Fasting and Postprandial Plasma Concentrations of Acylation‐Stimulation Protein (ASP) in Lean and Obese Pima Indians Compared to Caucasians

Weyer, Christian; Pratley, Richard E.

doi:10.1002/j.1550-8528.1999.tb00432.x

Cited by 40 publications

(35 citation statements)

References 37 publications

(28 reference statements)

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“…Such a function is supported by data indicating that ASP administration increases triglyceride clearance from plasma, 8 and that ASPdeficient (C3À/À) mice exhibit delayed postprandial lipid clearance, reduced adipose tissue depots and are resistant to obesity induced by high-fat diet. 9,10 Involvement by ASP in the regulation of energy storage is also supported by reports that plasma levels of ASP are increased in obesity 11 and reduced in fasting 12 and in postobese women. 13 Adipose tissue release of ASP increases postprandially, with a peak release observed at 4-5 h after meal ingestion, although circulating levels remain constant.…”

Section: Introductionmentioning

confidence: 71%

Acylation stimulating protein stimulates insulin secretion

et al. 2003

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Acylation stimulating protein (ASP) is a hormone produced by adipocytes and is of importance for the storage of energy as fat. We examined whether ASP might also have effects on islet function. In clonal INS-1 cells, ASP dose-dependently augmented glucose-stimulated insulin secretion. The lowest effective dose of ASP at 10 mmol/l glucose was 5 mmol/l. The effect was glucosedependent because ASP did not increase insulin secretion at 1 mmol/l glucose but had clear effect at 10 and 20 mmol/l glucose. Similarly, ASP augmented glyceraldehyde-induced insulin secretion but the hormone did not enhance insulin secretion in response to depolarization by 20 mmol/l of KCl. ASP-induced insulin secretion was completely abolished by competitive inhibition of glucose phosphorylation by glucokinase with 5-thio-glucose and was partially inhibited by the calcium channel blocker, nifedipine, and by the protein kinase C inhibitor, GF109203. Furthermore, thapsigargin, an inhibitor of Ca 2+ -ATPase in the endoplasmic reticulum, did not affect ASP-induced insulin secretion. ASP (45 mmol/l) also augmented glucose-stimulated insulin secretion from islets isolated from C57BL/6J mice, and intravenous administration of ASP (50 nmol/kg) augmented the acute (1 and 5 min) insulin response to intravenous glucose (1 g/kg) in C57BL/6J mice. This was accompanied by an increased rate of glucose disposal. Minimal model analyses of data derived from the intravenous glucose tolerance test revealed that whereas ASP augmented insulin secretion, the hormone did not affect insulin sensitivity (S I ) or glucose effectiveness (S G ). We conclude that ASP augments glucose-stimulated insulin secretion through a direct action on the islet beta cells. The effect is dependent on glucose phosphorylation, calcium uptake and protein kinase C. Stimulation of insulin secretion by ASP in vivo results in augmented glucose disposal.

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Section: Introductionmentioning

confidence: 71%

Acylation stimulating protein stimulates insulin secretion

et al. 2003

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“…(19)], to date, only five studies with limited numbers of subjects have examined postprandial ASP. In those studies, postprandial ASP either remained constant (20,23,24) or decreased over the fat load (21,22). In previous studies, the drop in ASP generally occurred in those subjects who had the highest fasting plasma ASP (such as obese and diabetic subjects) (20,21), but only one report showed fasting ASP correlating with postprandial TG and NEFA (20).…”

Section: Discussionmentioning

confidence: 98%

“…Previous studies on postprandial ASP have demonstrated little significant change in circulating ASP (20)(21)(22)(23)(24). On the other hand, arterio-venous studies in human subcutaneous adipose tissue have demonstrated increased release of ASP from human subcutaneous adipose tissue, and the degree to which this occurs correlates with the extent of fatty acid trapping by adipose tissue (19,25,26).…”

mentioning

confidence: 99%

Fasting acylation-stimulating protein is predictive of postprandial triglyceride clearance

