“Fasting: An Effective Preconditioning Method to Increase Fat Graft Survival”

Gyu, Han; Kim, Dong Gyu; Chang, Jiyeon; Song, Yuri; Jeong, Seongfeel; Nam, Seung Min; Wee, Syeo Young; Cho, Kae Won; Choi, Chang Yong

doi:10.1007/s00266-021-02630-8

Cited by 7 publications

(5 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additional histological assessment of the grafted tissue showed no significant differences in adipocyte morphology for grafts with and without milled PLGA fibers (Figure S4A–C). However, our western blot results indicate that the lipolytic activity of human adipose tissue was significantly increased in the absence of milled PLGA fibers (Figure S4D, E), which could also potentially be a contributing factor to improved volume retention in the presence of milled fibers 36,37 …”

Section: Resultsmentioning

confidence: 90%

“…Lipolysis, on the other hand, is a contributing factor in the reduction of adipocyte volume and leads to the overall decrease of adipose tissue size. 36,37 Because lipolysis could therefore be a factor in adipocyte morphology and the retention of graft volume, we investigated the effects of milled electrospun PLGA fibers on lipolytic activity in adipose tissue via western blot analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Comparisons of maximum Feret's diameter and cell area made between adipose grafts with and without milled PLGA fibers revealed no significant differences in either metric at 21 days, suggesting that improved volume retention is likely not driven by changes in adipocyte size. Lipolysis, on the other hand, is a contributing factor in the reduction of adipocyte volume and leads to the overall decrease of adipose tissue size 36,37 . Because lipolysis could therefore be a factor in adipocyte morphology and the retention of graft volume, we investigated the effects of milled electrospun PLGA fibers on lipolytic activity in adipose tissue via western blot analysis.…”

Section: Discussionmentioning

confidence: 99%

“…However, our western blot results indicate that the lipolytic activity of human adipose tissue was significantly increased in the absence of milled PLGA fibers (Figure S4D, E), which could also potentially be a contributing factor to improved volume retention in the presence of milled fibers. 36,37…”

Section: Co-grafting Of Human Adipose Tissue With Milled Plga Fibers ...mentioning

confidence: 99%

See 3 more Smart Citations

Injectable pulverized electrospun poly(lactic‐co‐glycolic acid) fibers improve human adipose tissue engraftment and volume retention

Das,

Lawrence,

Diaz‐Starokozheva

et al. 2023

J Biomedical Materials Res

View full text Add to dashboard Cite

Autologous adipose tissue is commonly used for tissue engraftment for the purposes of soft tissue reconstruction due to its relative abundance in the human body and ease of acquisition using liposuction methods. This has led to the adoption of autologous adipose engraftment procedures that allow for the injection of adipose tissues to be used as a “filler” for correcting cosmetic defects and deformities in soft tissues. However, the clinical use of such methods has several limitations, including high resorption rates and poor cell survivability, which lead to low graft volume retention and inconsistent outcomes. Here, we describe a novel application of milled electrospun poly(lactic‐co‐glycolic acid) (PLGA) fibers, which can be co‐injected with adipose tissue to improve engraftment outcomes. These PLGA fibers had no significant negative impact on the viability of adipocytes in vitro and did not elicit long‐term proinflammatory responses in vivo. Furthermore, co‐delivery of human adipose tissue with pulverized electrospun PLGA fibers led to significant improvements in reperfusion, vascularity, and retention of graft volume compared to injections of adipose tissue alone. Taken together, the use of milled electrospun fibers to enhance autologous adipose engraftment techniques represents a novel approach for improving upon the shortcomings of such methods.

show abstract

Section: Resultsmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Co-grafting Of Human Adipose Tissue With Milled Plga Fibers ...mentioning

confidence: 99%

See 2 more Smart Citations

Injectable pulverized electrospun poly(lactic‐co‐glycolic acid) fibers improve human adipose tissue engraftment and volume retention

Das,

Lawrence,

Diaz‐Starokozheva

et al. 2023

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Besides MSC cell behavior itself, dynamic regulation of ASCs with niche is crucial factor to determine the fat graft survival rate. Previously, our group reported that VEGF-induced angiogenesis and lipolysis play an important role in regulating fat graft survival [ 53 , 54 ]. Recent studies have shown that biophysical signals can regulate the immunomodulatory functions of ASCs through cytokine secretion [ 55 ].…”

Section: Resultsmentioning

confidence: 99%

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

Chun

Chang

Soedono

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Cell-assisted lipotransfer (CAL), defined as co-transplantation of aspirated fat with enrichment of adipose-derived stem cells (ASCs), is a novel technique for cosmetic and reconstructive surgery to overcome the low survival rate of traditional fat grafting. However, clinically approved techniques for increasing the potency of ASCs in CAL have not been developed yet. As a more clinically applicable method, we used mechanical stress to reinforce the potency of ASCs. Mechanical stress was applied to the inguinal fat pad by needling . Morphological and cellular changes in adipose tissues were examined by flow cytometric analysis 1, 3, 5, and 7 days after the procedure. The proliferation and adipogenesis potencies of ASCs were evaluated. CAL with ASCs treated with mechanical stress or sham control were performed, and engraftment was determined at 4 weeks post-operation. Flow cytometry analysis revealed that mechanical stress significantly increased the number as well as the frequency of ASC proliferation in fat. Proliferation assays and adipocyte-specific marker gene analysis revealed that mechanical stress promoted proliferation potential but did not affect the differentiation capacity of ASCs. Moreover, CAL with cells derived from mechanical stress-treated fat increased the engraftment. Our results indicate that mechanical stress may be a simple method for improving the efficacy of CAL by enhancing the proliferation potency of ASCs.

show abstract

Invited Discussion on “Fasting: An Effective Preconditioning Method to Increase Fat Graft Survival”

Farajzadeh

2022

Aesth Plast Surg

View full text Add to dashboard Cite

“Fasting: An Effective Preconditioning Method to Increase Fat Graft Survival”

Cited by 7 publications

References 45 publications

Injectable pulverized electrospun poly(lactic‐co‐glycolic acid) fibers improve human adipose tissue engraftment and volume retention

Injectable pulverized electrospun poly(lactic‐co‐glycolic acid) fibers improve human adipose tissue engraftment and volume retention

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

Invited Discussion on “Fasting: An Effective Preconditioning Method to Increase Fat Graft Survival”

Contact Info

Product

Resources

About