Fast solid-phase extraction–gas chromatography–mass spectrometry procedure for oil fingerprinting

Alzaga, Roberto; Montuori, Paolo; Ortiz, Laura; Bayona, Josep M.; Albaigés, Joan

doi:10.1016/j.chroma.2003.10.080

Cited by 86 publications

(40 citation statements)

References 20 publications

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“…Ox39 with 2MN as the only carbon source were unsuccessful, but there were strong indications that this strain could cometabolize dimethylnaphthalenes when m-xylene was the major organic substrate (57). The main contaminants in the Prestigeaffected sediments were naphthalene and alkylated derivatives (see Tables S2a and S2b in the supplemental material) (41,58), including primarily methylated and dimethylated compounds. We thus associated the Atlantic Islands sequences in this cluster with the degradation of methylated naphthalenes.…”

Section: Resultsmentioning

confidence: 99%

Diversity of Benzylsuccinate Synthase-Like ( bssA ) Genes in Hydrocarbon-Polluted Marine Sediments Suggests Substrate-Dependent Clustering

Acosta-González

Rosselló‐Móra

Marqués

2013

Appl Environ Microbiol

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bThe potential of hydrocarbon biodegradation in marine sediments was determined through the detection of a functional biomarker, the bssA gene, coding for benzylsuccinate synthase, the key enzyme of anaerobic toluene degradation. Eight bssA clone libraries (409 sequences) were constructed from polluted sediments affected by the Prestige oil spill in the Atlantic Islands National Park and from hydrocarbon-amended sediment microcosms in Mallorca. The amplified products and database-derived bssA-like sequences grouped into four major clusters, as determined by phylogenetic reconstruction, principal coordinate analysis (PCoA), and a subfamily prediction tool. In addition to the classical bssA sequences that were targeted, we were able to detect sequences homologous to the naphthylmethylsuccinate synthase gene (nmsA) and the alkylsuccinate synthase gene (assA), the bssA homologues for anaerobic 2-methylnaphthalene and alkane degradation, respectively. The detection of bssA-like variants was determined by the persistence and level of pollution in the marine samples. The observed level of gene diversity was lower in the Mallorca sediments, which were dominated by assA-like sequences. In contrast, the Atlantic Islands samples, which were highly contaminated with methylnaphthalene-rich crude oil, showed a high proportion of nmsA-like sequences. Some of the detected genes were phylogenetically related to Deltaproteobacteria communities, previously described as the predominant hydrocarbon degraders at these sites. Differences between all detected bssA-like genes described to date indicate separation between marine and terrestrial sequences and further subgrouping according to taxonomic affiliation. Global analysis suggested that bssA homologues appeared to cluster according to substrate specificity. We observed undetected divergent gene lineages of bssA homologues, which evidence the existence of new degrader groups in these environments.

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Section: Resultsmentioning

confidence: 99%

Diversity of Benzylsuccinate Synthase-Like ( bssA ) Genes in Hydrocarbon-Polluted Marine Sediments Suggests Substrate-Dependent Clustering

Acosta-González

Rosselló‐Móra

Marqués

2013

Appl Environ Microbiol

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“…Fractionation: The extracts were fractionated using 6 ml polypropylene cartridges (Interchim) containing 1 g silica and 0.5 g cyanopropyl-silica (Alzaga et al 2004). We collected 2 fractions, (1) aliphatic hydrocarbons eluting with 4 ml of hexane and (2) polycyclic aromatic hydrocarbons with 8 ml of hexane:dichloro- NW Spain, where mussel seed were collected methane (1:1).…”

Section: Methodsmentioning

confidence: 99%

Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach

Labarta

Fernández‐Reiriz²,

Garrido³

et al. 2005

Mar. Ecol. Prog. Ser.