Cianflone

Zakarian

Couillard

et al. 2004

Journal of Lipid Research

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Postprandial plasma triglyceride (ppTG) and NEFA clearance were stratified by plasma acylation-stimulating protein (ASP) and gender to determine the contribution of fasting ASP in a normal population (70 men; 71 women). In the highest ASP tertile only, ASP decreased over 8 h (90 ؎ 9.7 nM to 70 ؎ 5.9 nM, P Ͻ 0.05 males; 61.9 ؎ 4.0 nM to 45.6 ؎ 6.2 nM, P Ͻ 0.01 females). Fasting ASP correlated positively with ppTG response. ppTG ( P Ͻ 0.0001, 2-way ANOVA, both genders) and NEFA levels progressively increased from lowest to highest ASP tertile, with the greatest differences in males. By stepwise multiple regression, the best prediction of ppTG was: (fasting ASP ؉ apolipoprotein B ؉ insulin ؉ TG; r ‫؍‬ 0.806) for men and (fasting ASP ؉ total cholesterol; r ‫؍‬ 0.574) for women. Leptin, body mass index, and other fasting variables did not improve the prediction. Thus, in men and women, ASP significantly predicted ppTG and NEFA clearance and, based on lower ASP, women may be more ASP sensitive than men. Plasma ASP may be useful as a fasting variable that will provide additional information regarding ppTG and NEFA clearance.

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“…Interestingly, however, several studies have shown that ASP levels are high in obese subjects, possibly as a direct consequence of increased fat mass. 30,29,11,12 It was recently hypothesized that these high ASP levels represent an ASP-resistant state, whereas low ASP levels indicate increased sensitivity of adipose tissue towards ASP. 14 This hypothesis is supported by in vitro data.…”

Section: Discussionmentioning

confidence: 99%

“…10 Altogether, these data suggest that ASP might be an important factor in regulating fatty acid metabolism in white adipose tissue and thereby also controlling plasma fatty acid levels. In this context, obese and type 2 diabetic humans have increased levels of plasma ASP, 11,12 and these subjects are characterized by disordered fatty acid metabolism and insulin resistance. 13 Therefore, Cianflone et al 14 recently suggested that increased plasma ASP levels might be a marker of cellular ASP resistance.…”

Section: Introductionmentioning

confidence: 99%

Acylation-stimulating protein: effect of acute exercise and endurance training

et al. 2005

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INTRODUCTION: Acylation-stimulating protein (ASP) is an adipocyte-derived protein that contributes to fatty acid clearance. Regular exercise training improves fatty acid handling. OBJECTIVE: To examine the effect of acute exercise and short-term endurance training on ASP levels. SUBJECTS: Eight untrained men (age: 23.573.4 y; maximal power output (W max ): 3.770.6 W/kg body weight). DESIGN: Subjects were trained for 2 weeks. Before and after training, blood was sampled during a 3-h exercise test, and insulin sensitivity was assessed by an insulin tolerance test. RESULTS: Before training, ASP levels decreased during exercise (from 17.972.9 to 15.573.7 nmol/l at t ¼ 0 vs 180, Po0.05). Endurance training decreased fasting ASP levels significantly (17.972.9 vs 13.472.3 nmol/l pre-and post-training, Po0.001). Interestingly, after 2 weeks of endurance training, ASP levels tended to increase during exercise (from 13.472.3 to 17.274.5 nmol/l at t ¼ 0 vs 180, P ¼ 0.09). Baseline ASP levels correlated negatively with insulin sensitivity both before (r ¼ À0.86, Po0.01) and after training (r ¼ À0.82, Po0.05). CONCLUSION: Short-term endurance training reduces baseline ASP levels. These data fit with the hypothesis that reduced ASP levels indicate improved ASP sensitivity.

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Fasting and Postprandial Plasma Concentrations of Acylation‐Stimulation Protein (ASP) in Lean and Obese Pima Indians Compared to Caucasians

Cited by 40 publications

References 37 publications

Acylation stimulating protein stimulates insulin secretion

Acylation stimulating protein stimulates insulin secretion

Fasting acylation-stimulating protein is predictive of postprandial triglyceride clearance

Acylation-stimulating protein: effect of acute exercise and endurance training

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