Fat Metabolism Regulates Satiety Behavior in C. elegans

Hyun, Moonjung; Davis, Kristen; Lee, Inhwan; Kim, Jeongho; Dumur, Catherine I.; You, Young-Jai

doi:10.1038/srep24841

Cited by 31 publications

(28 citation statements)

References 44 publications

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“…Finally, we used a novel computational image detection method versus detection by eye or by quantitative software not optimized for C. elegans. Despite using different animal staging, staining methods and imaging methods, we still found a 30% overlap in previously published fat phenotypes of RNAi to NHRs and a 25% overlap in RNAi to TFs (Ashrafi et al 2003;Hyun et al 2016). Our study complements earlier findings by focusing exclusively on TFs, and using precise computational quantification when examining fat storage.…”

Section: Discussionsupporting

confidence: 63%

“…3. Data from individual animals were plotted as fat score (staining intensity over threshold, arbitrary units, AU) versus body size (number of pixels Previous studies have examined C. elegans fat storage on a genome scale (Ashrafi et al 2003) and studied specific gene families such as NHRs (Arda et al 2010;Hyun et al 2016). There are several key differences between our study and those previously published.…”

Section: Discussionmentioning

confidence: 94%

“…; Hyun et al . ). There are several key differences between our study and those previously published.…”

Section: Discussionmentioning

confidence: 97%

“…; Hyun et al . ). Our study complements earlier findings by focusing exclusively on TFs, and using precise computational quantification when examining fat storage.…”

Section: Discussionmentioning

confidence: 97%

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Many transcription factors contribute to C. elegans growth and fat storage

2017

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Reverse genetic screens by RNA interference (RNAi) in model organisms such as the nematode Caenorhabditis elegans have provided numerous insights into gene function, thereby connecting genotype to phenotype. However, genes that contribute only subtly are often missed because relatively large numbers of measurements and reliable quantification are required to overcome experimental and biological noise that may mask subtle phenotypic effects. Here, we address this challenge by focusing on two phenotypes in C. elegans: growth and fat storage. We carried out comprehensive RNAi knockdown of transcription factors (TFs), as these are known important regulators of biological processes during development and the maintenance of homeostasis. Microscopy images of TF knockdown animals stained with Oil Red O (ORO) were captured, and body size (proxy for growth) and ORO staining intensity (proxy for fat storage) were precisely quantified using a newly developed imaging tool we named IPPOME (Image Processing for Precise and Objective MEasurement). We found that a surprisingly large proportion of TFs contribute to growth and fat storage, but that most TFs have only subtle, yet significant effects. This study provides a blueprint for studies of other genes and phenotypes in C. elegans.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 94%

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Many transcription factors contribute to C. elegans growth and fat storage

2017

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“…C. elegans has been successfully reported as an animal model in research related to the characterization of metabolic pathways and storage and release of lipids [41][42][43]. It maintains in its cuticle and in its intestine a range of biosynthetic and catalytic enzymes, as well as reserves of lipids within its hypodermis and intestinal cells [1,42].…”

Section: Mammals E Caenorhabiditis Elegansmentioning

confidence: 99%

Advances in the Use of Caenorhabditis Choose in the Nutritional Study of Obesity

Gh¹,

Costa²,

Tec³

et al. 2017

J Diabetes Metab

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Obesity is characterized as a health problem of high prevalence worldwide, and to date, about two thirds of the US population is affected, generating a high financial cost for health management systems. Traditionally, the model for the study of obesity has been rodents, mainly rats and mice, and although the obesity field has progressed a lot, there has been a clear need for a cheaper and more efficient model. C. elegans is a small worm with a life expectancy of about 21 days that possesses rapid growth and feeds on non-pathogenic strains of Escherichia coli. This small nematode has about 60 to 80 percent of its genes related to human diseases, and it is now known to the scientific community that manipulating its genome can provide valuable information to define the pathophysiological aspects of obesity. C. elegans has been successfully reported as an animal model in research related to nutritional physiology, in addition to the characterization of several metabolic pathways, storage mechanisms and lipid release. In recent years, due to the advances of C. elegans in the physiological characterization of lipid metabolism, it is now possible with its use as an animal model to offer the possibility of in vivo identification of compounds that modulate fat storage.

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Effect of Momordica saponin‑ and Cyclocarya paliurus polysaccharide‐enriched beverages on oxidative stress and fat accumulation in Caenorhabditis elegans

Lin

Xiao

et al. 2020

J Sci Food Agric

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BACKGROUND: As an edible and medicinal herb in Chinese folk medicine, Cyclocarya paliurus (Batal.) Iljinskaja leaves are traditionally widely used in the treatment of metabolic disorders. The vegetable Momordica charantia L. has been consumed worldwide for thousands of years as a traditional drug due to its activities against obesity and diabetes. In view of the therapeutic value of Momordica saponins (MSs) and C. paliurus polysaccharides (CPPs), an independently developed MSs-and CPPs-containing beverage (MC) was evaluated for its efficacy in controlling oxidative stress and obesity in Caenorhabditis elegans.RESULTS: First, we found that MC could promote the nuclear localization of DAF-16 and the translation of SOD-3. Further exploring its antioxidant properties, the oxidative stress by-products reactive oxygen species, malondialdehyde, and nonesterified fatty acids were significantly inhibited in C. elegans. Moreover, damage due to diseases related to oxidative stress (age pigments and neurodegenerative diseases) was alleviated. Furthermore, fat accumulation was significantly reduced in normal and high-fat models. Finally, the lipid-lowering effects of MC might involve reductions in the size and number of lipid droplets without impairing basic physiological functions in C. elegans.CONCLUSION: These results provide promising data indicating MC as an innovative health beverage for the pharmacological management of oxidative stress and obesity.

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Fat Metabolism Regulates Satiety Behavior in C. elegans

Cited by 31 publications

References 44 publications

Many transcription factors contribute to C. elegans growth and fat storage

Many transcription factors contribute to C. elegans growth and fat storage

Advances in the Use of Caenorhabditis Choose in the Nutritional Study of Obesity

Effect of Momordica saponin‑ and Cyclocarya paliurus polysaccharide‐enriched beverages on oxidative stress and fat accumulation in Caenorhabditis elegans

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