Fast screening of tyrosinase inhibitors from traditional Chinese medicinal plants by ligand fishing in combination with in situ fluorescent assay

Yan, Zhao; Hu, Jie; Bai, Xiao-Bo; Liu, Hao-Peng; Qi, Xu-Wei; Liao, Xun

doi:10.1007/s00216-021-03864-w

Cited by 12 publications

(7 citation statements)

References 38 publications

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“…These characteristic conserves the colloidal stability of nano-sized MPs since they do not present remaining magnetization after the removal of the external field, theoretically avoiding agglomeration [22]. Beyond superparamagnetic properties, the surface functionalization of MPs confers versatile chemical features to this material that are responsible for its wide range of applications [23][24][25][26]. Figure 5 shows the general procedure for ligand fishing using magnetic particles.…”

Section: Magnetic Particlesmentioning

confidence: 99%

Ligand fishing as a tool to screen natural products with anticancer potential

Filho

Oliveira

Leal

et al. 2023

J of Separation Science

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Cancer is the second leading cause of death in the world and its incidence is expected to increase with the aging of the world's population and globalization of risk factors. Natural products and their derivatives have provided a significant number of approved anticancer drugs and the development of robust and selective screening assays for the identification of lead anticancer natural products are essential in the challenge of developing personalized targeted therapies tailored to the genetic and molecular characteristics of tumors. To this end, a ligand fishing assay is a remarkable tool to rapidly and rigorously screen complex matrices, such as plant extracts, for the isolation and identification of specific ligands that bind to relevant pharmacological targets. In this paper, we review the application of ligand fishing with cancer‐related targets to screen natural product extracts for the isolation and identification of selective ligands. We provide critical analysis of the system configurations, targets, and key phytochemical classes related to the field of anticancer research. Based on the data collected, ligand fishing emerges as a robust and powerful screening system for the rapid discovery of new anticancer drugs from natural resources. It is currently an underexplored strategy according to its considerable potential.

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Section: Magnetic Particlesmentioning

confidence: 99%

Ligand fishing as a tool to screen natural products with anticancer potential

Filho

Oliveira

Leal

et al. 2023

J of Separation Science

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“…Our group have reported the selective extraction of pancreatic lipase ligands from oolong tea and tyrosinase inhibitors from Scutellaria baicalensis Georgi. (Lamiaceae) and Sophora flavescens Aiton (Fabaceae) by MNPs immobilised with the respective enzymes 20,21 …”

Section: Introductionmentioning

confidence: 99%

“…(Lamiaceae) and Sophora flavescens Aiton (Fabaceae) by MNPs immobilised with the respective enzymes. 20,21 In this work, by using MAO-B-immobilised MNPs (MNPs@MAO-B) as fishing bait, we screened and identified the ligands of the enzyme from extracts of P. grandiflora roots by combination of HPLC-MS and nuclear magnetic resonance (NMR). The inhibitory activities as well as enzymatic kinetics of the MAO-B inhibitors were studied, and their molecular docking were simulated.…”

mentioning

confidence: 99%

Ligand fishing of monoamine oxidase B inhibitors from Platycodon grandiflorus (Jacq.) A.DC. roots by the enzyme functionalised magnetic nanoparticles

Yuan

Bai

et al. 2022

Phytochemical Analysis

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Introduction: As a famous traditional Chinese medicine, roots of Platycodon grandiflorus (Jacq.) A.DC. have shown multiple effects against neurodegenerative diseases. To investigate the components against Parkinson's disease (PD), the roots of P. grandiflora were selected as the research subject.Objective: Screening and identifying of monoamine oxidase B (MAO-B) inhibitors from the roots of P. grandiflorum via enzyme functionalised magnetic nanoparticles (MNPs)-based ligand fishing combined with high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis.Method: MAO-B functionalised MNPs have been synthesised for screening MAO-B inhibitors from the roots of P. grandiflorum. The ligands were identified by HPLC-MS and nuclear magnetic resonance (NMR) analysis, and their anti-PD activity was evaluated via MAO-B inhibition assay and cell viability assay in vitro.Results: Two MAO-B inhibitors were fished out and identified by HPLC-MS as protocatechuic aldehyde (1) and coumarin (2), with the half maximal inhibitory concentrations of 28.54 ± 0.39 and 25.39 ± 0.29 μM, respectively. Among them, 1 could also significantly increase the viability of 6-hydroxydopamine-damaged PC12 cells. Conclusion:The results are helpful to elucidate the anti-PD activity of the plant, and the ligand fishing method has shown good potential in discovery of MAO-B inhibitors.

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“…Due to the excess sun exposure, inflammation, or hormonal changes, more melanin is produced which results in dark patches and uneven pigmentation on the skin. Tyrosinase inhibitor would be able to block melanin biosynthesis and be important for medicine development to treat the hypopigmentation‐related disease (Gür et al, 2021, Zhao et al, 2022; Wu et al, 2015). To date, many tyrosinase inhibitors have been reported from food materials.…”

Section: Introductionmentioning

confidence: 99%