Fast supercritical fluid chromatography of polymers using packed capillary columns

Abstract

SummaryFast separations of perfluorinated polyethers and polymethylsiloxanes that are composed of 50-80 ohgomers were demonstrated in packed capillary column supercritical fluid chromatography (SFC) using a carbon dioxide mobile phase. Separations were accomplished within 10 min using a 13 cm x 250 I~m i.d. column packed with 2 I~m porous octadecyl bonded silica (ODS) particles. Effects of particle diameter of the packing material and pressure programming on separation were investigated, and packed column SFCwas compared with open tubular column SFC. Results show that as the particle diameter was decreased from 5 to 3 to 2 I~m and the column length was reduced from 85 to 43 to 13 cm, the separation time could be reduced from 70 to 20 to 10 min while still maintaining similar separation (resolution). Short colu mns packed with small porous particles are very suitable for fast SFC separations of polymers.

Supercritical Fluid and Unified Chromatography

“…Wu et al used packed-capillary-column SFC to separate perfluorinated polyethers and polymethylsiloxane oligomers with CO 2 mobile phase (34). Decreasing the particle size and the column length in concert, ultimately to 2 µm and 13 cm, respectively, led to a 7-fold reduction in analysis time while maintaining resolution between the peaks.…”

Fluidos supercríticos em química analítica. III.: aplicações

“…Neste caso, a cromatografia de permeação em gel (GPC, "gel permeation chromatography") é utilizada, mas não é tão precisa. Por esta razão, a cromatografia com fluido supercrítico é a única alternativa nestes casos, separando os oligômeros individuais e permitindo, inclusive, sua quantificação [49][50][51] .…”

Fast supercritical fluid chromatography hydrocarbon group-type separations of diesel fuels using packed and monolithic columns

“…SFC separation times can be further reduced using methods similar to those used to decrease separation times in HPLC, such as using shorter columns with smaller diameter packing. 5 This paper focuses on increasing the speed of a common industrial analysis performed by SFC-the separation of diesel fuels into hydrocarbon group-types (i.e., saturates, mono-, di-, tri-, and polyaromatics). Diesel and jet fuel environmental and performance properties have been associated with the group-type compositions of these fuels.…”

“…The first approach is to use shorter columns with smaller diameter packing. 5,[23][24][25][26][27] Shortening the column reduces the separation efficiency. Using a smaller diameter packing helps offset this by improving the plate height.…”