Fast prototyping using a dry film photoresist: microfabrication of soft-lithography masters for microfluidic structures

Stephan, Khaled; Pittet, Patrick; Renaud, Louis; Kleimann, P.; Morin, Pierre; Ouaini, Naïm; Ferrigno, Rosaria

doi:10.1088/0960-1317/17/10/n01

Cited by 87 publications

(62 citation statements)

References 18 publications

(21 reference statements)

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“…The microfluidic devices are prepared using dry-film soft lithography techniques (41). The channels are etched in PDMS and bonded on a PDMS-covered glass slide.…”

Section: Methodsmentioning

confidence: 99%

Airway reopening through catastrophic events in a hierarchical network

Baudoin

Song

Manneville

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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When you reach with your straw for the final drops of a milkshake, the liquid forms a train of plugs that flow slowly initially because of the high viscosity. They then suddenly rupture and are replaced with a rapid airflow with the characteristic slurping sound. Trains of liquid plugs also are observed in complex geometries, such as porous media during petroleum extraction, in microfluidic two-phase flows, or in flows in the pulmonary airway tree under pathological conditions. The dynamics of rupture events in these geometries play the dominant role in the spatial distribution of the flow and in determining how much of the medium remains occluded. Here we show that the flow of a train of plugs in a straight channel is always unstable to breaking through a cascade of ruptures. Collective effects considerably modify the rupture dynamics of plug trains: Interactions among nearest neighbors take place through the wetting films and slow down the cascade, whereas global interactions, through the total resistance to flow of the train, accelerate the dynamics after each plug rupture. In a branching tree of microchannels, similar cascades occur along paths that connect the input to a particular output. This divides the initial tree into several independent subnetworks, which then evolve independently of one another. The spatiotemporal distribution of the cascades is random, owing to strong sensitivity to the plug divisions at the bifurcations. microfluidics | respiratory flow T he motion of liquid plugs through a connected network of channels may involve many degrees of freedom evolving via a similarly large number of interactions: each immiscible interface introduces a degree of freedom into the problem owing to its ability to deform and to move, whereas each connecting branch between different areas introduces an interaction path that allows the flow in one region to influence the behavior in other areas of the network. The resulting flow pattern determines, among other things, how water or oil is extracted from porous media (1-3), the imbibition of paper (4, 5), and the stability of flow in a microfluidic device (6, 7).Liquid-gas two-phase flows also occur in the pulmonary airway tree, which is constantly coated with a thin liquid film. When the thickness of this film increases beyond some limit, plugs of liquid may form (8, 9) and therefore occlude the flow of air to the distal branches. Evidence of such behavior has been observed in pathologies ranging from asthma (10, 11) to cystic fibrosis (12). Furthermore, liquid plugs may be used as means to deliver medical treatment into the lung, e.g., in surfactant replacement therapy (13), and these plugs were observed to go through complex divisions, breaking and reforming before reaching their intended target (14).The structure of the lung as a branching binary tree has motivated many studies on the motion of gas-liquid flows into bifurcating channels (15)(16)(17)(18)(19)(20), with numerical work also taking into account the elasticity of the pulmonary walls, (e.g refs. ...

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“…The microfluidic devices are prepared using dry-film soft lithography techniques (41). The channels are etched in PDMS and bonded on a PDMS-covered glass slide.…”

Section: Methodsmentioning

confidence: 99%

Airway reopening through catastrophic events in a hierarchical network

Baudoin

Song

Manneville

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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“…First, a PDMS microchannel was fabricated by replica molding using a dry film photoresist. 23 Then, a thin PDMS layer was coated onto the magnetic film surface to facilitate microchannel bonding by air plasma and to provide a biocompatible surface for cell attraction. This layer also serves to protect the magnetic film from corrosion.…”

Section: Microfluidic Integrationmentioning

confidence: 99%

Microfluidic immunomagnetic cell separation using integrated permanent micromagnets

et al. 2013

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In this paper, we demonstrate the possibility to trap and sort labeled cells under flow conditions using a microfluidic device with an integrated flat micro-patterned hard magnetic film. The proposed technique is illustrated using a cell suspension containing a mixture of Jurkat cells and HEK (Human Embryonic Kidney) 293 cells. Prior to sorting experiments, the Jurkat cells were specifically labeled with immunomagnetic nanoparticles, while the HEK 293 cells were unlabeled. Droplet-based experiments demonstrated that the Jurkat cells were attracted to regions of maximum stray field flux density while the HEK 293 cells settled in random positions. When the mixture was passed through a polydimethylsiloxane (PDMS) microfluidic channel containing integrated micromagnets, the labeled Jurkat cells were selectively trapped under fluid flow, while the HEK cells were eluted towards the device outlet. Increasing the flow rate produced a second eluate much enriched in Jurkat cells, as revealed by flow cytometry. The separation efficiency of this biocompatible, compact micro-fluidic separation chamber was compared with that obtained using two commercial magnetic cell separation kits.

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“…We have found that we can produce features down to as small as 40 µm in width (see Figure ? ?) without a collimated light source - (Stephan et al, 2007) could get no smaller than 200 µm without a collimated light source. Recognising the potential for our DFR protocols to be useful for others we have included them in the Appendix.…”

Section: Fabricationmentioning

confidence: 99%

Organising Chemical Reaction Networks in Space and Time with Microfluidics

Jones

Lovell

Morgan

et al. 2011

International Journal of Nanotechnology and Molecular Computation

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Information processing is essential for any lifeform to maintain its organisation despite continuous entropic disturbance. Macromolecules provide the ubiquitous underlying substrate on which nature implements information processing and have also come into focus for technical applications. There are two distinct approaches to the use of molecules for computing. Molecules can be employed to mimic the logic switches of conventional computers or they can be used in a way that exploits the complex functionality offered by a molecular computing substrate. Prerequisite to the latter is a mapping of input-output transform provided by the substrate. In the present paper we review microfluidic technology as a versatile means to achieve this, show how we use it, and provide proven recipes for its application.

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Fast prototyping using a dry film photoresist: microfabrication of soft-lithography masters for microfluidic structures

Cited by 87 publications

References 18 publications

Airway reopening through catastrophic events in a hierarchical network

Airway reopening through catastrophic events in a hierarchical network

Microfluidic immunomagnetic cell separation using integrated permanent micromagnets

Organising Chemical Reaction Networks in Space and Time with Microfluidics

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