Fast‐to‐slow shift of muscle fiber‐type composition by dietary apple polyphenols in rats: Impact of the low‐dose supplementation

Mizunoya, Wataru; Okamoto, Shinpei; Miyahara, Hideo; Akahoshi, Mariko; Suzuki, Takahiro; Q., Mai Khoi; Ohtsubo, Hideaki; Komiya, Yusuke; Qahar, Mulan; Waga, Toshiaki; Nakazato, Koichi; Ikeuchi, Yoshihide; Anderson, Judy E.; Tatsumi, Ryuichi

doi:10.1111/asj.12655

Cited by 19 publications

(13 citation statements)

References 46 publications

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“…Several lines of evidence have demonstrated that long-term endurance training induced an increased content of capillaries and mitochondria in skeletal muscle, promoted muscle fiber phenotype fast-to-slow (type II to typeI) transition, and thereby improved endurance capacity [25]. Recently, Mizunoya et al reported that apple polyphenols improved endurance by inducing fast-to-slow shift of muscle fiber type composition [26, 27]. We expect that myricetin, also a flavonoid, might promote muscle fiber type transition, which led to endurance enhancement.…”

Section: Discussionmentioning

confidence: 99%

Myricetin improves endurance capacity by inducing muscle fiber type conversion via miR-499

Ran

Zhou

et al. 2019

Nutr Metab (Lond)

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Background Reprogramming of fast-to-slow myofiber switch can improve endurance capacity and alleviate fatigue. Accumulating evidence suggests that a muscle-specific microRNA, miR-499 plays a crucial role in myofiber type transition. In this study, we assessed the effects of natural flavonoid myricetin on exercise endurance and muscle fiber constitution, and further investigated the underlying mechanism of myricetin in vivo and in vitro. Methods A total of 66 six-week-old male Sprague Dawley rats were divided into non-exercise or exercise groups with/without orally administered myricetin (50 or 150 mg/kg) for 2 or 4 weeks. Time-to-exhaustion, blood biochemical parameters, muscle fiber type proportion, the expression of muscle type decision related genes were measured. Mimic/ inhibitor of miR-499 were transfected into cultured L6 myotubes, the expressions of muscle type decision related genes and mitochondrial respiration capacity were investigated. Results Myricetin treatment significantly improved the time-to-exhaustion in trained rats. The enhancement of endurance capacity was associated with an increase of the proportion of slow-twitch myofiber in both soleus and gastrocnemius muscles. Importantly, myricetin treatment amplified the expression of miR-499 and suppressed the expression of Sox6, the down-stream target gene of miR-499, both in vivo and in vitro. Furthermore, inhibition of miR-499 overturned the effects of myricetin on down-regulating Sox6. Conclusions Myricetin promoted the reprogramming of fast-to-slow muscle fiber type switch and reinforced the exercise endurance capacity. The precise mechanisms responsible for the effects of myricetin are not resolved but likely involve regulating miR-499/Sox6 axis.

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Section: Discussionmentioning

confidence: 99%

Myricetin improves endurance capacity by inducing muscle fiber type conversion via miR-499

Ran

Zhou

et al. 2019

Nutr Metab (Lond)

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“…Many researchers have investigated various dietary supplements to inhibit muscle pathology or strengthen non-diseased muscles, whether it be for athletes or against aging, type 2 diabetes, or MD [ 83 , 84 , 85 , 86 , 87 , 110 , 128 , 129 , 130 , 131 , 132 , 133 ]. In MD, increasing the percentage of slow fibers is often the goal, but not necessarily the only goal.…”

Section: Dietary Supplementsmentioning

confidence: 99%

“…It has been demonstrated that a low dose (0.5% by weight) of apple polyphenols increased the percentage of slow, Type 2 fibers in plantari muscles (from ~48% to ~58%, p = 0.02), after feeding it to rats for 8 weeks [ 83 ]. As expected, this change correlated with fatigue resistance in the treated rats.…”

Section: Polyphenol Supplementmentioning

confidence: 99%

Skeletal Muscle Metabolism in Duchenne and Becker Muscular Dystrophy—Implications for Therapies

