Fate of chemicals in skin after dermal application: does the in vitro skin reservoir affect the estimate of systemic absorption?

Yourick, Jeffrey J.; Koenig, Michael L.; Yourick, Debra L.; Bronaugh, Robert L.

doi:10.1016/j.taap.2003.07.015

Cited by 62 publications

(38 citation statements)

References 14 publications

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“…Similar results have also been reported using pig skin in vitro (Hawkins and Reifenrath, 1986). From these data it is clear that the HuSki model overcomes the known limitation of in vitro skin models to study the fate of lipophilic compounds in the different skin layers after topical application (Yourick et al, 2004).…”

Section: Discussionsupporting

confidence: 83%

Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo

Boudry

Blanck

Cruz

et al. 2008

J of Applied Toxicology

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This study determined and compared the percutaneous penetration and absorption of an organophosphorus (OP) pesticide, parathion (PA), using three experimental skin models: namely the human abdominal- and pig-ear skin in vitro models and the Human Skin grafted onto a nude mouse (HuSki) in vivo model. The percentage of topically applied dose absorbed and the doses present in the stratum corneum and skin were systematically determined at 24 h under similar experimental conditions. The three experimental skin models were first compared. Then, the advantages of the HuSki model for in vivo PA skin absorption studies were evaluated compared with the pig in vivo model previously used by others. Lastly, the relevance of each skin model to predict the permeability of human skin to PA in vivo was assessed by comparing our results with previously published in vivo human volunteer values. It was demonstrated that (a) pig-ear skin is relevant for predicting the in vitro human abdominal skin absorption taking into account a 2-3 times higher skin permeability to PA, (b) using ethanol as the vehicle, the absorption of PA was 4-5 times higher in the HuSki model than in the pig model but supports the usefulness of the HuSki model to easy mass balance studies, (c) both human in vitro and HuSki models closely predict the in vivo human volunteer absorption at 24 h when acetone is used as a vehicle but the HuSki model overcomes the known limitations of in vitro models for studying the fate of PA in the different skin layers after topical application.

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Section: Discussionsupporting

confidence: 83%

Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo

Boudry

Blanck

Cruz

et al. 2008

J of Applied Toxicology

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show abstract

“…stratum corneum desquamation). A number of investigators have dealt with this phenomenon with regard to assessing the risk of chemicals more accurately (Reddy et al 2000;Jung et al 2003;Yourick et al 2004). From an occupational point of view exposure to pesticides occurs frequently and, in certain periods, more or less on a daily basis; thus, repeated exposure to a substance is common practice.…”

Section: Discussionmentioning

confidence: 99%

Dermal absorption of chlorpyrifos in human volunteers

Meuling

Ravensberg

Roza

et al. 2004

Int Arch Occup Environ Health

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“…Hence, we compared VXeq skin absorption using three different receptor fluids (HBSS, HBSS-BSA 5% and HBSS-EtOH 50%). Congruent with published methods (Wilkinson et al, 2006;Yourick et al, 2004;Cross et al, 2003;Diembeck et al, 1999;Collier and Bronaugh, 1991;Brown and Ulsamer, 1975) BSA was added to improve, if any, VX solubility reaching the receptor fluid. The addition of 50% EtOH enabled smooth VX partitioning from the dermis to receptor fluid (OECD, 2004;Scott et al, 1993;Jones et al, 1989) and reproduced the receptor fluid composition used in a previous published skin permeation study with VX (Dalton et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Percutaneous penetration and distribution of VX using in vitro pig or human excised skin

et al. 2008

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Fate of chemicals in skin after dermal application: does the in vitro skin reservoir affect the estimate of systemic absorption?

Cited by 62 publications

References 14 publications

Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo

Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo

Dermal absorption of chlorpyrifos in human volunteers

Percutaneous penetration and distribution of VX using in vitro pig or human excised skin

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