Fate of 45Ca-labeled calcium hydroxide in a root canal filling paste embedded in rat subcutaneous tissues

Kawakami, Toshiyuki; Nakamura, Chihito; Hasegawa, Hiromasa; Eda, Shigeo

doi:10.1016/s0099-2399(87)80094-8

Cited by 18 publications

(14 citation statements)

References 6 publications

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“…Histopathological studies reported no damage or tissue reaction from calcium hydroxide-based root canal filling materials [4][5][6] . Kawakami et al did histopathological studies regarding tissue reactions to calcium hydroxide-based root canal filling material [5][6][7][8] . We also previously reported and discussed in detail the tissue damage and its fate in vivo [9][10][11][12] .…”

Section: Discussionmentioning

confidence: 99%

“…Histological studies of tissue reaction have also been reported [1][2][3][4][5][6] . However, most of the studies focused on the formation of localized calcification associated with the components of the material [7][8][9] . In this study, the mouse subcutaneous tissue reaction to the embedded calcium hydroxide-based root canal filling material was analyzed histologically.…”

Section: Introductionmentioning

confidence: 99%

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Mouse Subcutaneous Tissue Reaction to Calcium Hydroxide-based Root Canal Filling Material

Shoumura

Matsuda

Osuga

et al. 2014

J. Hard Tissue Biology.

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Mouse subcutaneous tissue reaction to an embedded calcium hydroxide-based root canal filling material was analyzed histopathologically. After the material was placed within the mouse dorsal subcutaneous tissues, we performed examinations using histopathological, histochemical and immunohistochemical techniques. Two weeks after embedment, the proliferation of granulation tissue had already begun to surround the calcification. Most of the cells observed were macrophages. Likewise, multinucleated giant cells increased significantly. The multinucleated giant cells were observed as two types. In one, the centers of the giant cells were vacuoles, while in the others there were deeply stained calcifications with hematoxylin. Twelve weeks after embedment of the materials, further growth of multinucleated giants cells were sighted. Histochemically, von Kossa-stainpositive granules were observed within the macrophages and multinucleated giant cells as black fine granules. According to the TRAP stained specimens, the multinucleated giant cells especially reacted strongly at 4 weeks. However, the reaction became very weak at 12 weeks. CD68 immunohistochemical staining showed positive reactions in the cytoplasm of the proliferating macrophages and multinucleated giant cells. These results suggest that multinucleated giant cells are present in the surrounding tissues due to implantation of the calcium hydroxidebased root canal filling material, and that the presence of ACP in the cells is due to ingested calcium during active phagocytosis, which would disappear later on.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mouse Subcutaneous Tissue Reaction to Calcium Hydroxide-based Root Canal Filling Material

Shoumura

Matsuda

Osuga

et al. 2014

J. Hard Tissue Biology.

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“…Bu olay özellikle vital amputasyon ve direkt kuafaj gibi vital pulpa tedavilerinde izlenen iyileşme olayları sırasında görülmektedir. [25][26][27][28] Kalsiyum hidroksit dentin dokusu üzerine uygulandığında Ca ve OH iyonlarına ayrışarak ortamın pH'ını yükseltir ve antimikrobiyal etki gösterir. Oluşan alkalen ortamda, fosfotaz enzimi kandan inorganik fosfat salınımını aktifleştirir ve kalsiyum-fosfat çökel-mesine yol açar.…”

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Vi̇tal Pulpa Tedavi̇leri̇nde Güncel Yaklaşimlar

Gökçek¹,

Bodrumlu²

2015

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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ÖZDiş çürükleri pulpal inflamasyona yol açabilen ve ileri dönemlerde diş kaybıyla sonuçlanabilen yaygın bir sağlık problemidir. İnflamasyon nedeniyle pulpa dokusu geri dönüşümlü olarak etkilendiğinde; indirekt pulpa kaplaması, direkt pulpa kaplaması, parsiyel/total pulpatomi gibi vital pulpa tedavi yöntemleri uygulanarak pulpanın canlılığının ve fonksiyonunun devam ettirilmesi sağlanabilmektedir. Dişlerin gelişim süreci boyunca meydana gelen moleküler ve histolojik değişimlerin anlaşılması ile birlikte, son yıllarda gözlenen yenilikler vital pulpa tedavilerinde yeni yaklaşımları gündeme getirmiştir Bu derlemenin amacı; dişlerin vitalitesini korumak için geçmişten günümüze kadar uygulanan vital pulpa tedavi yöntemlerinin ve kullanılan kaplama ajanlarının pulpa dokusu üzerindeki etkilerini değerlendirmektir. Anahtar Kelimeler: Vital pulpa tedavileri, kalsiyum hidroksit, MTA, Biodentin. GİRİŞPulpa, dişin kron ve kök kısmında bulunan, dentin ile çevrelenen, şekil verici, besleyici, duyusal ve koruyucu işlevlere sahip, ektomezenşim kökenli damarlanmış ve sinir ağıyla sarılmış bir bağ dokusudur. 1 Pulpa dokusunun inflamasyonu sonucunda oluşan vazodilatasyon ve artan damar permeabilitesi, pulpal doku basıncının artmasına yol açarak dokunun lokal feed-back mekanizmasını harekete geçirmek-tedir.2 Feed-back mekanizmasına göre inflame pulpada artan interstisyel basınç transkapiller hidrostatik basınç farkını düşürür ve filtrasyona engel olur. Ayrıca lokal ABSTRACTDental caries is a common problem that can cause pulpal inflammation and resultant tooth loss. When pulp tissue is reversibly inflammed, vital pulp therapies like indirect pulp capping, direct pulp capping, partial/total pulpatomy procedures are implemented to preserve vitality and function of the pulp tissue. Recent progress in understanding the molecular and histologic changes during the tooth development is lead to designing new treatment strategies in vital pulp therapies. The aim of this review is to provide an overview of vital pulp therapy from past to present and to assess the efficiency of the pulp capping materials on the pulp tissue. Key Words: Vital pulp therapy, calcium hydroxide, MTA, Biodentine olarak artmış olan interstisyel basınç inflamasyonun görülmediği bitişik dokudaki kapillerin interstisyel sıvıyı absorbe etmesine yardımcı olur ve lenf akışını arttırır. 3Bu iki mekanizma ile doku basıncının artmasına yol açan sıvı, etkilenmiş alanının dışına çıkarılarak doku basıncı düşürülmüş olur.2 Pulpanın inflamasyon sonrası uzun süre kendini koruyabilmesi ve uygun şartlar sağlandığında inflamasyonun gerileyerek pulpal sağlığın tekrar elde edilebilmesi bu mekanizmalar ile açıklanabilmektedir. Pulpa dokusunda tamirin meydana gelebilmesi için vital pulpanın varlığı şarttır

