Fatigue damage to pig erythrocytes during repeated swelling and shrinkage

Zou, Lili; Ding, Weiping; Sun, Shaoli; Tang, Fangqiong; Gao, Dayong

doi:10.1016/j.cryobiol.2015.07.001

Cited by 7 publications

(4 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One can envision a model that accounts for an accumulation of damage both from the concentration dependent exposure to CPA and from either the number of rapid osmotic shifts or the duration of time away from the cells’ isosmotic volume. That the cost should include some function of these two possibilities was recently experimentally verified by Zou et al [ 40 ], whose data suggest that repeated shrink-swell cycles cause cell leakage and a decrease in steady state volumes and a related increase in osmotic fragility.…”

Section: Discussionmentioning

confidence: 89%

Toxicity Minimized Cryoprotectant Addition and Removal Procedures for Adherent Endothelial Cells

et al. 2015

View full text Add to dashboard Cite

Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vitrification methods are often limited by the cytotoxicity of the concentrated cryoprotective agent (CPA) solutions that are required to suppress ice formation. Recently, we described a mathematical strategy for identifying minimally toxic CPA equilibration procedures based on the minimization of a toxicity cost function. Here we provide direct experimental support for the feasibility of these methods when applied to adherent endothelial cells. We first developed a concentration- and temperature-dependent toxicity cost function by exposing the cells to a range of glycerol concentrations at 21°C and 37°C, and fitting the resulting viability data to a first order cell death model. This cost function was then numerically minimized in our state constrained optimization routine to determine addition and removal procedures for 17 molal (mol/kg water) glycerol solutions. Using these predicted optimal procedures, we obtained 81% recovery after exposure to vitrification solutions, as well as successful vitrification with the relatively slow cooling and warming rates of 50°C/min and 130°C/min. In comparison, conventional multistep CPA equilibration procedures resulted in much lower cell yields of about 10%. Our results demonstrate the potential for rational design of minimally toxic vitrification procedures and pave the way for extension of our optimization approach to other adherent cell types as well as more complex systems such as tissues and organs.

show abstract

Section: Discussionmentioning

confidence: 89%

Toxicity Minimized Cryoprotectant Addition and Removal Procedures for Adherent Endothelial Cells

et al. 2015

View full text Add to dashboard Cite

show abstract

“…To make the volume difference between living and dead cells for the sorting process, the osmotic stimulus should be rapid because the volume of cells may recover after a certain time. 42 That is the reason why a micromixer was designed for the chip. To fully and rapidly mix the cell suspension and the hypertonic solution, many micromixers can be used, such as the Tesla micromixer, 43 the staggered herringbone micromixer, 44 and the fishhook-shaped micromixer.…”

Section: Discussionmentioning

confidence: 99%

“…Here, we adopted the hypertonic stimulus strategy because the hypertonic stimulus for cells is safer than the hypotonic stimulus (hypotonic stimulus causes the expansion of the cell volume; if the osmotic stimulus degree is low, the volume difference is small, whereas if the osmotic stimulus degree is high, the cells are prone to rupture and death). To make the volume difference between living and dead cells for the sorting process, the osmotic stimulus should be rapid because the volume of cells may recover after a certain time . That is the reason why a micromixer was designed for the chip.…”

Section: Discussionmentioning

confidence: 99%

On-Chip Label-Free Sorting of Living and Dead Cells

Wang,

Li,

Miao

et al. 2023

ACS Biomater. Sci. Eng.

