Fast screening of polycyclic aromatic hydrocarbons using trapped ion mobility spectrometry – mass spectrometry

Castellanos, Anthony; Benigni, Paolo; Hernández, Diana R.; DeBord, John Daniel; Ridgeway, Mark E.; Park, M. A.; Fernandez-Lima, Francisco

doi:10.1039/c4ay01655f

Cited by 51 publications

(66 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[118–121] In addition, changes in the conformational space can be observed as a function of the desolvation process and upon activation prior to the IMS-MS measurements. Previous work using TIMS-MS has shown the possibility to trap a wide mobility range from small molecules to small proteins[122, 123]; however, it was also demonstrated that the trapping efficiency is a function of the electrode geometry, bath gas profile and electrical confining parameters (e.g., radiofrequency value and amplitude).…”

Section: Resultsmentioning

confidence: 99%

Towards the analysis of high molecular weight proteins and protein complexes using TIMS-MS

Benigni

Marin

Molano-Arévalo

et al. 2016

Int. J. Ion Mobil. Spec.

View full text Add to dashboard Cite

In the present work, we demonstrate the potential and versatility of TIMS for the analysis of proteins, DNA-protein complexes and protein-protein complexes in their native and denatured states. In addition, we show that accurate CCS measurement are possible and in good agreement with previously reported CCS values using other IMS analyzers (<5% difference). The main challenges for the analysis of high mass proteins and protein complexes in the mobility and m/z domain are described. That is, the analysis of high molecular weight systems in their native state may require the use of higher electric fields or a compromise in the TIMS mobility resolution by reducing the bath gas velocity in order to effectively trap at lower electric fields. This is the first report of CCS measurements of high molecular weight biomolecules and biomolecular complexes (~ 150 kDa) using TIMS-MS.

show abstract

Section: Resultsmentioning

confidence: 99%

Towards the analysis of high molecular weight proteins and protein complexes using TIMS-MS

Benigni

Marin

Molano-Arévalo

et al. 2016

Int. J. Ion Mobil. Spec.

View full text Add to dashboard Cite

show abstract

“…A significant number of the small molecules CCS values consists of aromatic hydrocarbons. 98,119,139,162–164 Starting in the 1990s, interest in inorganic compounds (metal salts, atomic and molecular clusters) began to emerge. Very few inorganic compound CCS values were reported between 2000 and 2010, with a resurgence of interest starting in 2013 which were primarily focused on gaining fundamental insights into the structures of inorganic salt and metal clusters.…”

Section: Ccs Coverage Over Timementioning

confidence: 99%

Ion Mobility Collision Cross Section Compendium

2016

View full text Add to dashboard Cite

In this review, we focus on an important aspect of ion mobility (IM) research, namely the reporting of quantitative ion mobility measurements in the form of the gas-phase collision cross section (CCS), which has provided a common basis for comparison across different instrument platforms and offers a unique form of structural information, namely size and shape preferences of analytes in the absence of bulk solvent. This review surveys the over 24,000 CCS values reported from IM methods spanning the era between 1975 to 2015, which provides both a historical and analytical context for the contributions made thus far, as well as insight into the future directions that quantitative ion mobility measurements will have in the analytical sciences. The analysis was conducted in 2016, so CCS values reported in that year are purposely omitted. In another few years, a review of this scope will be intractable, as the number of CCS values which will be reported in the next three to five years is expected to exceed the total amount currently published in the literature.

show abstract

“…9 Photoionization has been used for the analysis of chemical warfare agents with standalone ion mobility spectrometers, mainly as a non-harmful and "soft" replacement for the radioactive 63 Ni source. 23 18,27 Yet to date no studies are published that take advantage of an APPI/APLI-IMS-FTMS setup.…”

Section: Introductionmentioning

confidence: 95%

1- and 2-Photon Ionization for Online FAIMS-FTMS Coupling Allows New Insights into the Constitution of Crude Oils

Vetere

Schräder

2015

Anal. Chem.

View full text Add to dashboard Cite

Photoionization techniques (APPI and APLI) are important for the mass spectrometric analysis of crude oils, given the mainly unpolar character of the sample. Ultrahigh resolving Fourier Transform mass spectrometry (FTMS) allows to distinguish between most isobaric compounds as well as to unambiguously determine the elemental compositions of the detected ions. Nevertheless, the complexity of crude oil makes its thorough analysis a difficult task. Besides discriminating effects that can be avoided and depth of information that can be gained by simplification of the sample prior to the MS analysis the presence of numerous isomeric compounds limits the amount of information that can be gained by mass spectrometry alone. Ion mobility spectrometry (IMS) has been shown to be a valuable tool for isomer separation and has also been employed for the analysis of crude oils using IMS-TOF MS. The application of an online FAIMS-FTMS coupling after photoionization for the analysis of crude oils is shown. With this setup the complementarity of data obtained from both APPI and APLI ionization is demonstrated. Online separation and individual detection of different hydrocarbon isomers is achieved.

show abstract

Fast screening of polycyclic aromatic hydrocarbons using trapped ion mobility spectrometry – mass spectrometry

Cited by 51 publications

References 27 publications

Towards the analysis of high molecular weight proteins and protein complexes using TIMS-MS

Towards the analysis of high molecular weight proteins and protein complexes using TIMS-MS

Ion Mobility Collision Cross Section Compendium

1- and 2-Photon Ionization for Online FAIMS-FTMS Coupling Allows New Insights into the Constitution of Crude Oils

Contact Info

Product

Resources

About