Fasting-induced depletion of glutathione in the aging mouse

Vogt, Barbara L.; Richie, John P.

doi:10.1016/0006-2952(93)90412-p

Cited by 58 publications

(31 citation statements)

References 34 publications

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“…This constraint was applied in order to avoid differential amounts of food consumed by animals of different ages in different states of intoxication, a possibility not commonly accounted for in previous studies. Hepatic GSH levels are greatly affected by diet and fasting alone severely depletes hepatic GSH stores in as little as 6 hrs [34]. Fasting-induced GSH depletion in the liver is thought to result from a reduction in its de novo biosynthesis due to decreased levels of circulating Cys, its rate-limiting precursor.…”

Section: Discussionmentioning

confidence: 99%

“…Male C57Bl/6NNIA mice of ages 6 months (young), 12 months (mature adults), and 24 months (old) were obtained from colonies administered by the National Institute on Aging, as described previously [34]. Prior to experimentation, which began at 8 a.m., mice had free access to food (NIH-07) and water.…”

Section: Experimental Animalsmentioning

confidence: 99%

“…Previous results from this laboratory and others have demonstrated that a GSH deficiency is a common phenomenon of senescent organisms, including humans [27][28][29][30][31]. While the mechanisms responsible for this aging impairment are unknown, several studies indicate that a decrease in turnover of GSH and a loss of biosynthetic capacity may be involved [32][33][34]. Based on these results and on the numerous functions of GSH in cellular homeostasis and defense [35], a loss of GSH may represent a critical factor in the aging process [36].…”

Section: Introductionmentioning

confidence: 96%

“…However, in this study, only one dose of EtOH was examined and the confounding factor of fasting was not taken into account in the experimental design. Indeed, fasting is known to impact hepatic GSH levels and hepatotoxicity [38,39] and we have observed that fasting-induced GSH depletion was greater in old than in younger animals [34]. In another study, effects of age were examined in younger age groups, so that results reflected growth and maturational changes rather than the aging process per se [40].…”

Section: Introductionmentioning

confidence: 97%

“…To test this, GSH concentrations in various tissues were determined in young, mature and old mice throughout a 24-hr time course after acute EtOH treatment, and after increasing doses of EtOH. Special attention was placed on comparison of EtOH-treated mice with time-matched controls, to account for the decreases in GSH resulting from food deprivation [34]. Also examined were the effects of EtOH on the tissue concentrations of GSH related metabolites, cyst(e)ine and glutathione disulfide (GSSG).…”

Section: Introductionmentioning

confidence: 99%

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Glutathione depletion and recovery after acute ethanol administration in the aging mouse

Vogt

Richie

2007

Biochemical Pharmacology

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Glutathione (GSH) plays an important role in the detoxification of ethanol (EtOH) and acute EtOH administration leads to GSH depletion in the liver and other tissues. Aging is also associated with a progressive decline in GSH levels and impairment in GSH biosynthesis in many tissues. Thus, the present study was designed to examine the effects of aging on EtOH-induced depletion and recovery of GSH in different tissues of the C57Bl/6NNIA mouse. EtOH (2-5 g/kg) or saline was administered i.p. to mice of ages 6 mo (young), 12 mo (mature), and 24 mo (old); and GSH and cyst(e)ine concentrations were measured 0-24 hours thereafter. EtOH administration (5g/kg) depleted hepatic GSH levels >50% by 6 hr in all animals. By 24 hr, levels remained low in both young and old mice, but recovered to baseline levels in mature mice. At 6 hr, the decrease in hepatic GSH was dosedependent up to 3 g/kg EtOH, but not at higher doses. The extent of depletion at the 3 g/kg dose was dependent upon age, with old mice demonstrating significantly lower GSH levels than mature mice (P<0.001). Altogether these results indicate that aging was associated with a greater degree of EtOH and fasting-induced GSH depletion and subsequent impaired recovery in liver. An impaired ability to recover was also observed in young animals. Further studies are required to determine if an inability to recover from GSH depletion by EtOH is associated with enhanced toxicity.

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Section: Discussionmentioning

confidence: 99%

Section: Experimental Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Glutathione depletion and recovery after acute ethanol administration in the aging mouse

Vogt

Richie

2007

Biochemical Pharmacology

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Effect of maternal or post‐weaning methyl donor supplementation on growth performance, carcass traits, and meat quality of pig offspring

et al. 2018

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BACKGROUND: Limited studies have examined links between maternal methyl donor (MET) supplementation and the growth-development characteristics of offspring, and possible underlying mechanisms for such links. This study investigated the effect of maternal or post-weaning MET-supplementation on growth performance, carcass characteristics, and meat quality of the finishing (d 180) offspring. Twenty-four sows were placed on a control (C) or MET-supplemented diet during pregnancy and lactation. Forty-eight female offspring were fed the control or MET-supplemented diet from weaning to 6 months of age, resulting in four study groups (six litters per group): C/C, C/MET, MET/C, and MET/MET. RESULTS: Maternal MET-supplementation increased average daily gain (ADG), body weight (BW), lean percentage and longissimus dorsi (LD) of the offspring at day 180 (P < 0.05), and upregulated the myosin heavy chain IIx, myogenic differentiation and muscle regulatory factor 4 mRNA levels in the LD muscle (P < 0.05). Meanwhile, offspring from maternal MET-supplementation exhibited a higher pH 24h post mortem and superoxide dismutase activity, a lower L * 45min , glycolytic potential, malonaldehyde content in the LD muscle, and plasma homocysteine concentration (P < 0.05). CONCLUSION: Maternal MET-supplementation has a remarkable effect on growth performance, carcass traits, and meat quality of the offspring, which is associated with increased expression levels of myogenic genes and anti-oxidant capacity.

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