Self Cite

View full text Add to dashboard Cite

With the emergence of various cutting-edge micromachining technologies, lab on a chip is growing rapidly, but it is always a challenge to realize the on-chip separation of living cells from cell samples without affecting cell activity and function. Herein, we report a novel on-chip label-free method for sorting living and dead cells by integrating the hypertonic stimulus and tilted-angle standing surface acoustic wave (T-SSAW) technologies. On a self-designed microfluidic chip, the hypertonic stimulus is used to distinguish cells by producing volume differences between living and dead cells, while T-SSAW is used to separate living and dead cells according to the cell volume difference. Under the optimized operation conditions, for the sample containing 50% of living human umbilical vein endothelial cells (HUVECs) and 50% of dead HUVECs treated with paraformaldehyde, the purity of living cells after the first separation can reach approximately 80%, while after the second separation, it can be as high as 93%; furthermore, the purity of living cells after separation increases with the initial proportion of living cells. In addition, the chip we designed is safe for cells and can robustly handle cell samples with different cell types or different causes of cell death. This work provides a new design of a microfluidic chip for label-free sorting of living and dead cells, greatly promoting the multi-functionality of lab on a chip.

show abstract

“…Постгипертонический шок эритроцитов (ПГШ) моделирует влияние факторов криоповреждения, которые действуют на этапе размораживания эритроцитов, а также при перенесении в кровеносное русло клеток, криоконсервированных под защитой проникающего криопротектора. При размораживании криоконсервированных эритроцитов, по мере таяния льда внеклеточная гипертоническая среда сменяется на изотоническую (Zou et al, 2015), вследствие чего развивается постгипертонический лизис клеток (ПГЛ). Модель «ПГШ» предполагает изучение чувствительности эритроцитов при их перенесении из гипертонических условий в изотонические.…”

Section: Introductionunclassified

Impact of amphiphilic compounds on post-hypertonic shock of human erythrocytes

2019

The Journal of v. N. Karazin Kharkiv National University, Series "Biology"

View full text Add to dashboard Cite

When the cryopreserved erythrocytes are thawed, with the ice melting the extracellular hypertonic medium changes to isotonic one, resulting in post-hypertonic cell lysis development. Under experimental conditions, the post-hypertonic shock of erythrocytes simulates the influence of cryodamage factors, acting at the erythrocyte thawing stage, as well as when the cells, cryopreserved under protection of penetrating cryoprotectant are transferred into bloodstream. Post-hypertonic shock of erythrocytes was carried out by transferring the cells from a hypertonic solution contained 1.65 mol/l NaCl (dehydration medium) into an isotonic one with 0.15 mol/l NaCl (rehydration medium) at 0ºС. The effect of specimens of various classes of amphiphilic compounds (anionic sodium decyl sulfate, non-ionic decyl-β,D-glucopyranoside, and cationic chlorpromazine) on the human erythrocyte sensitivity to post-hypertonic shock, was studied. Amphiphilic substances were supplemented into rehydration medium prior to cell introduction into it. It was shown that under post-hypertonic shock of erythrocytes, all the studied amphiphilic substances, when used in efficient concentrations, manifested a high anti-hemolytic activity (at the level of 70%). A comparative study of the efficiency of amphiphilic substances under post-hypertonic shock of erythrocytes showed differences in size of the plateau (the concentration range of amphiphilic compound, within the limits of which the minimum level of erythrocyte hemolysis was observed). Thus, it was found that for non-ionic decyl-β,D-glucopyranoside the plateau was 3 times more than for anionic sodium decyl sulfate and cationic chlorpromazine. The minimum efficient concentration for sodium decyl sulfate and the maximum one for decyl-β,D-glucopyranoside under post-hypertonic shock of erythrocytes were revealed. It is assumed that the revealed protective effect of amphiphilic compounds under post-hypertonic shock of erythrocytes is associated with their capability to integrate into membrane. This entails an increase in the surface area of the membrane and, therefore, the critical hemolytic volume of cell, which allows it to swell to a larger volume.

show abstract

Fatigue damage to pig erythrocytes during repeated swelling and shrinkage

Cited by 7 publications

References 42 publications

Toxicity Minimized Cryoprotectant Addition and Removal Procedures for Adherent Endothelial Cells

Toxicity Minimized Cryoprotectant Addition and Removal Procedures for Adherent Endothelial Cells

On-Chip Label-Free Sorting of Living and Dead Cells

Impact of amphiphilic compounds on post-hypertonic shock of human erythrocytes

Contact Info

Product

Resources

About