Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy
Valentin Dunsing-Eichenauer,
Johan Hummert,
Claire Chardès
et al.
Abstract:Fluorescence lifetime imaging (FLIM) is widely used for functional and multiplexed bioimaging, but is limited in speed, volumetric sampling of multicellular specimen, and live cell compatibility. To overcome these limitations, we have combined single objective light-sheet microscopy with pulsed excitation and time-resolved detection on a SPAD array detector. We report excellent quantitative agreement with confocal FLIM at 10-100-fold shorter acquisition times, down to 100 ms per image. We demonstrate lifetime-… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.