Fat depot‐specific effects of body fat distribution and adipocyte size on intramuscular fat accumulation in Wagyu cattle

Yamada, Tomoya; Kamiya, Mitsuru; Higuchi, Mikito

doi:10.1111/asj.13449

Cited by 19 publications

(17 citation statements)

References 33 publications

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“…Studies have shown that fat deposition in backfat is related to body fat percentage, carcass cross-sectional fat area ratio, intramuscular fat, and flavor and juiciness of beef ( 5 , 6 ). In Japanese Wagyu cattle, IMF content in the longest dorsal muscle was found to be positively correlated with the percentage of subcutaneous fat and maturity in vivo ( 7 , 8 ). Therefore, the identification of key genes related to subcutaneous adipose tissue by a new method is an important strategy for carcass and meat quality genetic improvement.…”

Section: Introductionmentioning

confidence: 99%

Weighted Gene Co-Expression Network Analysis Identifies Key Modules and Central Genes Associated With Bovine Subcutaneous Adipose Tissue

et al. 2022

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BackgroundFat deposition is an important economic trait in livestock and poultry production. However, the relationship between various genes and signal pathways of fat deposition is still unclear to a large extent. The purpose of this study is to analyze the potential molecular targets and related molecular pathways in bovine subcutaneous adipose tissue.ResultsWe downloaded the GSE116775 microarray dataset from Gene Expression Omnibus (GEO). The weighted gene co-expression network (WGCNA) was used to analyze the gene expression profile, and the key gene modules with the highest correlation with subcutaneous adipose tissue were identified, and the functional enrichment of the key modules was analyzed. Then, the “real” Hub gene was screened by in-module analysis and protein–protein interaction network (PPI), and its expression level in tissue samples and adipocytes was verified. The study showed that a total of nine co-expression modules were identified, and the number of genes in these modules ranged from 101 to 1,509. Among them, the blue module is most closely related to subcutaneous adipose tissue, containing 1,387 genes. These genes were significantly enriched in 10 gene ontologies including extracellular matrix organization, biological adhesion, and collagen metabolic process, and were mainly involved in pathways including ECM-receptor interaction, focal adhesion, cAMP signaling pathway, PI3K-AKT signaling pathway, and regulation of lipolysis in adipocytes. In the PPI network and coexpression network, five genes (CAV1, ITGA5, COL5A1, ABL1, and HSPG2) were identified as “real” Hub genes. Analysis of Hub gene expression by dataset revealed that the expression of these Hub genes was significantly higher in subcutaneous adipose tissue than in other tissues. In addition, real-time fluorescence quantitative PCR (qRT-PCR) analysis based on tissue samples and adipocytes also confirmed the above results.ConclusionIn this study, five key genes related to subcutaneous adipose tissue were discovered, which laid a foundation for further study of the molecular regulation mechanism of subcutaneous adipose tissue development and adipose deposition.

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Section: Introductionmentioning

confidence: 99%

Weighted Gene Co-Expression Network Analysis Identifies Key Modules and Central Genes Associated With Bovine Subcutaneous Adipose Tissue

et al. 2022

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“…IMF can be used to store energy and functions during exercise, but an excessive accumulation of IMF in the muscle is related to many diseases in people, such as diabetes, insulin resistance, lipodystrophy, etc. ( Goodpaster et al, 2000 ; Shoelson et al, 2007 ; Tada et al, 2020 ; Yamada et al, 2020 ). The IMF content in animals is not only an important index of high quality of meat but also an important character of good germplasm.…”

Section: Introductionmentioning

confidence: 99%

miR-10a-5p Inhibits the Differentiation of Goat Intramuscular Preadipocytes by Targeting KLF8 in Goats

Wang

et al. 2021

Front. Mol. Biosci.

