Fatal Babesia canis canis infection in a splenectomized Estonian dog

Tiškina, Valentina; Capligina, Valentina; Must, Kuelli; Berzina, Inese; Ranka, Renāte; Jokelainen, Pikka

doi:10.1186/s13028-016-0189-4

Cited by 14 publications

(12 citation statements)

References 12 publications

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“…In 2015, a previously splenectomized dog was admitted to a small animal clinic of the Estonian University of Life Sciences in Tartu, Estonia, showing signs of lethargy, a change in urine color, and lack of appetite, was diagnosed with babesiosis by blood smear microscopy [ 277 ]. The clinical status of the dog worsened within 8 h, with the onset of unresponsive seizures; therefore, with the owner’s consent, the dog was euthanized.…”

Section: Northern and Northeastern Europementioning

confidence: 99%

Babesiosis in Southeastern, Central and Northeastern Europe: An Emerging and Re-Emerging Tick-Borne Disease of Humans and Animals

Bajer

Beck

et al. 2022

Microorganisms

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There is now considerable evidence that in Europe, babesiosis is an emerging infectious disease, with some of the causative species spreading as a consequence of the increasing range of their tick vector hosts. In this review, we summarize both the historic records and recent findings on the occurrence and incidence of babesiosis in 20 European countries located in southeastern Europe (Bosnia and Herzegovina, Croatia, and Serbia), central Europe (Austria, the Czech Republic, Germany, Hungary, Luxembourg, Poland, Slovakia, Slovenia, and Switzerland), and northern and northeastern Europe (Lithuania, Latvia, Estonia, Iceland, Denmark, Finland, Sweden, and Norway), identified in humans and selected species of domesticated animals (cats, dogs, horses, and cattle). Recorded cases of human babesiosis are still rare, but their number is expected to rise in the coming years. This is because of the widespread and longer seasonal activity of Ixodes ricinus as a result of climate change and because of the more extensive use of better molecular diagnostic methods. Bovine babesiosis has a re-emerging potential because of the likely loss of herd immunity, while canine babesiosis is rapidly expanding in central and northeastern Europe, its occurrence correlating with the rapid, successful expansion of the ornate dog tick (Dermacentor reticulatus) populations in Europe. Taken together, our analysis of the available reports shows clear evidence of an increasing annual incidence of babesiosis across Europe in both humans and animals that is changing in line with similar increases in the incidence of other tick-borne diseases. This situation is of major concern, and we recommend more extensive and frequent, standardized monitoring using a “One Health” approach.

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Section: Northern and Northeastern Europementioning

confidence: 99%

Babesiosis in Southeastern, Central and Northeastern Europe: An Emerging and Re-Emerging Tick-Borne Disease of Humans and Animals

Bajer

Beck

et al. 2022

Microorganisms

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“…DNA isolation from blood was performed according to the method of Tiškina et al 9 Primary and nested PCR were performed in a final volume of 25 μl reaction mixtures containing 1 × Taq Buffer with (NH4) 2 SO 4 , 2.5 mM MgCl 2 , 200 μM dNTP mixture, 0.2 μM of each primer and 0.8 U Taq DNA polymerase (recombinant) (Thermo Fisher Scientific) and 2 μl DNA template (2 μl of products from the primary PCR were used as a template for the nested PCRs). The negative control was a PCR mix with DNA substituted by water.…”

Section: Methodsmentioning

confidence: 99%

“… Spectrophotometry was used to measure the concentration and purity of the DNA (ND-1000 UV–VIS Spectrophotometer; NanoDrop Technologies) 9 bp = base pairs …”

Section: Methodsmentioning

confidence: 99%

“…The rate variation among sites was modelled with a gamma distribution (shape parameter = 1). The robustness of the generated phylogenetic Table 1 Primers used in this study [14][15][16][17][18] Pathogen species and reference Spectrophotometry was used to measure the concentration and purity of the DNA (ND-1000 UV-VIS Spectrophotometer; NanoDrop Technologies) 9 bp = base pairs tree was evaluated by bootstrap tests. 24 Association of the taxa clustering in the bootstrap test (500 replicates) is represented by the percentage next to the branches (values >50 are displayed).…”

Section: Dna Isolation and Pcrmentioning

confidence: 99%

“…[1][2][3][4][5] Canine babesiosis and granulocytic anaplasmosis are considered endemic in the Baltic countries. [6][7][8][9] To our knowledge, no studies have been performed to evaluate the presence of vector-borne pathogens in cats in the Baltics.…”

Section: Introductionmentioning

confidence: 99%

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Haemotropic Mycoplasma species in pet cats in Latvia: a study, phylogenetic analysis and clinical case report

Berzina

Capligina

Namiņa

et al. 2021

Journal of Feline Medicine and Surgery Open Reports

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Objectives The aim of this study was to evaluate whether haemotropic Mycoplasma species are detected in pet cats in Latvia, to perform a phylogenetic analysis of the detected pathogens and to report a clinical case of feline infectious anaemia. Methods Peripheral blood samples (n = 125) from pet cats were submitted; 99 samples were adequate to test for the presence of Mycoplasma species DNA by nested PCR. A clinical case was added in the later stages of the study. Positive isolates were subjected to phylogenetic analysis. Results The prevalence of ‘ Candidatus Mycoplasma haemominutum’ was 15% (n = 15/99), that of Mycoplasma haemofelis was 5% (5/99) and that of ‘ Candidatus Mycoplasma turicensis’ was 2% (n = 2/99). Cases of coinfection included ‘ Candidatus M haemominutum’ + M haemofelis (4%; n = 4/99) and ‘ Candidatus M haemominutum’ + ‘ Candidatus M turicensis’ (1%; n = 1/99). This is the first published report of M haemofelis infection in the Baltic states. Two different ‘ Candidatus M turicensis’ isolates were discovered after phylogenetic analysis. Conclusions and relevance This report is the first of an autochthonous feline infectious anaemia case in the Baltic region. The prevalence of Mycoplasma species was similar to that in other northern European countries. Phylogenetic analysis revealed variability of the isolates; one of the ‘ Candidatus M turicensis’ genotypes was detected for the first time in Europe.

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Ameliorative effects of alpha-lipoic acid and imidocarb dipropionate on clinico-haematological changes induced by experimental Babesia canis vogeli infection in dogs

et al. 2019

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Fatal Babesia canis canis infection in a splenectomized Estonian dog

Cited by 14 publications

References 12 publications

Babesiosis in Southeastern, Central and Northeastern Europe: An Emerging and Re-Emerging Tick-Borne Disease of Humans and Animals

Babesiosis in Southeastern, Central and Northeastern Europe: An Emerging and Re-Emerging Tick-Borne Disease of Humans and Animals

Haemotropic Mycoplasma species in pet cats in Latvia: a study, phylogenetic analysis and clinical case report

Ameliorative effects of alpha-lipoic acid and imidocarb dipropionate on clinico-haematological changes induced by experimental Babesia canis vogeli infection in dogs

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