Fate of Myrosin Cells: Characterization of Monoclonal Antibodies Against Myrosinase

Bones, Atle M.; Thangstad, Ole Petter; Haugen, Øystein; Espevik, Terje

doi:10.1093/jxb/42.12.1541

Cited by 47 publications

(39 citation statements)

References 9 publications

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“…The myrosin cells can be easily distinguished from their neighbouring cells by light, electron and confocal microscopic observations (Bones and Iversen, 1985;Thangstad et al, 1990Thangstad et al, , 1991Bones et al, 1991;Thangstad et al, 2004;Kissen et al, 2009). The myrosin cells contain less lipids, a high content of endoplasmic reticulum and harbour smooth-looking protein bodies referred to as myrosin grains (Bones and Iversen, 1985;Thangstad et al, 1991).…”

Section: The Glucosinolate-myrosinase Defence Systemmentioning

confidence: 99%

“…Myrosin cells exist as scattered cells in stems, leaves, seeds, seedlings, petioles and roots. Brassica plants contain the enzyme myrosinase (β-thioglucoside glucohydrolase, thioglucosidase, EC 3.2.3.147 (formerly EC 3.2.3.1) (Bones and Slupphaug, 1989;Bones, 1990;Bones andRossiter, 1996, 2006), which is thought to be exclusively present in myrosin cells (Thangstad et al, 1990Bones et al, 1991;Höglund et al, 1991;Husebye et al, 2002;Thangstad et al, 2004;Kissen et al, 2009). In brassicas, myrosinases can be divided into three different gene families; the MA, MB and MC families (Xue et al, 1992;Chadchawan et al, 1993;Lenman et al, 1993;Thangstad et al, 1993;Falk et al, 1995).…”

Section: The Glucosinolate-myrosinase Defence Systemmentioning

confidence: 99%

See 1 more Smart Citation

Defence mechanisms of Brassicaceae: implications for plant-insect interactions and potential for integrated pest management. A review

Ahuja

Rohloff

Bones

2010

Agron. Sustain. Dev.

211

165

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-Brassica crops are grown worldwide for oil, food and feed purposes, and constitute a significant economic value due to their nutritional, medicinal, bioindustrial, biocontrol and crop rotation properties. Insect pests cause enormous yield and economic losses in Brassica crop production every year, and are a threat to global agriculture. In order to overcome these insect pests, Brassica species themselves use multiple defence mechanisms, which can be constitutive, inducible, induced, direct or indirect depending upon the insect or the degree of insect attack. Firstly, we give an overview of different Brassica species with the main focus on cultivated brassicas. Secondly, we describe insect pests that attack brassicas. Thirdly, we address multiple defence mechanisms, with the main focus on phytoalexins, sulphur, glucosinolates, the glucosinolate-myrosinase system and their breakdown products. In order to develop pest control strategies, it is important to study the chemical ecology, and insect behaviour. We review studies on oviposition regulation, multitrophic interactions involving feeding and host selection behaviour of parasitoids and predators of herbivores on brassicas. Regarding oviposition and trophic interactions, we outline insect oviposition behaviour, the importance of chemical stimulation, oviposition-deterring pheromones, glucosinolates, isothiocyanates, nitriles, and phytoalexins and their importance towards pest management. Finally, we review brassicas as cover and trap crops, and as biocontrol, biofumigant and biocidal agents against insects and pathogens. Again, we emphasise glucosinolates, their breakdown products, and plant volatile compounds as key components in these processes, which have been considered beneficial in the past and hold great prospects for the future with respect to an integrated pest management.Brassicas / insect pests / chemical ecology / trophic levels / glucosinolates / isothiocyanates / defence mechanisms / biocontrol / trap crops / integrated pest management

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Section: The Glucosinolate-myrosinase Defence Systemmentioning

confidence: 99%

Section: The Glucosinolate-myrosinase Defence Systemmentioning

confidence: 99%

Defence mechanisms of Brassicaceae: implications for plant-insect interactions and potential for integrated pest management. A review

Ahuja

Rohloff

Bones

2010

Agron. Sustain. Dev.

211

165

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“…These sulfurrich plant secondary metabolites are synthesized from a variety of protein amino acids (Met, Leu, Ile, Val, Trp, and Phe;Halkier and Du, 1997;Halkier and Gershenzon, 2006). The glucosinolates are part of a two-component defensive system whereby the intact glucosinolates are stored separate from their activating enzyme myrosinase (Bones et al, 1991;Bones and Rossiter, 1996;Kelly et al, 1998;Koroleva et al, 2000). Upon tissue disrup-1 These authors contributed equally to the article.tion, the glucosinolate and myrosinase combine and generate a suite of biologically active chemicals that provide resistance against lepidopteran herbivores, aphids, and pathogens (Mithen et al, 1987;Mithen, 1992;Tierens et al, 2001;Barth and Jander, 2006;Kim and Jander, 2007;de Vos et al, 2008).…”

