Fast screening method for the profile analysis of polycyclic aromatic hydrocarbon metabolites in urine using derivatisation–solid-phase microextraction

Gmeiner, Günter; Krassnig, C; Schmid, E.; Tausch, H.

doi:10.1016/s0378-4347(97)00526-4

Cited by 74 publications

(38 citation statements)

References 17 publications

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“…1-OHP is a urinary PAH metabolite that has often been used as a biomarker for recent exposure to multiple routes of PAHs. 1-OHP in human urine has been measured with analytical equipments like HPLC with fluorescence detection (HPLC-FL), GC-MS, LC-MS/MS [11][12][13][14][15][16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

“…The hydrolysis step is time-consuming, and its possible incomplete hydrolysis should be taken into consideration [8]. GC-MS methods and some LC-MS/MS methods require a derivatization step in addition to the hydrolysis [15,17,20,21]. Thus, direct measurement of 1-hydroxypyrene-glucuronide (1-OHP-G) would be ideal for the analysis of urine samples.…”

Section: Introductionmentioning

confidence: 99%

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Direct measurement of the glucuronide conjugate of 1-hydroxypyrene in human urine by using liquid chromatography with tandem mass spectrometry

Kakimoto

Toriba

Ohno

et al. 2008

Journal of Chromatography B

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To evaluate human exposure to polycyclic aromatic hydrocarbons (PAHs), we developed a rapid, simple and sensitive method for determining 1-hydroxypyrene-glucuronide (1-OHP-G) in human urine. To improve precision, a deuterated glucuronide was used as an internal standard. The method requires only 1 mL of urine. The urine was treated with a mixed-mode anion-exchange and reversed-phase solid-phase extraction cartridge (Oasis MAX). The analytes were analyzed with a C 18 reversed-phase column with a gradient elution, followed by tandem mass spectrometry with electrospray ionization in negative ion mode. The detection limit of 1-OHP-G (corresponding to a signal-to-noise ratio of 3) was 0.13 fmol/injection. Urinary concentrations of 1-OHP-G determined by this method were strongly correlated (r 2 =0.961) with concentrations of 1-hydroxypyrene by conventional HPLC with fluorescence detection.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Direct measurement of the glucuronide conjugate of 1-hydroxypyrene in human urine by using liquid chromatography with tandem mass spectrometry

Kakimoto

Toriba

Ohno

et al. 2008

Journal of Chromatography B

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“…There are, however, also instances when the LC route cannot be used and SPME-cum-derivatization has to be applied [235]. Derivatization can be performed in different ways, with direct derivatization in the sample matrix [236] and on the fibre [237] being most popular. Derivatization in the GC injection port is also used [238].…”

Section: Solid-phase Micro-extractionmentioning

confidence: 99%

“…Finally, the on-fibre alternative was applied for, e.g., PAH metabolites in urine [237]. SPME with an 85-lm polyacrylate fibre was rather timeconsuming, i.e., 45 min at 35°C under stirring.…”

Section: Solid-phase Micro-extractionmentioning

confidence: 99%

Modern Methods of Sample Preparation for GC Analysis

Koning¹,

Janssen

Brinkman

2009

Chroma

121

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Today, a wide variety of techniques is available for the preparation of (semi-) solid, liquid and gaseous samples, prior to their instrumental analysis by means of capillary gas chromatography (GC) or, increasingly, comprehensive two-dimensional GC (GC 9 GC). In the past two decades, a large number of 'modern' sample-preparation techniques has been introduced, which have partly superseded their 'classical' counterparts. These novel techniques include off-line and on-line (sometimes semi-or fully automated) procedures, and exhaustive extraction as well as equilibrium techniques. In order to improve overall performance, aspects such as essentially organic solvent-less approaches, large-volume injection and miniaturization receive increasing attention. In most recent applications, mass spectrometric or element-selective detection have been used. The present review discusses the advantages and disadvantages, and relative performance, of most of the modern samplepreparation techniques and cites a number of illustrative applications for each of them.

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“…16 The SPME method has subsequently been applied to detect trace organic micro-pollutants, such as volatile organic compounds (VOCs), PAHs and biological samples. [17][18][19] Moreover, when SPME is used for biological samples, it needs a pre-hydrolysis process. Acid hydrolysis is a rapid hydrolysis method used for biological samples, which have been effectively applied for α-cellulose, benzo[a]pyrene tetrol, and DNA-adduct pretreatments in past studies.…”

Section: Introductionmentioning

confidence: 99%

Determination of 1-Hydroxypyrene in Human Urine by Acid Hydrolysis Coupled to Solid-Phase Microextraction and Semi-microcolumn Liquid Chromatography

Chen

2007

ANAL. SCI.

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Fast screening method for the profile analysis of polycyclic aromatic hydrocarbon metabolites in urine using derivatisation–solid-phase microextraction

Cited by 74 publications

References 17 publications

Direct measurement of the glucuronide conjugate of 1-hydroxypyrene in human urine by using liquid chromatography with tandem mass spectrometry

Direct measurement of the glucuronide conjugate of 1-hydroxypyrene in human urine by using liquid chromatography with tandem mass spectrometry

Modern Methods of Sample Preparation for GC Analysis

Determination of 1-Hydroxypyrene in Human Urine by Acid Hydrolysis Coupled to Solid-Phase Microextraction and Semi-microcolumn Liquid Chromatography

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