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“…이에 대해 Fuss등 15) 은 무수치 표백치아 근관 내에 수산화칼슘을 넣고 관찰한 결과, 치근면에서 pH의 변화가 없음을 보고하여 칼슘과 수산화 이온 관련을 반박하였으며 Wang등 16) 은 pH 변화가 없는 이유를 상아질의 hydroxyapatite가 OH -완충 역할을 하기 때문이라고 설명하였다. Javalet등 17) 은 원숭이의 치근첨 형성 유도를 위하여 Ca(OH)2 (pH 11.8)와 CaCl2 (pH 4.4)를 근관 내에 채운 결과, Ca(OH)2 군에서만 경조직 형성을 관찰하여 칼슘이 아닌 수산화 이온이 경조직 형성을 유도한다고 하였으며, Kawakami 18) 는 45 Ca-labeled 수산화칼슘을 이용하여 경조 직 침착을 유도하는데 있어서 수산화칼슘 자체성분의 칼슘 이 아닌, 체내에 있는 칼슘으로부터 경조직이 침착 됨을 보 고하였다. Foster등 19) 은 수산화칼슘이 근관으로부터 치근 외면으로 확산됨을 보고하였는데 특히 근관 내 도말층을 제거한 경우 에 확산이 더 잘됨을 보고 20) 하였으며 Nerwich 21) 은 치근 상 아질의 치경부가 근단부 보다 더 빨리 확산된다고 하였는데 이 확산의 차이는 상아세관의 직경, 상아세관의 수, 용매가 침투하는 상아세관의 표면 장력의 차이로 설명하였다.…”

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A study of ionic dissociation on various calcium hydroxide pastes using molecular sieving model

Lee

2002

J Korean Acad Conserv Dent

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The purpose of this study was two-fold. First was to evaluate whether the molecular sieving model was appropriate for ionic dissociation experiment. Second was to compare the dissociation of calcium and hydroxyl ions from five types of calcium hydroxide pastes (Pure calcium hydroxide paste, DT temporary dressing Each calcium hydroxide pastes was placed into 0.65ml tube with cap and then 15% polyacrylamide gel was placed onto calcium hydroxide pastes. After the gel was hardened, the tubes were filled with tridistilled water (pH 7.14) and closed with cap. The tubes were stored in 37℃, 100% incubator. The pH reading and the concentration of calcium ions were taken at 1, 4, 7, 10, and 14 days. The brain heart infusion agar plates with S. mutans and A. actinomycetemcomitans were used for antibacterial activity test. Middle of agar plate was filled with the calcium hydroxide pastes. The plates were incubated at 37℃ and observations were made to detect the zones of inhibition. These data were evaluated statistically by use of the analysis of variance and duncan test.The results were as follows. 1. In fresh mixing state, the pH of five types of calcium hydroxide pastes were measured between 12.5 and 12.8. 2. The pH was increased in all five types of calcium hydroxide pastes compared with control group. In 14 days, Pure calcium hydroxide paste (11.45) and DT temporary dressing � (11.33) showed highest pH, followed by Metapaste � (9.49), Chidopex � (8.37) and Metapex � (7.59). 3. Calcium was higher in all five types of calcium hydroxide pastes compared with control group. In 14 days, Pure calcium hydroxide paste (137.29 mg%) and DT temporary dressing � (124.6 mg%) showed highest value, followed by Metapaste � (116.74 mg%), Chidopex � (111.84 mg%) and Metapex � (60.22 mg%). 4. The zones of bacterial inhibition were seen around all five types of calcium hydroxide pastes. Chidopex � and Metapex � groups which include iodoform were observed significantly larger zone of inhibition in A. actinomycetemcomitans compared with the other calcium hydroxide groups (p<0.05). However, Metapex � showed the least antibacterial effect on S. mutans compared with other groups (p<0.05). The molecular sieving model was found to be acceptable in dissociation experiment of hydroxyl and calcium ions when compared with the previous tooth model study. But this model was not appropriate for the antibacterial test.

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Fate of 45Ca-labeled calcium hydroxide in a root canal filling paste embedded in rat subcutaneous tissues

Cited by 18 publications

References 6 publications

Mouse Subcutaneous Tissue Reaction to Calcium Hydroxide-based Root Canal Filling Material

Mouse Subcutaneous Tissue Reaction to Calcium Hydroxide-based Root Canal Filling Material

Vi̇tal Pulpa Tedavi̇leri̇nde Güncel Yaklaşimlar

A study of ionic dissociation on various calcium hydroxide pastes using molecular sieving model

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