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Intramuscular fat contributes to the improvement of meat quality of goats. MicroRNAs (miRNAs) have been reported to regulate adipocyte differentiation and maturation. The aim of our study was to clarify whether miR-10a-5p regulates goat intramuscular preadipocyte (GIPC) differentiation and its direct downstream signaling pathway. GIPCs were isolated from longissimus dorsi, whose miR-10a-5p level was measured at different time point of differentiation induction. Adipogenic differentiation of the GIPCs was evaluated by Oil Red O and BODIPY staining, and the expression changes of adipogenic genes like ACC, ATGL, CEBPβ, PPARγ, etc. Related mechanisms were verified by qPCR, a bioinformatic analysis, a dual-luciferase reporter assay, overexpression, and siRNA transfection. Oil Red O and BODIPY staining both with adipogenic gene detection showed that miR-10a-5p suppressed the accumulation of lipid droplets in GIPCs and inhibited its differentiation. The dual-luciferase reporter assay experiment revealed that miR-10a-5p regulates GIPC differentiation by directly binding to KLF8 3’UTR to regulate its expression. Thus, the results indicated that miR-10a-5p inhibits GIPC differentiation by targeting KLF8 and supply a new target for fat deposition and meat quality improvement.

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“…In Table 1 , the longissimus muscle (LM) of Wagyu cattle contains the highest IMF content (31.8-37.8% from two publications of Irie et al. (2011) and Yamada et al. (2020) .…”

Section: Genetic Factorsmentioning

confidence: 99%

“…Numerous studies agreed that there is a positive correlation between the quantity of intramuscular adipocytes and the increased adipogenesis in fetal muscle ( Yamada et al., 2020 , Chen et al., 2019 , Park et al., 2018a ). Calves will form fewer intramuscular preadipocytes if they experience undernutrition conditions in late fetal and neonatal stages.…”

Section: Nutritional Factorsmentioning

confidence: 99%

Main regulatory factors of marbling level in beef cattle

Nguyen

Malau‐Aduli

2021

Veterinary and Animal Science

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The content of intramuscular fat (IMF), that determines marbling levels is considered as one of the vital factors influencing beef sensory quality including tenderness, juiciness, flavour and colour. The IMF formation in cattle commences around six months after conception, and continuously grows throughout the life of the animal. The accumulation of marbling is remarkably affected by genetic, sexual, nutritional and management factors. In this review, the adipogenesis and lipogenesis process regulated by various factors and genes during fetal and growing stages is briefly presented. We also discuss the findings of recent studies on the effects of breed, gene, heritability and gender on the marbling accumulation. Various research reported that feeding during pregnancy, concentrate to roughage ratios and the supplementation or restriction of vitamin A, C, and D are crucial nutritional factors affecting the formation and development of IMF. Castration and early weaning combined with high energy feeding are effective management strategies for improving the accumulation of IMF. Furthermore, age and weight at slaughter are also reviewed because they have significant effects on marbling levels. The combination of several factors could positively affect the improvement of the IMF deposition. Therefore, advanced strategies that simultaneously apply genetic, sexual, nutritional and management factors to achieve desired IMF content without detrimental impacts on feed efficiency in high-marbling beef production are essential.

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Fat depot‐specific effects of body fat distribution and adipocyte size on intramuscular fat accumulation in Wagyu cattle

Abstract: In beef cattle, the accumulation of intramuscular adipose tissue within longissimus muscle, called marbling, is important for determining the quality grade of beef. Japanese black cattle (Wagyu) are characterized by the ability to accumulate high amounts of intramus

Cited by 19 publications

References 33 publications

Weighted Gene Co-Expression Network Analysis Identifies Key Modules and Central Genes Associated With Bovine Subcutaneous Adipose Tissue

Weighted Gene Co-Expression Network Analysis Identifies Key Modules and Central Genes Associated With Bovine Subcutaneous Adipose Tissue

miR-10a-5p Inhibits the Differentiation of Goat Intramuscular Preadipocytes by Targeting KLF8 in Goats

Main regulatory factors of marbling level in beef cattle

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