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Postlarval recruits of mussels Mytilus galloprovincialis were sampled in February 2003 at 7 localities along the Galicia coast (NW Spain) exhibiting different levels of impact from the 'Prestige' oil spill. These localities are important grounds for seed collection used in raft culture. The concentrations of polycyclic aromatic hydrocarbons (PAHs) as well as different biochemical and ecophysiological variables were determined in mussel tissues, as indicators of the toxic impact of the spilled oil. The following parameters were measured: size frequency distributions and size-weight relationships, survival in air, biochemical composition (proteins, carbohydrates, glycogen and lipids), lipid classes (phospholipids, sterol esters and waxes, triacylglycerols, free fatty acids and sterols), and fatty acid composition. The study determined sub-lethal effects in wild mussel seed populations sampled 3 mo after the oil spill. These effects are shown in the form of survival indices and alterations of lipid metabolism, especially in trygliceride and sterol fractions, as well as in saturated/monounsaturated fatty acid ratios. Monitoring these effects in wild mussel recruits is important for analysing growth, production and biochemical reserve cycle throughout the raft cultivation period and evaluating the capacity of the individuals to repair alterations detected in the juvenile stage. KEY WORDS: Mussel · Postlarvae · 'Prestige' · Toxic impact · PAH · Biochemical analysisResale or republication not permitted without written consent of the publisher

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“…The Costa da Morte, northwestern Spain, was the most affected area (2). The oil residue released by the Prestige was devoid of the more labile fractions (boiling point, Ͻ300°C), with high levels of aromatic hydrocarbons (ϳ50%), as well as resins and asphaltenes (ϳ30%) (6).…”

mentioning

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Bacterial Communities from Shoreline Environments (Costa da Morte, Northwestern Spain) Affected by the Prestige Oil Spill

Alonso-Gutierrez

Figueras

Albaigés

et al. 2009

Appl Environ Microbiol

131

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The bacterial communities in two different shoreline matrices, rocks and sand, from the Costa da Morte, northwestern Spain, were investigated 12 months after being affected by the Prestige oil spill. Culture-based and culture-independent approaches were used to compare the bacterial diversity present in these environments with that at a nonoiled site. A long-term effect of fuel on the microbial communities in the oiled sand and rock was suggested by the higher proportion of alkane and polyaromatic hydrocarbon (PAH) degraders and the differences in denaturing gradient gel electrophoresis patterns compared with those of the reference site. Members of the classes Alphaproteobacteria and Actinobacteria were the prevailing groups of bacteria detected in both matrices, although the sand bacterial community exhibited higher species richness than the rock bacterial community did. Culture-dependent and -independent approaches suggested that the genus Rhodococcus could play a key role in the in situ degradation of the alkane fraction of the Prestige fuel together with other members of the suborder Corynebacterineae. Moreover, other members of this suborder, such as Mycobacterium spp., together with Sphingomonadaceae bacteria (mainly Lutibacterium anuloederans), were related as well to the degradation of the aromatic fraction of the Prestige fuel. The multiapproach methodology applied in the present study allowed us to assess the complexity of autochthonous microbial communities related to the degradation of heavy fuel from the Prestige and to isolate some of their components for a further physiological study. Since several Corynebacterineae members related to the degradation of alkanes and PAHs were frequently detected in this and other supralittoral environments affected by the Prestige oil spill along the northwestern Spanish coast, the addition of mycolic acids to bioremediation amendments is proposed to favor the presence of these degraders in long-term fuel pollution-affected areas with similar characteristics.

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Fast solid-phase extraction–gas chromatography–mass spectrometry procedure for oil fingerprinting

Cited by 86 publications

References 20 publications

Diversity of Benzylsuccinate Synthase-Like ( bssA ) Genes in Hydrocarbon-Polluted Marine Sediments Suggests Substrate-Dependent Clustering

Diversity of Benzylsuccinate Synthase-Like ( bssA ) Genes in Hydrocarbon-Polluted Marine Sediments Suggests Substrate-Dependent Clustering

Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach

Bacterial Communities from Shoreline Environments (Costa da Morte, Northwestern Spain) Affected by the Prestige Oil Spill

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Fast solid-phase extraction–gas chromatography–mass spectrometry procedure for oil fingerprinting

Cited by 86 publications

References 20 publications

Diversity of Benzylsuccinate Synthase-Like ( bssA ) Genes in Hydrocarbon-Polluted Marine Sediments Suggests Substrate-Dependent Clustering

Diversity of Benzylsuccinate Synthase-Like ( bssA ) Genes in Hydrocarbon-Polluted Marine Sediments Suggests Substrate-Dependent Clustering

Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach

Bacterial Communities from Shoreline Environments (Costa da Morte, Northwestern Spain) Affected by the Prestige Oil Spill

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Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach