Fast start-up and enhancement of partial nitritation and anammox process for treating synthetic wastewater in a sequencing bath biofilm reactor: Strategy and function of nitric oxide

Huang, Ting; Zhao, Jian‐Qiang; Wang, Sha; Lei, Lin

doi:10.1016/j.biortech.2021.125225

Cited by 13 publications

(6 citation statements)

References 43 publications

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“…A previous study found that Acidobacteria could secrete EPS, in particular the PS, to defend themselves in an adverse environment (Shuai et al, 2021), and bacteria related to Proteobacteria could provide EPS and amino acids for species that produced signaling molecules (Guo, Zhao, et al, 2021). Moreover, a previous study reported that Armatimonadetes were crucial for preserving the integrity of biofilm aggregates in anammox systems (Huang et al, 2021). Also, compared with the floc sludge, the biofilm included a higher relative abundance of Verrucomicrobia (Zhang et al, 2015), and the majority of Parcubacteria congregated on the aggregates (Speth et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Extracellular polymeric substances and microbial community shift during the start‐up of a single‐stage partial nitritation/anammox process

Men,

Liu,

Wang

et al. 2023

Water Environment Research

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A sequencing batch reactor (SBR) was operated to investigate variations of extracellular polymeric substances (EPS) and microbial community during the start‐up of the single‐stage partial nitritation/anammox (SPN/A) process at intermittent aeration mode. The SPN/A system was successfully started on day 34, and the nitrogen removal efficiency and total nitrogen loading rate were 82.29% and 0.31 kg N/(m3·day), respectively. Furthermore, the relationship between the protein secondary structures and microbial aggregation was strongly related. The α‐helix/ (β‐sheet + random coil) ratios increased obviously from 0.20 ± 0.03 to 0.23 ± 0.01, with the sludge aggregation mean size increased from 56 to 107 μm during the start‐up of SPN/A. During the start‐up of SPN/A, Candidatus Kuenenia was the primary anammox bacteria, whereas Nitrospira was the main functional bacteria of nitrite‐oxidizing bacteria. Correlation between the microbial community and EPS components was performed. The EPS and microbial community played important roles in keeping stable nitrogen removal and the formation of sludge granules.Practitioner Points Intermittent aeration strategy promoted SPN/A system start‐up. EPS composition and protein secondary structure were related with the sludge disintegration and aggregation. Microbial community shift existed and promoted the stability of sludge and reactor performance during SPN/A start‐up.

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Section: Resultsmentioning

confidence: 99%

Extracellular polymeric substances and microbial community shift during the start‐up of a single‐stage partial nitritation/anammox process

Men,

Liu,

Wang

et al. 2023

Water Environment Research

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“…The source of nitrogen for the synthetic wastewater was NH 4 + -N (with the addition of NH 4 Cl) at a concentration of 110 ± 2.3 mg/L. Other components and trace elements in the synthetic wastewater were prepared according to [ 31 ]. The pH in the reactor was controlled at 7.5–8.23 by adding NaHCO 3 during continuous operation.…”

Section: Methodsmentioning

confidence: 99%

Surface Modification of Polyurethane Sponge with Zeolite and Zero-Valent Iron Promotes Short-Cut Nitrification

Liu,

Chen,

et al. 2024

Polymers

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Partial nitrification-Anammox (PN-A) is a cost-effective, environmentally friendly, and efficient method for removing ammonia (NH4+-N) pollutants from water. However, the limited accumulation of nitrite (NO2−-N) represents a bottleneck in the development of PN-A processes. To address this issue, this study developed a composite carrier loaded with nano zero-valent iron (nZVI) and zeolite to enhance NO2−-N accumulation during short-cut nitrification. The modified composite carrier revealed electropositive, hydrophilicity, and surface roughness. These surface characteristics correlate positively with the carrier’s total biomass adsorption capacity; the initial adsorption of microorganisms by the composite carrier was increased by 8.7 times. Zeolite endows the carrier with an NH4+-N adsorption capacity of 4.50 mg/g carrier. The entropy-driven ammonia adsorption process creates an ammonia-rich microenvironment on the surface of the carrier, providing effective inhibition of nitrite-oxidizing bacteria (NOB). In tests conducted with a moving bed biofilm reactor and a sequencing batch reactor, the composite carrier achieved a 95% NH4+-N removal efficiency, a NO2−-N accumulation efficiency of 78%, and a doubling in total nitrogen removal efficiency. This composite carrier enhances NO2−-N accumulation by preventing biomass washout, inhibiting NOB, and enriching PN-A functional bacteria, suggesting its potential for large-scale, stable PN-A applications.

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“…Besides a low DO concentration, several strategies regarding aeration control were applied to start-up the PN/A process. Huang et al [11] achieved start-up of the PN/A process in 30 days by implementing pre-and post-non-aeration. Another start-up was reached by gradually reducing the DO and regulating the nitrite [12].…”

Section: Introductionmentioning

confidence: 99%

“…Another method combining intensifying agitation and promoting the aeration rate successfully enhanced the PN/A process. NOB suppression was achieved by applying a special type of aeration control consisting of anaerobic-oxic-anoxic modes [11]. Pereira et al [14] reported aeration strategies of 30 and 45 min with anoxic periods in relation to 15 min aerobic periods, coupled with low air flow rates, which were better for PN/A establishment.…”

Section: Introductionmentioning

confidence: 99%

Decrease in Oxygen Concentration for the Fast Start-Up of Partial Nitritation/Anammox without Inoculum Addition

Borowska,

Zubrowska-Sudol,

Doskocz

2024

Sustainability

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Initiating the partial nitritation and anammox (PN/A) process without inoculation poses a significant challenge. Thus, there is a notable amount of interest in devising a straightforward strategy for the start-up of PN/A. This study demonstrates the feasibility of achieving the rapid start-up of a one-stage PN/A process within a moving-bed sequencing batch biofilm reactor (MBSBBR) by reducing the oxygen concentrations: 3.0 mg O2/L (Stage I), 2.0 mg O2/L (Stage II), and 1.0 mg O2/L (Stage III). The anammox activity was observed 15 days after a gradual decrease in the oxygen concentration and confirmed using a specific anammox activity test (5.9 mg N/gVSS∙h). During Stage III, the average total inorganic nitrogen (TIN) removal efficiency was 60.6%. The relative abundance of planctomycetes, a typical phylum representing anammox microorganisms, increased almost three times from Stage I (2.8%) to Stage III (7.1%). These results demonstrate the potential of a decrease in oxygen concentration for the fast start-up of the one-stage partial nitritation and anammox process without inoculation. The implementation of the studied DO strategy has practical implications for wastewater treatment plant operators, particularly in the start-up of the PN/A processes. Additionally, batch assays allow for the rapid assessment of treatment plant performance, providing real-time insights into its functionality and, thereby, optimizing wastewater treatment practices.

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Fast start-up and enhancement of partial nitritation and anammox process for treating synthetic wastewater in a sequencing bath biofilm reactor: Strategy and function of nitric oxide

Cited by 13 publications

References 43 publications

Extracellular polymeric substances and microbial community shift during the start‐up of a single‐stage partial nitritation/anammox process

Extracellular polymeric substances and microbial community shift during the start‐up of a single‐stage partial nitritation/anammox process

Surface Modification of Polyurethane Sponge with Zeolite and Zero-Valent Iron Promotes Short-Cut Nitrification

Decrease in Oxygen Concentration for the Fast Start-Up of Partial Nitritation/Anammox without Inoculum Addition

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