“…Caco-2BBE cells were washed with sterile phosphate-buffered saline and treated with RIPA buffer containing proteinase inhibitors (Sigma-Aldrich, St. Louis, MO, USA). Cell lysates were prepared and subjected to western blotting as previously described (Moon et al 2022 ; Um et al 2023 ) using antibodies against ChREBP (NB400-13; Novus Biologicals, Centennial, CO, USA), GLUT5 (27571-1-AP; Proteintech, Rosemont, IL, USA), GAPDH (MAB374; Millipore, St. Louis, MO, USA), and TATA-binding protein (TBP) (ab63766; Abcam, Cambridge, UK). To detect nuclear ChREBP, nuclear lysates of Caco-2BBE cells were obtained using the NE-PER™ Nuclear and Cytoplasmic Extraction Reagents Kit (78833; Thermo Fisher Scientific, Carlsbad, CA, USA), according to the manufacturer’s instructions.…”