Fast simultaneous determination of 14 nucleosides and nucleobases in cultured Cordyceps using ultra-performance liquid chromatography

Yang, Feng‐Qing; Guan, Jin; Li, S.P.

doi:10.1016/j.talanta.2007.03.034

Cited by 138 publications

(81 citation statements)

References 21 publications

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“…Actually, many works for the qualitative and quantitative determination of nucleosides and their bases in Cordyceps have been reported. Several methods, including HPLC-DAD (diode array detector) [4,5], CE [6], CEC [7], LC-MS [8], ultra performance liquid chromatography [9], have been used for nucleosides analysis in Cordyceps. Sterols, especially ergosterol, have also been determined using HPLC-DAD [5], LC-MS [10] and GC-MS [11].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by HPLC coupled with diode array detection and evaporative light scattering detection

Wang

Yang

Feng

et al. 2009

J of Separation Science

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A HPLC coupled with diode array detection (DAD) and evaporative light scattering detection (ELSD) method for qualitative and quantitative analysis of eight nucleosides and nucleobases, three carbohydrates and myriocin in Cordyceps was developed. A Prevail Carbohydrate ES column was employed for the separation within 50 min. Nucleosides and their bases were tested at UV 254 nm. ELSD was connected with DAD to determine myriocin and carbohydrates. The optimum drift tube temperature of ELSD was at 941C with the nitrogen flow rate of 2.0 L/min. All calibration curves showed good linearity (R 2 40.9933) during the test ranges. The precision, repeatability, accuracy, LOD and LOQ were also fully investigated. This developed method was successfully applied to quantify 12 components, eight nucleosides and nucleobases, three carbohydrates and myriocin, in natural and cultured Cordyceps, which provides another view for quality control of Cordyceps sinensis.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by HPLC coupled with diode array detection and evaporative light scattering detection

Wang

Yang

Feng

et al. 2009

J of Separation Science

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“…The profile of nucleosides, nucleobases and uniquely modified nucleosides, instead of any single metabolite, can be feasible for the quality control of Cordyceps sensu lato products (Shiao et al 1994; Xiao and Xiong 2013). The nucleoside profile of Ophiocordyceps species and its allies has been explored, and the analytical methods of determining nucleosides and nucleobases in Cordyceps have been reported previously (Li et al 2004; Fan et al 2006; Yang et al 2007, 2009; Xiao and Xiong 2013; Zhang et al 2014; Qian and Li 2017). High performance liquid chromatography (HPLC), combined with ultraviolet (UV), evaporative light scattering detector or mass spectra, has been applied to analyse the profile of nucleosides, nucleobases in Cordyceps -related species, and proved to be a simple, convenient and reliable method.…”

Section: Introductionmentioning

confidence: 99%

Determination and comparative analysis of 13 nucleosides and nucleobases in natural fruiting body of Ophiocordyceps sinensis and its substitutes

et al. 2017

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Nucleosides and nucleobases are one of the most important indicators of quality control. A sensitive and reliable high performance liquid chromatography-ultraviolet method was applied to analyse 13 nucleosides and nucleobases simultaneously in 15 batches of nine Ophiocordyceps species and its allies in China. Principal component analysis (PCA) and cluster analysis were conducted by SPSS 22.0 software (IBM Corp., Armonk, NY, USA). The 15 samples of Cordyceps were differentiated successfully based on their nucleoside and nucleobase content. Total nucleosides content in mycelium was significantly higher than that in the natural fruiting bodies of Ophiocordyceps sinensis (NFOS). Five nucleosides or nucleobases – adenine (A), guanosine (Gu), uracil (U), uridine (Ur) and guanine (G) – were the major components contributed to the total variance according to PCA. The profiles of the 13 tested nucleosides and nucleobases (including adenosine, cytidine, guanosine, inosine, thymidine, uridine, cordycepin, adenine, cytosine, guanine, thymine, uracil and hypoxanthine) can discriminate different samples and can be candidate indicators applied for the quality control of Ophiocordyceps and its allies.

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“…Lots of analytical methods for determination of hypoxanthine were reported, such as hydrophilicinteraction chromatography (HILIC), 9 highperformance liquid chromatography coupled with diode array detection and evaporative light scattering detection (HPLC-DAD-ELSD), 10 high performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS), 11 ultra-performance liquid chromatography (UPLC), 12 capillary electrophoresis-mass spectrometry (CE-MS) 13 and capillary electrochromatography (CEC).…”

Section: Introductionmentioning

confidence: 99%

Improvement of NIR models for quality parameters of leech and earthworm medicines using outlier multiple diagnoses

Chen

et al. 2017

J. Innov. Opt. Health Sci.

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Leeches and earthworms are the main ingredients of Shuxuetong injection compositions, which are natural biomedicines. Near infrared (NIR) di®use re°ection spectroscopy has been used for quality assurance of Chinese medicines. In the present work, NIR spectroscopy was proposed as a rapid and nondestructive technique to assess the moisture content (MC), soluble solid content (SSC) and hypoxanthine content (HXC) of leeches and earthworms. This study goal was to improve NIR models for accurate quality control of leech and earthworm using outlier multiple diagnoses (OMD). OMD was composed of four outlier detection methods: spectrum outlier diagnostic (MD), leverage diagnostic (LD), principal component scores diagnostic (PCSD) and factor loading diagnostic (FLD). Conventional outlier diagnoses (MD, LD) and OMD were compared, and the best NIR models were those based on OMD. The correlation coe±cients (R) for leech were 0.9779, 0.9616 and 0.9406 for MC, SSC and HXC, respectively. The values of relative standard error of prediction (RSEP) for leech were 2.3%, 5.1% and 9.0% for MC, SSC and HXC, respectively. The values of R for earthworm were 0.9478, 0.9991 and 0.9605 for MC, SSC and HXC, respectively. The values of RSEP for earthworm were 8.8%, 2.4% and 12% for MC, SSC and HXC, respectively. The performance of the NIR models was certainly improved by OMD.

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Fast simultaneous determination of 14 nucleosides and nucleobases in cultured Cordyceps using ultra-performance liquid chromatography

Cited by 138 publications

References 21 publications

Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by HPLC coupled with diode array detection and evaporative light scattering detection

Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by HPLC coupled with diode array detection and evaporative light scattering detection

Determination and comparative analysis of 13 nucleosides and nucleobases in natural fruiting body of Ophiocordyceps sinensis and its substitutes

Improvement of NIR models for quality parameters of leech and earthworm medicines using outlier multiple diagnoses

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