2009
DOI: 10.1021/ac901054w
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Fast Photochemical Oxidation of Protein Footprints Faster than Protein Unfolding

Abstract: Fast photochemical oxidation of proteins (FPOP) is a chemical footprinting method whereby exposed amino-acid residues are covalently labeled by oxidation with hydroxyl radicals produced by the photolysis of hydrogen peroxide. Modified residues can be detected by standard trypsin proteolysis followed by LC/MS/MS, providing information about solvent accessibility at the peptide and even the amino-acid level. Like other chemical footprinting techniques, FPOP must ensure only the native conformation is labeled. Al… Show more

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Cited by 195 publications
(279 citation statements)
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“…The ⋅OH concentration profile calculated under pseudo-first order conditions for [Gln] = 20 mM drops close to zero within 1 μs (blue line, Figure 1b) [30]. This plot represents the foundation of the claim that FPOP is a microsecond covalent labeling technique [30][31][32].…”
Section: Introductionmentioning
confidence: 64%
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“…The ⋅OH concentration profile calculated under pseudo-first order conditions for [Gln] = 20 mM drops close to zero within 1 μs (blue line, Figure 1b) [30]. This plot represents the foundation of the claim that FPOP is a microsecond covalent labeling technique [30][31][32].…”
Section: Introductionmentioning
confidence: 64%
“…The acronym FPOP (fast photochemical oxidation of proteins) has been coined for this technique [31]. It was claimed that FPOP goes to completion within~1 μs.…”
Section: Introductionmentioning
confidence: 99%
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