Fast profiling of chemical constituents in Yiqing Capsule by ultra‐performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry

Abstract

An ultra-performance liquid chromatography/electrospray ionization tandem mass spectrometry (UPLC-ESI-MS(n)) has been developed for structural characterization and identification of multi-constituents in Yiqing Capsule, a well-known combined herbal remedy prepared from the extract mixtures of Rhizoma Coptidis, Radix et Rhizoma Rhei and Radix Scutellariae. The UPLC analysis was performed on an Agilent ZorBax SB-C(18) column (4.6 mm×50 mm, 1.8 μm) and gradient elution of 0.1% formic acid solution and acetonitrile in 16 min. Based on their retention times and mass spectra in comparison with the data from standards or references, a total of 29 compounds including 3 phenolic acids and 4 anthraquinones from Radix et Rhizoma Rhei, 8 alkaloids from Rhizoma Coptidis and 14 flavonoids from Radix Scutellariae were unambiguously identified or tentatively characterized in the complex system. The MS data and fragmentation information of two isomers of feruloylquinic acid were first reported in Radix et Rhizoma Rhei and in Yiqing Capsules. This study is expected to be accepted as an effective and reliable pattern for comprehensive and systematic characterization of this commonly used Chinese herbal preparation.

Systematically Characterizing Chemical Profile and Potential Mechanisms of Qingre Lidan Decoction Acting on Cholelithiasis by Integrating UHPLC-QTOF-MS and Network Target Analysis

“…By matching the constructed compound library, they were deduced to crocin-1 and crocin-4, a pair of cis-trans isomer originated from Gardeniae Fructus. In addition, as the polarity of cis-diterpenes was larger than that of trans-diterpenes, peaks 44 and 56 were identified a s crocin-1 and crocin-4, respectively [22, 24].…”

“…The fragment ion of m/z 181, 251, and 223 was corresponded to the losses of CO 2 , H 2 O, and CO, respectively (Figure 3(h)). Similarly, aloe-emodin, rhein, chrysophanol, and physcion were elucidated [24].…”

Determination of the variations in levels of phenolic compounds in soybean (Glycine max Merr.) sprouts infected by anthracnose (Colletotrichum gloeosporioides )

“…The structures and HPLC‐MS/MS data of the nine components are shown in Table . Tryptophan ( 1 ) and phenolic compounds ( 2–9 ) were previously reported from soybean germplasms …”

Simultaneous qualitative and quantitative analysis of 11 active compounds in rhubarb using two reference substances by UHPLC

“…However, the free anthraquinone compounds have different pharmacological effects compared with combined anthraquinone compounds. The previous study focused on the content of free anthraquinones and fingerprint analysis of rhubarb . In recent years, the main active ingredients, such as combined anthraquinone glycosides, have attracted increasing attention .…”