Fatal herpesvirus infection in a litter of puppies.

Yanagisawa, Toshihiko; Azetaka, Masayuki; Midoro, Katsuo; Takahashi, Reiji; Fujiwara, Kôsaku; Sawa, Kunihiko

doi:10.1292/jvms1939.49.519

Cited by 7 publications

(4 citation statements)

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Section: Viruses and Cellsmentioning

confidence: 99%

“…Three isolates from our laboratory, YP2, YP11, and the plaque-selected YP11 (YP11mu) [25], two isolates from other laboratories, GCH-1 and Pirene [29], and two reference strains, F-205V and Glasgow CHV2 of CHV were used in this study. All CHV strains were grown in Madin-Darby canine kidney (MDCK) cells for extraction We reported previously that canine herpesvirus (CHV) gD agglutinated canine red blood cells (RBC) and that this hemagglutination (HA) activity was inhibited by monoclonal antibodies (MAbs) against CHV gD [16,24,25].…”

Section: Viruses and Cellsmentioning

confidence: 99%

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Role of One N-linked Oligosaccharide Chain on Canine Herpesvirus gD in Its Biological Activity.

Maeda

Yokoyama

Fujita

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. The YP11mu strain of a plaque-selected canine herpesvirus (CHV) encoded a smaller molecular weight (MW) of gD than those of other strains including YP2 strain . When nucleotide sequence of the mutated gD of YP11mu strain (gD(YP11mu)) was compared with that of gDs of other CHV strains, gD(YP11mu) lacked 12 nucleotides encoding 4 amino acids, NKTI, including one predicted potential N-linked glycosylation site and no other change was found in other regions. When the gD(YP11mu) and gD of YP2 strain (gD(YP2)) expressed in COS-7 and insect (Spodoptera frugiperda; Sf9) cells were compared each other, both gDs reacted with a panel of monoclonal antibodies (MAbs) against CHV gD by indirect immunofluorescence analysis and the gD (YP11mu) in the virus penetration process remains to be further analyzed.Xuan et al. [25] reported that one plaque-selected CHV, YP11mu strain, possessed a smaller molecular weight (MW) of gD than those of other strains including YP2 strain. However its HA activity and reactivity with antibodies against CHV were similar to those of other strains. Therefore, it seems that mutation in the gD of YP11mu strain does not affect biological activities of the gD. By genetical analysis of this mutation, it is expected to obtain further information on functional region of gD.In this communication, we identified the mutated region on the gD of YP11mu strain and expressed the gD in COS-7 and insect (Spodoptera frugiperda; Sf9) cells. The CHV gD expressed in COS-7 cells specifically adsorbed canine RBC and extracts of CHV gD expressed in Sf9 cells agglutinated canine RBC. Further, antibodies raised in mice immunized with recombinant CHV gDs neutralized CHV infection in vitro. MATERIALS AND METHODS Viruses and cells:Three isolates from our laboratory, YP2, YP11, and the plaque-selected YP11 (YP11mu) [25], two isolates from other laboratories, GCH-1 and Pirene [29], and two reference strains, F-205V and Glasgow CHV2 of CHV were used in this study. All CHV strains were grown in Madin-Darby canine kidney (MDCK) cells for extraction

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Section: Viruses and Cellsmentioning

confidence: 99%

Section: Viruses and Cellsmentioning

confidence: 99%

Role of One N-linked Oligosaccharide Chain on Canine Herpesvirus gD in Its Biological Activity.

Maeda

Yokoyama

Fujita

et al. 1997

The Journal of Veterinary Medical Science

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“…CHV YP11 strain, isolated from a puppy which died with acute pneumonia [18], was used in this study after plaque-selection on Madin-Darby canine kidney (MDCK) cells. MDCK cells were grown in Eagle's minimum essential medium (Nissui, Tokyo) containing 10% tryptose phosphate broth (Difco, Detroit), 5% heat-inactivated calf serum, and antibiotics.…”

Section: Virus and Celtmentioning

confidence: 99%

Neutralizing determinants of canine herpesvirus as defined by monoclonal antibodies

Xuan

Horimoto

Limcumpao

et al. 1991

Archives of Virology

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Monoclonal antibodies (MoAbs) against canine herpesvirus (CHV) were produced to identify the immunogenic proteins of the virus carrying neutralizing determinants. A panel of 24 MoAbs showing neutralizing activities was obtained and tentatively classified into 3 different groups based on their reactivity patterns in immunoblotting analysis. Group I consisting of 10 clones was specific for 145/112 kDa; Group II of 9 clones, for 80 kDa; and Group III of 5 clones, for 41 kDa glycoproteins (gps). Complement-requirement for neutralizing activities of the MoAbs suggests that gp 145/112 and gp 80 elicit mainly complement-requiring and -enhanced neutralizing antibodies, while gp 41 elicits complement-independent ones. In addition, these MoAbs were used in ELISA additivity tests for functional and topographical mapping of epitopes in each of the CHV gp. The results indicated that antigenic reactivities of gp 145/112 and gp 80 were, respectively, localized on at least 5 and 7 overlapping epitopes. On the other hand, 4 epitopes were identified on gp 41.

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“…YPll [25] and YP8702 [16] strains of CHV and C7301 strain [15] of FHV-1, which were respectively propagated in Madin-Darby canine kidney (MDCK) and Crandell feline kidney (CRFK) cell cultures, were used throughout the present study. The procedures of VN test [8,20], HI test [16], and ELISA [20] were described previously.…”

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confidence: 99%

Glycoprotein-specific immune response in canine herpesvirus infection

Xuan

Horimoto

Limcumpao

et al. 1992

Archives of Virology

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Sera from dogs which were infected with canine herpesvirus (CHV) were analyzed for their serological reactivities against virus-specific glycoproteins (gps). By sequential immunoblot analysis using sera from experimentally infected dogs, it was found that the antibody response to gp 145/112 appeared first followed by responses to gp47 and gp80. In addition, all sera from naturally infected dogs which showed neutralizing activity to CHV reacted with gp 145/112, whereas 77% and 70% reacted with gp47 and gp80, respectively. Furthermore, some of the sera also cross-neutralized feline herpesvirus type 1 (FHV-1) and reacted with gp 143/108 of FHV-1, indicating that gp 145/112 of CHV induced cross-neutralizing antibody response to FHV-1.

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Fatal herpesvirus infection in a litter of puppies.

Cited by 7 publications

References 0 publications

Role of One N-linked Oligosaccharide Chain on Canine Herpesvirus gD in Its Biological Activity.

Role of One N-linked Oligosaccharide Chain on Canine Herpesvirus gD in Its Biological Activity.

Neutralizing determinants of canine herpesvirus as defined by monoclonal antibodies

Glycoprotein-specific immune response in canine herpesvirus infection

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