Fasting and refeeding impacts on piglet jejunal transcriptome during weaning period

Mazurais, David; Rome, Véronique; Cahu, Armelle; Gadie,; Huërou‐Luron, Isabelle Le

doi:10.1016/j.livsci.2007.01.006

Cited by 3 publications

(5 citation statements)

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“…Thus, it would be necessary to link mRNA measurements with specific individual cell types (for example, by means of micro-dissection or immune histological techniques) (159) . In consequence, although a direct correlation between transcriptomic regulation and functional impact is not always easy to establish, studies exist suggesting fasting/ refeeding-induced gene regulations (for example, Mazurais et al (35) ) to be consistent with alterations in the small intestine in piglets at weaning (for example, Marion et al (210) ; Boudry et al (24) ). Thus, although given the limitation of gene expression studies, for example, need for comparisons between changes in gene expression with other, for example, morphological or zootechnical parameters, use of gene expression studies will help to gain deeper insight into the mechanism governing the physiological functions and their regulation in livestock species (207) .…”

Section: Limitations and Strengths Of Gene Expression To Determine Immentioning

confidence: 99%

“…Such weaning-associated changes in the intestinal microbiota can, in turn, also influence immune development ( 34 ) . However, the molecular mechanisms underlying weaning anorexia and refeeding are not well established in piglets ( 35 ) . Recent studies which used microarray techniques have shown that weaning in piglets coincides with marked alterations in the expression of various intestinal genes, related to nutrient metabolism and/or cell proliferation and/or immune function ( 7 , 36 ) .…”

Section: The Weaning Transition: a Brief Summarymentioning

confidence: 99%

“…Recent studies which used microarray techniques have shown that weaning in piglets coincides with marked alterations in the expression of various intestinal genes, related to nutrient metabolism and/or cell proliferation and/or immune function ( 7 , 36 ) . Such information would be relevant for improving the understanding of post-weaning gut disorders ( 35 ) .…”

Section: The Weaning Transition: a Brief Summarymentioning

confidence: 99%

“…Alterations of intestinal integrity, however, were also observed in underfed piglets and piglets receiving TPN, when compared with their counterparts being fed ad libitum or receiving enteral nutrition, independently of the weaning process ( 26 , 56 ) . Mazurais et al ( 35 ) investigated the impact of fasting and refeeding on the piglet's jejunal transcriptome during the weaning period. In their study, 21-d-old sow-reared piglets were fasted for 2 d before slaughtering at the age of 23 d. Porcine arrays associated with real-time PCR were used to assess global gene expression changes in the jejunum of piglets at weaning.…”

Section: Changes In Intestinal Gene Expression Due To Reduced Feed Inmentioning

confidence: 99%

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Intestinal gene expression in pigs: effects of reduced feed intake during weaning and potential impact of dietary components

et al. 2011

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The weaning transition is characterised by morphological, histological and microbial changes, often leading to weaning-associated disorders. These intestinal changes can partly be ascribed to the lack of luminal nutrition arising from the reduced feed intake common in pigs after weaning. It is increasingly becoming clear that changes in the supply with enteral nutrients may have major impacts on intestinal gene expression. Furthermore, the major dietary constituents, i.e. carbohydrates, fatty acids and amino acids, participate in the regulation of intestinal gene expression. However, nutrients may also escape digestion by mammalian enzymes in the upper gastrointestinal tract. These nutrients can be used by the microflora, resulting in the production of bacterial metabolites, for example, SCFA, which may affect intestinal gene expression indirectly. The present review provides an insight on possible effects of reduced feed intake on intestinal gene expression, as it may occur post-weaning. Detailed knowledge on effects of reduced feed intake on intestinal gene expression may help to understand weaning-associated intestinal dysfunctions and diseases. Examples are given of intestinal genes which may be altered in their expression due to supply with specific nutrients. In that way, gene expression could be modulated by dietary means, thereby acting as a potential therapeutic tool. This could be achieved, for example, by influencing genes coding for digestive or absorptive proteins, thus optimising digestive function and metabolism, but also with regard to immune response, or by influencing proliferative processes, thereby enhancing mucosal repair. This would be of special interest when designing a diet to overcome weaning-associated problems.

