Fat body is the site of vitellogenin synthesis in the soft tick,Ornithodoros moubata

Chinzei, Yasuo; Yano, Isao

doi:10.1007/bf00694580

Cited by 53 publications

(31 citation statements)

References 16 publications

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“…Authors described this Vg synthesis cascade induced by hemolymph amino acid as an amino acid signaling (Hansen et al 2004;Attardo et al 2005). Vg is also synthesized by the tick fat body after female adults obtain a blood meal, released into the hemolymph, and then transported and incorporated into eggs as vitellin (Chinzei et al 1983;Chinzei and Yano 1985;Chinzei and Taylor 1990;Taylor et al 1991;Rosell and Coons 1992;James and Oliver 1996;Taylor and Chinzei 2002;Friesen and Reuben Kaufman 2002;Gudderra et al 2002). Because amino acids in the tick are also as important as in the mosquito for Vg synthesis and oocyte development (vitellogenesis), the phenotypic changes, including reduced ability of egg production, in HlLAP RNAi ticks may be due to a reduction, not ablation (because of the existence of other aminopeptidase activity; see Fig.…”

Section: Discussionmentioning

confidence: 98%

RNA interference of cytosolic leucine aminopeptidase reduces fecundity in the hard tick, Haemaphysalis longicornis

et al. 2006

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Ticks are effective vectors of pathogens because of their blood feeding and high fecundity. This high fecundity is related to the size of the blood meal. Therefore, knowledge of how blood proteins are degraded and converted to proteins, including yolk protein, is important for the development of ways to inhibit the utilization of blood proteins by ticks. RNA interference (RNAi) is becoming a powerful post-transcriptional gene silencing technique that provides insight into gene function. We constructed a double-stranded RNA (dsRNA) based on a previously cloned Haemaphysalis longicornis leucine aminopeptidase (HlLAP) gene to reevaluate the biological role in tick blood digestion. Gene specific transcriptional, translational, and functional disruptions were achieved by the introduction of dsRNA into the ticks. Significantly delayed onset of egg-laying and reduced egg oviposition resulted from the RNAi for the HlLAP gene. These results suggest that HlLAP actually works as a blood digestive enzyme and affects tick fecundity via unknown mechanisms. The reduction of egg oviposition may be caused by a decrease in nutrients, especially free amino acids generated by HlLAP, from the blood meal. This is the first report of an impact on tick reproduction caused by gene silencing of a blood digestion-related molecule.

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Section: Discussionmentioning

confidence: 98%

RNA interference of cytosolic leucine aminopeptidase reduces fecundity in the hard tick, Haemaphysalis longicornis

et al. 2006

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“…Native and SDS-PAGE was performed followed by fluorography as described elsewhere (Chinzei and Yano, 1985). For the quantitative analysis of Vg synthetic activities HL was run by RIE with anti-(Vn-1) serum.…”

Section: Analysis Of In Vivo Vg Synthesismentioning

confidence: 99%

Vitellogenins and vitellins in the bean bug,Riptortus clavatus (hemiptera: alydidae): Purification, immunological identification, and induction by juvenile hormone

Shinoda

Miura

Taylor

et al. 1996

Arch. Insect Biochem. Physiol.

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Vitellogenins (Vgs) and vitellins (Vns) were purified from reproductive female adult hemolymph (HL) and newly laid eggs of the bean bug, Riptortus clavatus. They were separated into two (Vg‐1 and 2) and three (Vn‐1′, Vn‐1, and Vn‐2) sub‐components, respectively, by ion exchange chromatography. Fused rocket immunoelectrophoresis using anti‐(Vn‐1) serum showed that they are distinguished clearly into two immunologically distinct groups, i.e., Vg‐1/Vn‐1/Vn‐1′ and Vg‐2/Vn‐2. SDS‐PAGE analysis showed Vn‐1 (Vn‐1′) and Vn‐2 have the same and/or smaller subunit component polypeptides as Vg‐1 and Vg‐2, respectively. Vn‐1′ has some lower molecular weight peptides than Vn‐1. Quantitative rocket immunoelectrophoretic analysis showed that Vg‐1 and Vn‐1 (plus V‐1′) were detected first on day 3 in the HL and day 4 in the ovary, respectively, after adult emergence, and increased by day 10 in non‐diapause female adults. Vg‐1 synthesis is induced in diapause female adults in 1 day after the treatment with juvenile hormone (JH) and JH analog (JHA) or in a few days after transfer from short day to long day conditions. © 1996 Wiley‐Liss, Inc.

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“…In ticks, the synthesis of vitellogenin has been studied in the ixodids Rhipicephalus sanguineus, Dermacentor variabilis and Hyalomma dromedarii (Araman, 1979;Coons et al, 1986;Rosell and Coons, 1989;Schriefer, 1991) and in the argasids Ornithodoros moubata and Ornithodoros parkeri (Chinzei and Yano, 1985;Taylor et al, 1991). Vitellogenin in ixodids and argasids is released into the haemolymph and is selectively taken up by the ovary and deposited as the major yolk protein, vitellin.…”

Section: Introductionmentioning

confidence: 99%

“…Vitellogenin in ixodids and argasids is released into the haemolymph and is selectively taken up by the ovary and deposited as the major yolk protein, vitellin. The fat body is the principal site of vitellogenin production in R. sanguineus, O. moubata, and H. dromedarii (Araman, 1979;Chinzei and Yano, 1985;Schriefer, 1991). In D. variabilis, the fat body and midgut may both be sources of vitellogenin production (Rosell and Coons, 1992).…”

Section: Introductionmentioning

confidence: 99%

Vitellogenin concentrations in the haemolymph and ovaries of Ixodes scapularis ticks during vitellogenesis

James

Oliver

1996

Exp Appl Acarol

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The vitellogenin and vitellin concentrations in the haemolymph and ovaries of Ixodes scapularis females were determined using a double sandwich enzyme-linked immunosorbent assay. The level of vitellogenin in the haemolymph began to increase just prior to tick detachment from the host and continued to increase until 2 days after detachment. There was a slight decrease in the vitellogenin level 4 days after detachment, but a second peak was observed approximately 5 days after oviposition. Subsequent to oviposition, the vitellogenin levels in the haemolymph quickly decreased. The concentration of vitellogenin in the haemolymph ranged from 1.55 to 11.48 micrograms microliters-1 during the period after dropping from the host through oviposition. The concentration of vitellin in the ovaries began to increase as the female began rapid engorgement (0.03 mg per female) and declined after oviposition (0.1 mg per female).

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Fat body is the site of vitellogenin synthesis in the soft tick,Ornithodoros moubata

Cited by 53 publications

References 16 publications

RNA interference of cytosolic leucine aminopeptidase reduces fecundity in the hard tick, Haemaphysalis longicornis

RNA interference of cytosolic leucine aminopeptidase reduces fecundity in the hard tick, Haemaphysalis longicornis

Vitellogenins and vitellins in the bean bug,Riptortus clavatus (hemiptera: alydidae): Purification, immunological identification, and induction by juvenile hormone

Vitellogenin concentrations in the haemolymph and ovaries of Ixodes scapularis ticks during vitellogenesis

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