Fast preparation of nano-hydroxyapatite/superhydrophilic reduced graphene oxide composites for bioactive applications

Zanin, Hudson; Saito, Eduardo; Marciano, Fernanda Roberta; Ceragioli, Helder José; Granato, Alessandro E.C.; Porcionatto, Marimélia; Lobo, Anderson Oliveira

doi:10.1039/c3tb20550a

Cited by 64 publications

(57 citation statements)

References 51 publications

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“…Moreover, a hydrogen bond interaction between the oxygen-containing functional groups of rGO nanosheets (e.g. hydroxyl, epoxy, carboxyl, and carbonyl groups) and hydroxyl groups of HAp microparticles can also contribute to the strong adhesion between rGO nanosheets and HAp microparticles [36]. Therefore, it is indicated that the rGO/HAp composites can maintain their structure in the culture media and are stable under cell culture condition.…”

Section: Characterizations Of Rgo/hap Compositesmentioning

confidence: 97%

“…There was no significant difference in the Raman spectra before and after incubation in the media. Previous studies have already revealed that HAp composites with rGO can be formed by electrostatic interactions between rGO nanosheets and HAp microparticles [20,36,44]. Moreover, a hydrogen bond interaction between the oxygen-containing functional groups of rGO nanosheets (e.g.…”

Section: Characterizations Of Rgo/hap Compositesmentioning

confidence: 98%

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Synergistic effects of reduced graphene oxide and hydroxyapatite on osteogenic differentiation of MC3T3-E1 preosteoblasts

Shin

Lee

Jin

et al. 2015

Carbon

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Section: Characterizations Of Rgo/hap Compositesmentioning

confidence: 97%

Section: Characterizations Of Rgo/hap Compositesmentioning

confidence: 98%

Synergistic effects of reduced graphene oxide and hydroxyapatite on osteogenic differentiation of MC3T3-E1 preosteoblasts

Shin

Lee

Jin

et al. 2015

Carbon

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“…Therefore, the DCP-rGO composites could be successfully formed because of the unique structural properties of rGO sheets. In addition, the interfacial and hydrogen bond interactions between the oxygen-containing surface groups of rGO and the hydroxyl groups of DCP microparticles could also participate in the good adhesion between DCP microparticles and rGO [50,57,58]. Figure 3b showed the Raman spectra of DCP microparticles and DCP-rGO composites.…”

Section: Methodsmentioning

confidence: 99%

Dicalcium Phosphate Coated with Graphene Synergistically Increases Osteogenic Differentiation In Vitro

et al. 2017

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Abstract:In recent years, graphene and its derivatives have attracted much interest in various fields, including biomedical applications. In particular, increasing attention has been paid to the effects of reduced graphene oxide (rGO) on cellular behaviors. On the other hand, dicalcium phosphate (DCP) has been widely used in dental and pharmaceutical fields. In this study, DCP composites coated with rGO (DCP-rGO composites) were prepared at various concentration ratios (DCP to rGO concentration ratios of 5:2.5, 5:5, and 5:10 µg/mL, respectively), and their physicochemical properties were characterized. In addition, the effects of DCP-rGO hybrid composites on MC3T3-E1 preosteoblasts were investigated. It was found that the DCP-rGO composites had an irregular granule-like structure with a diameter in the range order of the micrometer, and were found to be partially covered and interconnected with a network of rGO. The zeta potential analysis showed that although both DCP microparticles and rGO sheets had negative surface charge, the DCP-rGO composites could be successfully formed by the unique structural properties of rGO. In addition, it was demonstrated that the DCP-rGO composites significantly increased alkaline phosphatase activity and extracellular calcium deposition, indicating that the DCP-rGO hybrid composites can accelerate the osteogenic differentiation by the synergistic effects of rGO and DCP. Therefore, in conclusion, it is suggested that the DCP-rGO hybrid composites can be potent factors in accelerating the bone tissue regeneration.

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“…The G band is assigned to one of the two E 2 g modes corresponding to stretching vibrations in the basal plane (sp 2 domains) of single-crystal graphene. 31 The high-intensity G′ band reveals that these materials present high structural quality. 32 In the MWCNT-GO first-order Raman …”

Section: Nanocomposites Characterizationmentioning

confidence: 99%

“…Probably, the shoulder has its origin in the double resonance process because its Raman shift (~1,200 cm ) is a point on the graphene phonon dispersion curves. 31 The origin of the 1,480 cm -1 (*) band is probably correlated with the polar group grafting onto the MWCNT-GO surfaces. 33 It is well known that MWCNTs are graphene sheets rolled into cylinders.…”

mentioning

confidence: 99%

Graphene oxide/multi-walled carbon nanotubes as nanofeatured scaffolds for the assisted deposition of nanohydroxyapatite: characterization and biological evaluation

Rodrigues¹,

Leite²,

Cavalcanti³

et al. 2016

IJN

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Nanohydroxyapatite (nHAp) is an emergent bioceramic that shows similar chemical and crystallographic properties as the mineral phase present in bone. However, nHAp presents low fracture toughness and tensile strength, limiting its application in bone tissue engineering. Conversely, multi-walled carbon nanotubes (MWCNTs) have been widely used for composite applications due to their excellent mechanical and physicochemical properties, although their hydrophobicity usually impairs some applications. To improve MWCNT wettability, oxygen plasma etching has been applied to promote MWCNT exfoliation and oxidation and to produce graphene oxide (GO) at the end of the tips. Here, we prepared a series of nHAp/MWCNT-GO nanocomposites aimed at producing materials that combine similar bone characteristics (nHAp) with high mechanical strength (MWCNT-GO). After MWCNT production and functionalization to produce MWCNT-GO, ultrasonic irradiation was employed to precipitate nHAp onto the MWCNT-GO scaffolds (at 1–3 wt%). We employed various techniques to characterize the nanocomposites, including transmission electron microscopy (TEM), Raman spectroscopy, thermogravimetry, and gas adsorption (the Brunauer–Emmett–Teller method). We used simulated body fluid to evaluate their bioactivity and human osteoblasts (bone-forming cells) to evaluate cytocompatibility. We also investigated their bactericidal effect against Staphylococcus aureus and Escherichia coli . TEM analysis revealed homogeneous distributions of nHAp crystal grains along the MWCNT-GO surfaces. All nanocomposites were proved to be bioactive, since carbonated nHAp was found after 21 days in simulated body fluid. All nanocomposites showed potential for biomedical applications with no cytotoxicity toward osteoblasts and impressively demonstrated a bactericidal effect without the use of antibiotics. All of the aforementioned properties make these materials very attractive for bone tissue engineering applications, either as a matrix or as a reinforcement material for numerous polymeric nanocomposites.

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Fast preparation of nano-hydroxyapatite/superhydrophilic reduced graphene oxide composites for bioactive applications

Cited by 64 publications

References 51 publications

Synergistic effects of reduced graphene oxide and hydroxyapatite on osteogenic differentiation of MC3T3-E1 preosteoblasts

Synergistic effects of reduced graphene oxide and hydroxyapatite on osteogenic differentiation of MC3T3-E1 preosteoblasts

Dicalcium Phosphate Coated with Graphene Synergistically Increases Osteogenic Differentiation In Vitro

Graphene oxide/multi-walled carbon nanotubes as nanofeatured scaffolds for the assisted deposition of nanohydroxyapatite: characterization and biological evaluation

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