Fat color and reflectance spectra to evaluate the β-carotene, lutein and α-tocopherol in the plasma of bovines finished on meadows or on a dry total mixed ration

Ripoll, G.; Casasús, I.; Joy, M.; Gahete, Francisco Molino; Alibés, Mireia Blanco

doi:10.1016/j.anifeedsci.2015.05.014

Cited by 14 publications

(5 citation statements)

References 38 publications

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“…These laboratory methods have measured the fatty acid composition of muscle and subcutaneous fat and the concentration of β‐carotene. [ 8–13 ] The disadvantage of utilising these methods is the inability to perform these tests in a processing facility, as they require extensive sample preparation. These methods, however, can provide a reference test that can be utilised to evaluate the effectiveness of new technologies, which are able to provide rapid results.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Raman spectra for the verification of Australian grass‐ and grain‐fed beef using principal component analysis and partial least square models

Logan

Hopkins

Schmidtke

et al. 2020

J Raman Spectroscopy

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The diet of cattle has been shown to alter both subcutaneous fat and muscle fatty acid composition, and these differences cause a change in the Raman spectra of the fat due to the different chemical bonds associated with these biochemical changes. Because biochemical changes only lead to subtle changes in the Raman spectra, statistical methods based upon chemometric modelling are necessary to extract indicative information on the type of feed consumed. This investigation undertook a feasibility study using a limited sample set derived from two producers in one location to ascertain the potential for Raman spectroscopy to accurately discriminate grain‐fed cattle from grass‐fed cattle and investigated the usefulness of principal component analysis (PCA) and partial least squares discriminate analysis (PLS‐DA) to best discriminate between feeding groups using the information from the Raman spectra. The first two principal components accounted for 83% of the variation in the spectra and demonstrated discrimination of samples by feed type. PLS‐DA resulted in a model that was able to accurately predict grain‐ and grass‐fed carcases with a misclassification rate of 3.5%. Changes in beef cattle diets lead to subtle changes in subcutaneous fat; using Raman spectroscopy with chemometric modelling, these changes can be identified and used to identify the production system of beef products. This study successfully uses Raman spectroscopy as an automated, nondestructive and rapid technique in the range of 600–2000 cm−1 in combination with pattern recognition of unsupervised (PCA) and supervised (PLS‐DA) techniques to classify the production system of cattle between grain and grass fed.

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Section: Introductionmentioning

confidence: 99%

Analysis of Raman spectra for the verification of Australian grass‐ and grain‐fed beef using principal component analysis and partial least square models

Logan

Hopkins

Schmidtke

et al. 2020

J Raman Spectroscopy

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“…No significant differences were found between animals fed natural milk (CM and MG), and animals fed a milk replacer (MR), which was in agreement with the results of Ripoll et al [ 5 , 46 ]. The SUM is an index related to the carotenoid content in fat [ 33 ], and because of this, it has been used as a food traceability biomarker in lambs [ 20 , 33 , 45 , 47 , 48 ] to differentiate between management systems (grazing vs. concentrate) [ 20 , 21 ]. The results from the current study agree in part with those from previous studies [ 20 , 21 ] because animals from grass-based feeding regimes had higher SUM values than animals from concentrate-based feeding regimes.…”

Section: Resultsmentioning

confidence: 99%

Retinol and α-Tocopherol Contents, Fat Color, and Lipid Oxidation as Traceability Tools of the Feeding System in Suckling Payoya Kids

Roncero-Díaz

Doblado

Córdoba

et al. 2022

Animals

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The effects of Payoya kid feeding systems on the fat-soluble vitamin (retinol/α-tocopherol) contents, fat content, fat color, and the oxidation index were evaluated to determine their potential for use as feeding system traceability tools. Four groups of Payoya kids (55 animals in total) fed milk exclusively were studied: a group fed a milk replacer (MR) and three groups fed natural milk from dams reared with different management systems (mountain grazing (MG), cultivated meadow (CM) and total mixed ration (TMR)). Kids were slaughtered around one month of age and 8 kg of live weight. Kids from the MG and CM groups presented lower retinol (5.56 and 3.72 µg/mL) and higher α-tocopherol plasma (11.43 and 8.85 µg/mL) concentrations than those from the TMR and MR groups (14.98 and 22.47 µg/mL of retinol; 2.49 and 0.52 µg/mL of α-tocopherol, respectively) (p < 0.001). With respect to fat, kids with a higher intramuscular fat percentage (CM and TMR groups) had lower retinol contents (16.52 and 15.99 µg/mL, respectively) than kids from the MG and MR groups (26.81 and 22.63 µg/mL, respectively) (p < 0.001). A dilution effect of vitamins on fat was shown: the higher the amount of fat, the lower the vitamin concentrations, the higher the lipid oxidation index (MDA), and the lower the SUM (absolute value of the integral of the translated spectra between 450 and 510 nm). A discriminant analysis that included all studied variables showed that 94.4% of the kids were classified correctly according to their feeding system and could allow traceability to the consumer.

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“…The content of α ‐tocopherol was determined by liquid extraction in duplicate as described by Lyan et al , with the modifications detailed by Ripoll et al , followed by high‐performance liquid chromatography (HPLC) (1100 Series, Agilent, Karlsruhe, Germany) using a Kinetex column (RP C18, 100 mm × 4.6 mm, 2.6 µm) and a Krudkatcher Ultra HPLC in‐line filter. α ‐Tocopherol was detected at 295 nm with a photodiode array detector.…”

Section: Methodsmentioning

confidence: 99%

Is meat quality of forage‐fed steers comparable to the meat quality of conventional beef from concentrate‐fed bulls?

et al. 2017

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Fat color and reflectance spectra to evaluate the β-carotene, lutein and α-tocopherol in the plasma of bovines finished on meadows or on a dry total mixed ration

Cited by 14 publications

References 38 publications

Analysis of Raman spectra for the verification of Australian grass‐ and grain‐fed beef using principal component analysis and partial least square models

Analysis of Raman spectra for the verification of Australian grass‐ and grain‐fed beef using principal component analysis and partial least square models

Retinol and α-Tocopherol Contents, Fat Color, and Lipid Oxidation as Traceability Tools of the Feeding System in Suckling Payoya Kids

Is meat quality of forage‐fed steers comparable to the meat quality of conventional beef from concentrate‐fed bulls?

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