Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation

Gallego‐Selles, Angel; Galván-Alvarez, Victor; Martinez‐Canton, Miriam; Garcia-Gonzalez, Eduardo; Morales-Álamo, David; Santana, Alfredo; González-Henríquez, Juan José; Dorado, Cecilia; Calbet, J. A. L.; Martin‐Rincon, Marcos

doi:10.1016/j.redox.2022.102398

Cited by 13 publications

(10 citation statements)

References 85 publications

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“…The factors that affect the percentage of protein utilization during exercise include training status, exercise intensity, and the availability of other substrates (e.g., carbohydrates). From a molecular basis, it was reported that endurance exercise reduces the activation of nuclear factor-κ, leading to mitochondrial adaptations that prevent protein degradation [ 42 ]. The substrate utilization in this study depends on the respiratory quotation (RQ) that the IC calculates.…”

Section: Discussionmentioning

confidence: 99%

Resting Metabolic Rate and Substrate Utilization during Energy and Protein Availability in Male and Female Athletes

Abulmeaty,

Almajwal,

Elsayed

et al. 2024

Metabolites

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Active athletes frequently develop low energy (LEA) and protein availabilities (LPA) with consequent changes in the vital metabolic processes, especially resting metabolic rate (RMR) and substrate utilization. This study investigated the association of energy and protein intakes with RMR and substrate utilization in male and female athletes and those with LEA and LPA. Sixty athletes (35% female, 26.83 ± 7.12 y) were enrolled in this study. Anthropometric measurements and body composition analysis were reported to estimate fat-free mass (eFFM). Dietary intakes were recorded by two-day multiple-pass 24 h recall records and three-day food records and then analyzed by food processor software to calculate protein intake (PI) and energy intake (EI). Indirect calorimetry was used to measure RMR and percentages of substrate utilization. Activity–energy expenditure (AEE) was assessed by using an Actighrphy sensor for three days. Energy availability was calculated using the following formula (EA = EI − AEE/eFFM). The correlation of EI and PI with RMR and substrate utilization was tested with Pearson correlation. In the LEA group, both EI and PI correlated positively with RMR (r = 0.308, 0.355, respectively, p < 0.05). In addition, EI showed a positive correlation with the percentage of fat utilization. In the male and sufficient-PA groups, PI correlated positively with the RMR and negatively with the percentage of protein utilization. In conclusion, the percentage of LEA is markedly prevalent in our sample, with a higher prevalence among males. Athletes with LEA had lower fat utilization and lower RMR, while those with sufficient PA showed lower protein utilization with excessive PI. These findings may explain the metabolic responses in the cases of LEA and LPA.

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Section: Discussionmentioning

confidence: 99%

Resting Metabolic Rate and Substrate Utilization during Energy and Protein Availability in Male and Female Athletes

Abulmeaty,

Almajwal,

Elsayed

et al. 2024

Metabolites

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“…While the precise contribution of NF-κB signaling to the baseline gene expression pattern remains elusive, in the exercising muscle, we clearly could depict NF-κB together with the TF HIF1α as being among the major drivers of the acute exercise-provoked transcriptional stress response, both in SED as well as in ET individuals, as illustrated by a significant enrichment of related pathways in the exercise-provoked gene signatures. Both TFs have previously been shown to become activated in skeletal muscle during acute exercise, accumulate in the nucleus, and associate with chromatin at their target sites [ 51 , 74 , 75 ]. Moreover, there is increasing evidence that these two signaling pathways are intimately linked and coordinately act together in shaping exercise-provoked myofibrillar stress signaling to cope with acute shortages in energy and oxygen supplies, accumulation of reduced oxygen intermediates, perturbations in intracellular calcium levels, enforced mechanical strain at the contractile apparatus, and increased release of stress hormones [ 75 , 76 , 77 ].…”

Section: Discussionmentioning

confidence: 99%

The Acute, Short-, and Long-Term Effects of Endurance Exercise on Skeletal Muscle Transcriptome Profiles