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Section: Limitations and Strengths Of Gene Expression To Determine Immentioning

confidence: 99%

Section: The Weaning Transition: a Brief Summarymentioning

confidence: 99%

Section: The Weaning Transition: a Brief Summarymentioning

confidence: 99%

Section: Changes In Intestinal Gene Expression Due To Reduced Feed Inmentioning

confidence: 99%

See 2 more Smart Citations

Intestinal gene expression in pigs: effects of reduced feed intake during weaning and potential impact of dietary components

et al. 2011

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show abstract

“…Zhang et al reported that pigs submitted to different feeding frequencies, 1, 3 or 5 meals per day, have a different ileal and colonic mucosal transcriptome 20 , although they only investigated transcriptomes from overnight fasted pigs. Mazurais et al studied the impact of a 2-day fasting in the jejunum expression levels of targeted genes in piglets, and noted 146 affected over the 954 studied genes 21 . In mice, Yoshioka et al have investigated the effect of a high-fat and low-fat diet on the duodenal mucosa compared to fasted mice 22 .…”

Section: Introductionmentioning

confidence: 99%

Divergent selection for feed efficiency in pigs altered the duodenum transcriptome and DNA methylation profiles, resulting in greater responses to feed intake in the most efficient line

Devailly

Fève

Saci

et al. 2022

Preprint

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Feed efficiency is a trait of interest in pigs as it contributes to lowering the ecological and economical costs of pig production. A divergent genetic selection experiment from a Large White pig population was performed for 10 generations, leading to pig lines with relative low- (LRFI, more efficient) and high- (HRFI, less efficient) residual feed intake (RFI). The meals of pigs from the LRFI line are shorter and less frequent as compared to the HRFI line. We hypothesised that these differences in feeding behaviour could be related to differential sensing and absorption of nutrients in the intestine. Here we investigated the duodenum transcriptomic response to short term feed intake in LRFI and HRFI lines (n=24). We identified 1106 differentially expressed genes between the two lines, notably affecting pathways of the transmembrane transport activity and related to mitosis or chromosome separation. The LRFI line showed a greater transcriptomic response to feed intake, with 2222 differentially expressed genes before and after a meal, as compared to 61 differentially expressed genes in the HRFI line. Feed intake affected genes from both anabolic and catabolic pathways in the pig duodenum, such as autophagy and rRNA production. We noted that several nutrient transporter genes were differentially expressed between lines and/or by short term feed intake. Altogether, our findings highlighted that the genetic selection for feed efficiency in pigs changed the transcriptome profiles of the duodenum, and notably its response to feed intake.

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Divergent selection for feed efficiency in pigs altered the duodenum transcriptomic response to feed intake and its DNA methylation profiles

Devailly,

Fève,

Saci

et al. 2024

Physiological Genomics

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Feed efficiency is a trait of interest in pigs as it contributes to lowering the ecological and economical costs of pig production. A divergent genetic selection experiment from a Large White pig population was performed for 10 generations, leading to pig lines with relative low- (LRFI) and high- (HRFI) residual feed intake (RFI). Feeding behavior and metabolic differences have been previously reported between the two lines. We hypothesized that part of these differences could be related to differential sensing and absorption of nutrients in the proximal intestine. We investigated the duodenum transcriptome and DNA methylation profiles comparing overnight fasting with ad libitum feeding in LRFI and HRFI pigs (n=24). We identified 1,106 differentially expressed genes between the two lines, notably affecting pathways of the transmembrane transport activity and related to mitosis or chromosome separation. The LRFI line showed a greater transcriptomic response to feed intake than the HRFI line. Feed intake affected genes from both anabolic and catabolic pathways in the pig duodenum, such as rRNA production and autophagy. Several nutrient transporter and tight junction genes were differentially expressed between lines and/or by short term feed intake. We also identified 409 differentially methylated regions in the duodenum mucosa between the two lines, while this epigenetic mark was less affected by feeding. Our findings highlighted that the genetic selection for feed efficiency in pigs changed the transcriptome profiles of the duodenum, and notably its response to feed intake, suggesting key roles for this proximal gut segment in mechanisms underlying feed efficiency.

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Fasting and refeeding impacts on piglet jejunal transcriptome during weaning period

Cited by 3 publications

References 10 publications

Intestinal gene expression in pigs: effects of reduced feed intake during weaning and potential impact of dietary components

Intestinal gene expression in pigs: effects of reduced feed intake during weaning and potential impact of dietary components

Divergent selection for feed efficiency in pigs altered the duodenum transcriptome and DNA methylation profiles, resulting in greater responses to feed intake in the most efficient line

Divergent selection for feed efficiency in pigs altered the duodenum transcriptomic response to feed intake and its DNA methylation profiles

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