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confidence: 99%

A Novel 2-Oxoacid-Dependent Dioxygenase Involved in the Formation of the Goiterogenic 2-Hydroxybut-3-enyl Glucosinolate and Generalist Insect Resistance in Arabidopsis

et al. 2008

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Glucosinolates are secondary metabolites found almost exclusively in the order Brassicales. They are synthesized from a variety of amino acids and can have numerous side chain modifications that control biological function. We investigated the biosynthesis of 2-hydroxybut-3-enyl glucosinolate, which has biological activities including toxicity to Caenorhabditis elegans, inhibition of seed germination, induction of goiter disease in mammals, and production of bitter flavors in Brassica vegetable crops. Arabidopsis (Arabidopsis thaliana) accessions contain three different patterns of 2-hydroxybut-3-enyl glucosinolate accumulation (present in leaves and seeds, seeds only, or absent) corresponding to three different alleles at a single locus, GSL-OH. Fine-scale mapping of the GSL-OH locus identified a 2-oxoacid-dependent dioxygenase encoded by At2g25450 required for the formation of both 2R-and 2S-2-hydroxybut-3-enyl glucosinolate from the precursor 3-butenyl glucosinolate precursor. Naturally occurring null mutations and T-DNA insertional mutations in At2g25450 exhibit a complete absence of 2-hydroxybut-3-enyl glucosinolate accumulation. Analysis of herbivory by the generalist lepidopteran Trichoplusia ni showed that production of 2-hydroxybut-3-enyl glucosinolate provides increased resistance. These results show that At2g25450 is necessary for the hydroxylation of but-3-enyl glucosinolate to 2-hydroxybut-3-enyl glucosinolate in planta and that this metabolite increases resistance to generalist herbivory.

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“…Similar to myrosinases in MIs, glucosinolates are especially abundant in sulfur-rich idioblasts termed S-cells (Bones et al, 1991). Although recent studies suggest that both myrosinases and glucosinolates are stored in separate subcellular compartments in the same cells (Koroleva and Cramer, 2011), S-cells can form groups or clusters close to MIs, and S-cells can also be located between the endodermis and phloem of each vascular bundle (Supplemental Figure 15; Kissen et al, 2009).…”

Section: Evolutionary Significance Of Myrosin Cells and Glucosinolatementioning

confidence: 99%

“…TGG4 and TGG5 encode functional myrosinases and appear to be expressed specifically in roots, while TGG6 shows expression only in pollen and does not appear to harbor myrosinase activity (Andréasson et al, 2001;Kissen et al, 2009). In Brassica napus and Sinapis alba seeds, myrosinase is found in myrosin cells in the form of watersoluble myrosin grains located in protein storage bodies in cotyledons and in the embryonic axis (Bones et al, 1991). Plant myrosinases and glucosinolates are synthesized and stored separately in adjacent cells termed myrosin cells and S-cells, respectively (Eriksson et al, 2002;Kissen et al, 2009;Ahuja et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Myrosin Idioblast Cell Fate and Development Are Regulated by theArabidopsisTranscription Factor FAMA, the Auxin Pathway, and Vesicular Trafficking

Sack

2014

The Plant Cell

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Crucifer shoots harbor a glucosinolate-myrosinase system that defends against insect predation. Arabidopsis thaliana myrosinase (thioglucoside glucohydrolase [TGG]) accumulates in stomata and in myrosin idioblasts (MIs). This work reports that the basic helix-loop-helix transcription factor FAMA that is key to stomatal development is also expressed in MIs. The loss of FAMA function abolishes MI fate as well as the expression of the myrosinase genes TGG1 and TGG2. MI cells have previously been reported to be located in the phloem. Instead, we found that MIs arise from the ground meristem rather than provascular tissues and thus are not homologous with phloem. Moreover, MI patterning and morphogenesis are abnormal when the function of the ARF-GEF gene GNOM is lost as well as when auxin efflux and vesicular trafficking are chemically disrupted. Stomata and MI cells constitute part of a wider system that reduces plant predation, the so-called "mustard oil bomb," in which vacuole breakage in cells harboring myrosinase and glucosinolate yields a brew toxic to many animals, especially insects. This identification of the gene that confers the fate of MIs, as well as stomata, might facilitate the development of strategies for engineering crops to mitigate predation.

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Fate of Myrosin Cells: Characterization of Monoclonal Antibodies Against Myrosinase

Cited by 47 publications

References 9 publications

Defence mechanisms of Brassicaceae: implications for plant-insect interactions and potential for integrated pest management. A review

Defence mechanisms of Brassicaceae: implications for plant-insect interactions and potential for integrated pest management. A review

A Novel 2-Oxoacid-Dependent Dioxygenase Involved in the Formation of the Goiterogenic 2-Hydroxybut-3-enyl Glucosinolate and Generalist Insect Resistance in Arabidopsis

Myrosin Idioblast Cell Fate and Development Are Regulated by theArabidopsisTranscription Factor FAMA, the Auxin Pathway, and Vesicular Trafficking

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