Fasting alters protein expression of AMP-activated protein kinase in the hypothalamus of broiler chicks (Gallus gallus domesticus)

Song, Zhigang; Liu, Lei; Yue, Yunshuang; Jiao, Hongchao; Lin, Hai; Sheikhahmadi, Ardashir; Everaert, Nadia; Decuypere, Eddy; Buyse, Johan

doi:10.1016/j.ygcen.2012.06.026

Cited by 58 publications

(48 citation statements)

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“…In vitro, Shimizu et al (16) demonstrated that GCs up-regulate hypothalamic NPY and AgRP gene expression in rats through AMPK signaling, at least in part. In poultry, hypothalamic AMPK signaling can be activated under fasting conditions (17) and can be affected by various peripheral signals related to energy balance, including insulin, leptin, and ghrelin (18,19). However, whether AMPK is involved in GC-induced hyperphagia in chickens remains unknown.…”

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Glucocorticoids Increase NPY Gene Expression via Hypothalamic AMPK Signaling in Broiler Chicks

et al. 2014

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Glucocorticoids (GCs) induce profound hyperphagia in birds. However, the neuronal regulatory network underlying GC-provoked hyperphagia is unclear. To determine whether any cross talk occurs among hypothalamic GC receptors (GRs), AMP-activated protein kinase (AMPK), and GCs in the regulation of appetite, we performed an intracerebroventricular injection of mifepristone (a GR inhibitor) and compound C (an AMPK inhibitor) on GC-treated male chicks. The results indicate that central GC administration increased the expression of GR and neuropeptide Y mRNA, as well as phosphorylated AMPKα(Thr172) and acetyl-coenzyme A carboxylase(Ser79). Blocking AMPK significantly attenuated GC-induced hyperphagia. Blocking GR significantly attenuated part of the AMPK signaling pathway and GC-induced hyperphagia. Thus, the results suggest that GCs cause hyperphagia via the AMPK-neuropeptide Y signaling pathway.

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Glucocorticoids Increase NPY Gene Expression via Hypothalamic AMPK Signaling in Broiler Chicks

et al. 2014

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“…Hypothalamic POMC mRNA levels are increased by peripheral insulin and decreased by fasting in mammals (Benoit et al, 2002). Although central insulin administration could up-regulate POMC mRNA level in chicks (Honda et al, 2007), fasting for 48 h (Song et al, 2012) or peripheral leptin (Dridi et al, 2005), insulin administration has no significant effect on hypothalamic POMC mRNA levels (Song et al, 2012). The results suggest that hypothalamic POMC of chick is insensitive to peripheral metabolic signals.…”

Section: Discussionmentioning

confidence: 99%

Peripheral Insulin Doesn’t Alter Appetite of Broiler Chicks

Liu¹,

Xu²,

Wang

et al. 2016

Asian Australas. J. Anim. Sci

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An experiment was conducted to investigate the effect of peripheral insulin treatment on appetite in chicks. Six-d-age chicks with ad libitum feeding or fasting for 3 h before injection received a subcutaneous injection of 0, 1, 3, 5, 10, or 20 IU of insulin or vehicle (saline). The results showed peripheral insulin treatment (1 to 20 IU) did not alter significantly the feed intake in chicks under either ad libitum feeding or fasting conditions within 4 h (p>0.05). Compared with the control, plasma glucose concentration was significantly decreased after insulin treatment of 3, 5, 10, and 20 IU for 4 h in chicks with ad libitum feeding (p<0.05). In fasted chicks, 10 and 20 IU insulin treatments significantly decreased the plasma glucose level for 4 h (p<0.05). Peripheral insulin treatment of 10 IU for 2 or 4 h did not significantly affect the hypothalamic genes expression of neuropeptide Y, proopiomelanocortin, corticotropin-releasing factor and insulin receptors (p>0.05). All results suggest peripheral administration of insulin has no effect on appetite in chicks.

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“…Changes in mRNA appear to reflect parallel changes in peptide because food deprivation increased the number of AGRP-immunoreactive cells in the arcuate nucleus of the ring dove (Streptopelia risoria) which were also elevated during the parental phase of the breeding cycle when the birds are in negative energy balance . For POMC, Higgins et al (2010) 2003; Song et al, 2012). Thus the changes in AGRP and POMC expression during fasting are in the direction predicted from the behavioural effects of their encoded peptides as part of a coordinated counter-regulatory response of hypothalamic appetite control peptides to restore lost energy stores (Phillips-Singh et al, 2003).…”

Section: Effects Of Manipulating Energy Statusmentioning

confidence: 99%

“…Most investigations have focused on short-term food deprivation, which increases AGRP mRNA (Phillips-Singh et al, 2003;Higgins et al, 2010;Song et al, 2012). Changes in mRNA appear to reflect parallel changes in peptide because food deprivation increased the number of AGRP-immunoreactive cells in the arcuate nucleus of the ring dove (Streptopelia risoria) which were also elevated during the parental phase of the breeding cycle when the birds are in negative energy balance .…”

Section: Effects Of Manipulating Energy Statusmentioning

confidence: 99%

“…There is also suggestive evidence for a role of hypothalamic energy sensing pathways in regulating central melanocortin signalling. Proszkowiec-Weglarz et al (2006) observed strong immunostaining for the energy sensor AMP-activated protein kinase (AMPK) in the arcuate nucleus and Song et al (2012) reported changes in AMPK activity indicated by phosphorylation during food deprivation and refeeding that paralleled changes in AGRP gene expression.…”

Section: Effects Of Manipulating Energy Statusmentioning

confidence: 99%

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Regulation of the avian central melanocortin system and the role of leptin

Boswell

Dunn

2015

General and Comparative Endocrinology

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The avian central melanocortin system is well conserved between birds and mammals in terms of the component genes, the localisation of their expression in the hypothalamic arcuate nucleus, the effects on feeding behaviour of their encoded peptides and the sensitivity of agouti-related protein (AGRP) and pro-opiomelanocortin (POMC) gene expression to changes in energy status. Our recent research has demonstrated that AGRP gene expression precisely differentiates between broiler breeder hens with different histories of chronic food restriction and refeeding. We have also shown that the sensitivity of AGRP gene expression to loss of energy stores is maintained even when food intake has been voluntarily reduced in chickens during incubation and in response to a stressor. However, the similarity between birds and mammals does not appear to extend to the way AGRP and POMC gene expression are regulated. In particular, the preliminary evidence from the discovery of the first avian leptin (LEP) genes suggests that LEP is more pleiotropic in birds and may not even be involved in regulating energy balance. Similarly, ghrelin exerts inhibitory, rather than stimulatory, effects on food intake. The fact that the importance of these prominent long-term regulators of AGRP and POMC expression in mammals appears diminished in birds suggests that the balance of regulatory inputs in birds may have shifted to more short-term influences such as the tone of cholecystokinin (CCK) signalling. This is likely to be related to the different metabolic fuelling required to support flight.

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Fasting alters protein expression of AMP-activated protein kinase in the hypothalamus of broiler chicks (Gallus gallus domesticus)

Cited by 58 publications

References 57 publications

Glucocorticoids Increase NPY Gene Expression via Hypothalamic AMPK Signaling in Broiler Chicks

Glucocorticoids Increase NPY Gene Expression via Hypothalamic AMPK Signaling in Broiler Chicks

Peripheral Insulin Doesn’t Alter Appetite of Broiler Chicks

Regulation of the avian central melanocortin system and the role of leptin

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