FATP1 Exerts Variable Effects on Adipogenic Differentiation and Proliferation in Cells Derived From Muscle and Adipose Tissue

Huang, Jieping; Guo, Duo; Zhu, Ruirui; Feng, Ye; Li, Ruirui; Yang, Xintong; Shi, Deshun

doi:10.3389/fvets.2022.904879

Cited by 11 publications

(8 citation statements)

References 43 publications

(51 reference statements)

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“…In the present study, FABP4 was signi cantly upregulated by lncFABP4 overexpression. Although FABP4 and HSL play important roles in lipolysis, they are commonly used as markers of adipogenesis due to their high expression levels in mature adipocytes [22,23]. Unsurprisingly, FABP4 and HSL expression was signi cantly upregulated in mature intramuscular adipocytes.…”

Section: Discussionmentioning

confidence: 99%

The role of the novel lncRNA lncFABP4 in modulating adipogenic differentiation in buffalo intramuscular preadipocytes

Zhu

Yang

et al. 2023

Preprint

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Intramuscular fat (IMF) is a crucial determinant of meat quality and is influenced by various regulatory factors. Despite the growing recognition of the important role of long noncoding RNAs (lncRNAs) in IMF deposition, the mechanisms underlying buffalo IMF deposition remain poorly understood. In this study, we identified and characterized a novel lncRNA, lncFABP4, which was transcribed from the antisense strand of FABP4. Moreover, lncFABP4 significantly increased intramuscular preadipocyte differentiation, as indicated by an increase in the expression of the adipogenic markers peroxisome proliferator-activated receptor gamma (PPARG), CCAAT enhancer binding protein alpha (C/EBPα), and fatty acid binding protein 4 (FABP4). Mechanistically, lncFABP4 was found to have the potential to regulate downstream gene expression by forming transcriptional complexes with the FABP4 protein. These findings contribute to a further understanding of the intricate mechanisms through which lncRNAs modulate intramuscular adipogenesis in buffaloes.

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Section: Discussionmentioning

confidence: 99%

The role of the novel lncRNA lncFABP4 in modulating adipogenic differentiation in buffalo intramuscular preadipocytes

Zhu

Yang

et al. 2023

Preprint

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“…A novel SNP c.−379 C>T was identified in the DGAT1 promoter region, which affects IMF content in muscle tissue but not backfat thickness. Generally, IMF content correlates positively with other fat depots simply because they are subjected to similar mechanisms of adipogenesis regulation (Malgwi et al, 2022), which has been the limitation in increasing IMF content, as other fat depots are usually augmented simultaneously (Huang et al, 2022). In this context, the muscle‐specific regulation of lipid deposition holds great promise for the efforts to introduce traits leading to increased IMF content in pigs in pig breeding programs.…”

Section: Discussionmentioning

confidence: 99%

Mutation c.−379 C>T in DGAT1 affects intramyocellular lipid content by altering MYOD1 binding affinity

et al. 2023

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Intramuscular fat (IMF) is one of the most important indexes of pork taste quality. Diacylglycerol acyltransferase 1 (DGAT1), belonging to the acylcoenzyme A: DGAT enzymes family, is a rate-limiting enzyme responsible for the final step of triglyceride (TG) synthesis. It is involved in TG storage in skeletal muscle; however, the underlying mechanism is not well understood. This study aimed to uncover functional mutations that can influence DGAT1 expression and consequently affect IMF deposition in pork. Two experimental groups containing individuals with high and low IMF content (6.23 ± 0.20 vs. 1.25 ± 0.05, p < 0.01) were formed from 260 Duroc × Large White × Yorkshire (D × L × Y) cross-bred pigs. A novel SNP c.−379 C>T was uncovered in the DGAT1 gene using comparative sequencing with pool DNA of high-and low-IMF groups. The IMF content of CT genotype individuals (3.19 ± 0.11%) was higher than that of CC genotype individuals (2.86 ± 0.11%) when analyzing 260 D × L × Y pigs (p < 0.05). The DGAT1 expression levels revealed a significant positive correlation with IMF content (r = 0.33, p < 0.01). Luciferase assay revealed that the DGAT1 promoter with the c.−379 T allele has a higher transcription activity than that bearing the C allele in C2C12 myoblast cells, but not in 3T3-L1 preadipocytes. Online prediction followed by chromatin immunoprecipitationpolymerase chain reaction assay confirmed that myogenic determination factor 1 (MYOD1) binds to the DGAT1 promoter with the c.−379 C allele but not the T allele. In vitro experiments demonstrated that MYOD1 represses DGAT1 transcription and lipogenesis. As a muscle-specific transcription factor, MYOD1 can inhibit the transcription of DGAT1 with the c.−379 C allele in muscle cells. However, in the absence of MYOD1 binding to the mutated DGAT1 promoter with the c.−379 T allele, DGAT1 expresses at a higher level in the muscle cells of the c.−379 T genotype, leading to more intramyocellular lipid accumulation than in the muscle cells of the c.−379 C genotype. The SNP c.−379 C>T in the promoter region of the DGAT1 gene provides a promising molecular marker for improving pork IMF content without affecting other fat depots.

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“…Regulating the uptake of saturated fatty acids into myoblasts reduces CPT1A-mediated fatty acid oxidation and thus promotes IMF deposition in chickens. [40,47,48,55] FASN, SREBP1, SCD Involving lipid synthesis [11,43,56,57]…”

Section: Fatp1mentioning

confidence: 99%

Genomic Insights into Molecular Regulation Mechanisms of Intramuscular Fat Deposition in Chicken

Cao,

Xing,

Guan

et al. 2023

Genes

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Intramuscular fat (IMF) plays an important role in the tenderness, water-holding capacity, and flavor of chicken meat, which directly affect meat quality. In recent years, regulatory mechanisms underlying IMF deposition and the development of effective molecular markers have been hot topics in poultry genetic breeding. Therefore, this review focuses on the current understanding of regulatory mechanisms underlying IMF deposition in chickens, which were identified by multiple genomic approaches, including genome-wide association studies, whole transcriptome sequencing, proteome sequencing, single-cell RNA sequencing (scRNA-seq), high-throughput chromosome conformation capture (HiC), DNA methylation sequencing, and m6A methylation sequencing. This review comprehensively and systematically describes genetic and epigenetic factors associated with IMF deposition, which provides a fundamental resource for biomarkers of IMF deposition and provides promising applications for genetic improvement of meat quality in chicken.

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FATP1 Exerts Variable Effects on Adipogenic Differentiation and Proliferation in Cells Derived From Muscle and Adipose Tissue

Cited by 11 publications

References 43 publications

The role of the novel lncRNA lncFABP4 in modulating adipogenic differentiation in buffalo intramuscular preadipocytes

The role of the novel lncRNA lncFABP4 in modulating adipogenic differentiation in buffalo intramuscular preadipocytes

Mutation c.−379 C>T in DGAT1 affects intramyocellular lipid content by altering MYOD1 binding affinity

Genomic Insights into Molecular Regulation Mechanisms of Intramuscular Fat Deposition in Chicken

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