Heydemann

2018

Nutrients

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The interactions between nutrition and metabolism and skeletal muscle have long been known. Muscle is the major metabolic organ—it consumes more calories than other organs—and therefore, there is a clear need to discuss these interactions and provide some direction for future research areas regarding muscle pathologies. In addition, new experiments and manuscripts continually reveal additional highly intricate, reciprocal interactions between metabolism and muscle. These reciprocal interactions include exercise, age, sex, diet, and pathologies including atrophy, hypoxia, obesity, diabetes, and muscle myopathies. Central to this review are the metabolic changes that occur in the skeletal muscle cells of muscular dystrophy patients and mouse models. Many of these metabolic changes are pathogenic (inappropriate body mass changes, mitochondrial dysfunction, reduced adenosine triphosphate (ATP) levels, and increased Ca2+) and others are compensatory (increased phosphorylated AMP activated protein kinase (pAMPK), increased slow fiber numbers, and increased utrophin). Therefore, reversing or enhancing these changes with therapies will aid the patients. The multiple therapeutic targets to reverse or enhance the metabolic pathways will be discussed. Among the therapeutic targets are increasing pAMPK, utrophin, mitochondrial number and slow fiber characteristics, and inhibiting reactive oxygen species. Because new data reveals many additional intricate levels of interactions, new questions are rapidly arising. How does muscular dystrophy alter metabolism, and are the changes compensatory or pathogenic? How does metabolism affect muscular dystrophy? Of course, the most profound question is whether clinicians can therapeutically target nutrition and metabolism for muscular dystrophy patient benefit? Obtaining the answers to these questions will greatly aid patients with muscular dystrophy.

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“…In a recent study, we found that intake of certain food ingredients induced a transition in muscle fiber type in rat skeletal muscles. An 8‐wk supplementation with 0.5% apple polyphenols (APP) significantly up‐regulated slower MyHC isoforms in hind limb skeletal muscles of rats (Mizunoya et al., ), and supplementation with 5% APP increased skeletal muscle endurance capacity and altered muscle fiber type (increase of fibers expressing MyHC1; (Mizunoya et al., ). Furthermore, we reported that a transition from type 2B to type 2X fibers in extensor digitorum longus (EDL) muscles (MyHC2B was most abundant) was induced in rats fed fish oil compared with those fed soybean oil (Mizunoya et al., ).…”

Section: Introductionmentioning

confidence: 99%

Increase in muscle endurance in mice by dietary Yamabushitake mushroom (Hericium erinaceus) possibly via activation of PPARδ

Komiya

Nakamura

Ishii

et al. 2019

Animal Science Journal

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Skeletal muscle fiber is largely classified into two types: type 1 (slow‐twitch) and type 2 (fast‐twitch) fibers. Meat quality and composition of fiber types are thought to be closely related. Previous research showed that overexpression of constitutively active peroxisome proliferator‐activated receptor (PPAR)δ, a nuclear receptor present in skeletal muscle, increased type 1 fibers in mice. In this study, we found that hexane extracts of Yamabushitake mushroom (Hericium erinaceus) showed PPARδ agonistic activity in vitro. Eight‐week‐old C57BL/6J mice were fed a diet supplemented with 5% (w/w) freeze‐dried Yamabushitake mushroom for 24 hr. After the treatment period, the extensor digitorum longus (EDL) muscles were excised. The Yamabushitake‐supplemented diet up‐regulated the PPARδ target genes Pdk4 and Ucp3 in mouse skeletal muscles in vivo. Furthermore, feeding the Yamabushitake‐supplemented diet to mice for 8 weeks resulted in a significant increase in muscle endurance. These results indicate that Yamabushitake mushroom contains PPARδ agonistic ligands and that dietary intake of Yamabushitake mushroom could activate PPARδ in skeletal muscle of mice. Unexpectedly, we observed no significant alterations in composition of muscle fiber types between the mice fed control and Yamabushitake‐supplemented diets.

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Fast‐to‐slow shift of muscle fiber‐type composition by dietary apple polyphenols in rats: Impact of the low‐dose supplementation

Cited by 19 publications

References 46 publications

Myricetin improves endurance capacity by inducing muscle fiber type conversion via miR-499

Myricetin improves endurance capacity by inducing muscle fiber type conversion via miR-499

Skeletal Muscle Metabolism in Duchenne and Becker Muscular Dystrophy—Implications for Therapies

Increase in muscle endurance in mice by dietary Yamabushitake mushroom (Hericium erinaceus) possibly via activation of PPARδ

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