our knowledge, investigations into the roles of lncRNAs in preadipocyte differentiation in the chicken have not yet been conducted.…”

Section: Discussionmentioning

confidence: 99%

“…Several genes have been identified as regulators of adipogenesis and preadipocyte differentiation in chickens, including KLF2 (Zhang et al 2014a), KLF3 (Zhang et al 2014b), and FATP1 (Qi et al 2013). Several recent studies have investigated the regulatory mechanisms of chicken adipogenesis using genome-wide analysis of mRNA (Ji et al 2012; Regassa and Kim 2015) and microRNA (Wang et al 2015).…”

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confidence: 99%

Genome-Wide Analysis of lncRNA and mRNA Expression During Differentiation of Abdominal Preadipocytes in the Chicken

Zhang

Han

et al. 2017

G3 Genes|Genomes|Genetics

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Long noncoding RNAs (lncRNAs) regulate adipogenesis and other processes associated with metabolic tissue development and function. However, little is known about the function and profile of lncRNAs during preadipocyte differentiation in the chicken (Gallus gallus). Herein, lncRNA and mRNA expression in preadipocytes at different stages of differentiation were analyzed using RNA sequencing. A total of 1,300,074,528 clean reads and 27,023 novel lncRNAs were obtained from 12 samples. The number of genes (1336 lncRNAs and 1759 mRNAs; 3095 in total) differentially expressed across various stages declined as differentiation progressed. Differentially expressed genes were found to be involved in several pathways related to preadipocyte differentiation that have been extensively studied, including glycerolipid metabolism, and the mammalian target of rapamycin, peroxisome proliferator-activated receptor, and mitogen-activated protein kinase signaling pathways. To our knowledge, some pathways are being reported for the first time, including the propanoate metabolism, fatty acid metabolism, and oxidative phosphorylation pathways. Furthermore, 3095 differentially expressed genes were clustered into eight clusters, and their expression patterns were determined through K-means clustering. Genes involved in the K2 cluster likely play important roles in preadipocyte differentiation. Six stage-specific modules related to A0 (day 0), A2 (day 2), and A6 (day 6) stages were identified, using weighted coexpression network analysis. Nine central, highly connected .genes in stage-specific modules were subsequently identified, including XLOC_068731, XLOC_022661, XLOC_045161, XLOC_070302, CHD6, LLGL1, NEURL1B, KLHL38, and ACTR6. This study provides a valuable resource for further study of chicken lncRNA and facilitates a better understanding of preadipocyte differentiation in the chicken

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“…antibodies, available and few transgenic animals. Gene silencing by siRNA are now routinely used in studies on cattle (Ma and Corl, 2012), sheep (Smith et al, 2012), chickens (Qi et al, 2013), pigs (Shi et al, 2013) and salmon (Kumari et al, 2013) using cell lines and some primary cells. However, there are difficulties introducing exogenous nucleotides, including siRNA, into certain primary cells, especially primary macrophages (reviewed by Zhang et al, 2009) and studies using siRNA to investigate macrophage genes lag behind those for other cell types.…”

Section: Introductionmentioning

confidence: 99%

Comparison of small interfering RNA (siRNA) delivery into bovine monocyte-derived macrophages by transfection and electroporation

Jensen

Anderson

Glass

2014

Veterinary Immunology and Immunopathology

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The manipulation of the RNA interference pathway using small interfering RNA (siRNA) has become the most frequently used gene silencing method. However, siRNA delivery into primary cells, especially primary macrophages, is often considered challenging. Here we report the investigation of the suitability of two methodologies: transient transfection and electroporation, to deliver siRNA targeted against the putative immunomodulatory gene Mediterranean fever (MEFV) into primary bovine monocyte-derived macrophages (bMDM). Eleven commercial transfection reagents were investigated with variable results with respect to siRNA uptake, target gene knock-down, cell toxicity and type I interferon (IFN) response induction. Three transfection reagents: Lipofectamine 2000, Lipofectamine RNAiMAX and DharmaFECT 3, were found to consistently give the best results. However, all the transfection reagents tested induced an IFN response in the absence of siRNA, which could be minimized by reducing the transfection reagent incubation period. In addition, optimized siRNA delivery into bMDM by electroporation achieved comparable levels of target gene knock-down as transient transfection, without a detectable IFN response, but with higher levels of cell toxicity. The optimized transient transfection and electroporation methodologies may provide a starting point for optimizing siRNA delivery into macrophages derived from other species or other cells considered difficult to investigate with siRNA.

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FATP1 silence inhibits the differentiation and induces the apoptosis in chicken preadipocytes

Cited by 18 publications

References 18 publications

Fatty acid transport protein 1 regulates retinoid metabolism and photoreceptor development in mouse retina

Fatty acid transport protein 1 regulates retinoid metabolism and photoreceptor development in mouse retina

Genome-Wide Analysis of lncRNA and mRNA Expression During Differentiation of Abdominal Preadipocytes in the Chicken

Comparison of small interfering RNA (siRNA) delivery into bovine monocyte-derived macrophages by transfection and electroporation

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