Beiter,

Zügel,

Hudemann

et al. 2024

IJMS

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A better understanding of the cellular and molecular mechanisms that are involved in skeletal muscle adaptation to exercise is fundamentally important to take full advantage of the enormous benefits that exercise training offers in disease prevention and therapy. The aim of this study was to elucidate the transcriptional signatures that distinguish the endurance-trained and untrained muscles in young adult males (24 ± 3.5 years). We characterized baseline differences as well as acute exercise-induced transcriptome responses in vastus lateralis biopsy specimens of endurance-trained athletes (ET; n = 8; VO2max, 67.2 ± 8.9 mL/min/kg) and sedentary healthy volunteers (SED; n = 8; VO2max, 40.3 ± 7.6 mL/min/kg) using microarray technology. A second cohort of SED volunteers (SED-T; n = 10) followed an 8-week endurance training program to assess expression changes of selected marker genes in the course of skeletal muscle adaptation. We deciphered differential baseline signatures that reflected major differences in the oxidative and metabolic capacity of the endurance-trained and untrained muscles. SED-T individuals in the training group displayed an up-regulation of nodal regulators of oxidative adaptation after 3 weeks of training and a significant shift toward the ET signature after 8 weeks. Transcriptome changes provoked by 1 h of intense cycling exercise only poorly overlapped with the genes that constituted the differential baseline signature of ETs and SEDs. Overall, acute exercise-induced transcriptional responses were connected to pathways of contractile, oxidative, and inflammatory stress and revealed a complex and highly regulated framework of interwoven signaling cascades to cope with exercise-provoked homeostatic challenges. While temporal transcriptional programs that were activated in SEDs and ETs were quite similar, the quantitative divergence in the acute response transcriptomes implicated divergent kinetics of gene induction and repression following an acute bout of exercise. Together, our results provide an extensive examination of the transcriptional framework that underlies skeletal muscle plasticity.

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“…Цьому також сприяє здатність піролідиндиотіокарбамату амонію та диметилфумарату зменшувати метаболічні розлади, спричинені гіпомелатонінемією і ВЛД, зокрема інсулінорезистентність та дисліпопротеїнемію [13,14], які, як відомо, також сприяють виробленню АФК і АФА та прозапальних цитокінів [28]. Надмірна активація NF-κB виявляється особливо небезпечною для функціонування скелетних м'язів, наслідком чого є порушення їх скоротливості та атрофія [29], розвиток інсулінорезистентності [30].…”

Section: результати та їх обговоренняunclassified

Effect of Nf-Κb and Nrf2 Transcription Factor Modulators on Indicators of Oxidative– Nitrosative Stress in Skeletal Muscles of Rats Under Chronic Hypomelatoninemia and Carbohydrate-Lipid Diet

Frenkel¹,

Черно²,

Костенко³

2023

Fiziol Zh

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This article describes the effect of NF-κB inhibitor ammonium pyrrolidine dithiocarbamate and Nrf2 inducer dimethylfumarate on indicators of oxidative-nitrosative stress in skeletal muscles of rats with chronic hypomelatoninemia, induced by roundthe-clock illumination with an intensity of 1500 lux for 30 days, against the background of a carbohydrate-lipid diet (20% fructose solution and appropriate food) for 60 days. The study demonstrated that the administration of ammonium pyrrolidine dithiocarbamate and dimethylfumarate under the experimental conditions impedes the development of hypomelatoninemia, this was accompanied by a two-fold increase in the blood serum melatonin concentration compared to the control group. Moreover, the administration of ammonium pyrrolidine dithiocarbamate and dimethylfumarate significantly reduces the production of superoxide anion radical by electron transport chains of mitochondria (by 47.9 and 51.3%) and sarcoplasmic reticulum (by 48.6 and 52.0%) in the homogenate of femoral muscles, the total activity of NO synthase (by 37.2 and 36.2%) and its inducible isoform (by 41.1 and 40.0%), the concentration of peroxynitrites of alkaline and alkaline earth metals (by 37.2 and 41.0 %), while the activity of constitutive NO-synthases (by 2.9 and 2.8 times) and their conjugation index (by 5.2 and 5.4 times) increases compared to the respective control values. We suggest that modulators of redox-sensitive transcription factors NF-κB and Nrf2 under conditions of chronic hypomelatoninemia and the exposure to the carbohydrate-lipid diet are effective means to restrain the development of oxidative-nitrosative stress in skeletal muscles.

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Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation

Cited by 13 publications

References 85 publications

Resting Metabolic Rate and Substrate Utilization during Energy and Protein Availability in Male and Female Athletes

Resting Metabolic Rate and Substrate Utilization during Energy and Protein Availability in Male and Female Athletes

The Acute, Short-, and Long-Term Effects of Endurance Exercise on Skeletal Muscle Transcriptome Profiles

Effect of Nf-Κb and Nrf2 Transcription Factor Modulators on Indicators of Oxidative– Nitrosative Stress in Skeletal Muscles of Rats Under Chronic Hypomelatoninemia and Carbohydrate-Lipid Diet

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