Fast simultaneous quantitation of valsartan and amlodipine besylate using an eco-friendly micellar HPLC-UV method: application to spiked human plasma and content uniformity testing for amlodipine

Ibrahim, F.; El-Brashy, A.; El-Awady, Mohamed I.; Abdallah, Nora A.

doi:10.1039/c8ay01263f

Cited by 8 publications

(2 citation statements)

References 14 publications

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“…The validation of a dynamic quenching process was establishing by the ultraviolet (UV) spectra of basic (Ibrahim, et al, 2018) HPLC-UV 8.01 24.2 (Shaikh, et al, 2020) HPLC-UV 0.053 0.16 (Marghany, et al, 2020) UV Spectrophotometry 0.51 1.70 (Satana, et al, 2001) Ratio derivative spectrophotometry 0.628 2.09 (Dinc, Uslu, and Özkan 2004) Spectrofluorimetric 0.40 0.50 (Cagigal, et al, 2001…”

Section: J Application Of the Fluorescence Quenching Methodsmentioning

confidence: 99%

New Fluorescence Quenching Approach for Determination of Valsartan in Certain Tablets and Spiked Biological Fluids

Ali

2020

ARO

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A new, simple, selective, sensitive, fast, economical, and reliable fluorescence quenching method for the quantitation of valsartan was investigated using basic fuchsin act as a fluorometric dye. The method was depended on the detection quenching influence of valsartan on the fluorescence intensity of basic fuchsin and the reaction between valsartan and basic fuchsin in a McIlvaine buffer medium at pH = 6 to yield a new basic fuchsin–valsartan non-fluorescent complex. The excitation and emission of basic fuchsin fluorescence signal were identified at 535 and 728 nm, respectively. A fluorescence quenching value (ΔF) displayed a very good linear relationship (R2 = 0.9992) with valsartan concentration ranging from 0.003 to 3 μg/mL, a detection limit as low as 0.0009 μg/mL with a high precision and accuracy (RSD% <3). Significantly, no interference effect was found due to the presence of other ingredients commonly found in medical formulations. The acquired data were statistically compared with those acquired from reported chromatographic method and were observed to be in excellent agreement at a 95% confidence level; the planned fluorescence quenching procedure was subsequently utilized to detected the concentration of valsartan in spiked biological fluids and commercial medical tablets.

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Section: J Application Of the Fluorescence Quenching Methodsmentioning

confidence: 99%

New Fluorescence Quenching Approach for Determination of Valsartan in Certain Tablets and Spiked Biological Fluids

Ali

2020

ARO

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“…18 For VAL, UV spectrophotometry, [19][20][21][22] HPLC, 19,23 and HPTLC 24 methods were reported. Published methods for simultaneous determination of AMD and VAL include UV spectrophotometric, [25][26][27][28][29][30][31][32][33][34][35][36] spectrofluorimetric methods [37][38][39] , HPLC, 25,30,31,[40][41][42][43][44][45][46][47] TLC 44,48 and capillary electrophoresis. 49,50 The present research work is concerned with the development of two simple, accurate and sensitive spectrophotometric methods: absorption correction method (ACM) and dual wavelength method (DWM) for simultaneous determination of AMD and VAL with no need for prior separation.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Determination of Amlodipine and Valsartan in Pharmaceutical Dosage Form

El‐Malla

Hammad

Khashaba

2021

Journal of Advanced Medical and Pharmaceutical Research

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Two simple spectrophotometric methods had been developed for simultaneous determination of amlodipine (AMD) and valsartan (VAL) in their tablet dosage form. These methods include absorption correction method and dual wavelength method. The first method was based on the measurement of absorbance at 360 nm for AMD and 257.1 nm for VAL, while the second depended on measuring the absorbance difference (A) in zero order spectra between (226.5-250 nm) for determination of VAL while AMD was determined directly by measuring absorbance at 360 nm. Both methods were validated according to International Council for Harmonisation (ICH) guidelines. The linearity range was found to be 8-25 μg/mL for determination of AMD and 6-35 μg/mL for determination of VAL. The methods were successfully applied for the simultaneous determination of AMD and VAL in the commercially available Exforge ® tablets. The average values of percent recovery  standard deviation was found to be consistent with the label claim of the dosage form. The results were also compared to a reported method using t-test and F-test at confidence level of 95%, no significant differences were observed. The presented methods permit simple, rapid, and direct determination of AMD and VAL in commercially available combined dosage form using zero-order UV spectra without previous separations and were suitable for routine analysis.

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Simultaneous determination of hydrochlorothiazide, amlodipine, and telmisartan with spectrophotometric and HPLC green chemistry applications

et al. 2022

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Fast simultaneous quantitation of valsartan and amlodipine besylate using an eco-friendly micellar HPLC-UV method: application to spiked human plasma and content uniformity testing for amlodipine

Abstract: A simple, sensitive, and economic quantitation of two co-formulated antihypertensive drugs, valsartan and amlodipine besylate was carried out in less than 4 minutes.

Cited by 8 publications

References 14 publications

New Fluorescence Quenching Approach for Determination of Valsartan in Certain Tablets and Spiked Biological Fluids

New Fluorescence Quenching Approach for Determination of Valsartan in Certain Tablets and Spiked Biological Fluids

Quantitative Determination of Amlodipine and Valsartan in Pharmaceutical Dosage Form

Simultaneous determination of hydrochlorothiazide, amlodipine, and telmisartan with spectrophotometric and HPLC green chemistry applications

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