Fast Neutron Mutagenesis of Soybean (&lt;I&gt;Glycine soja&lt;/I&gt; L.) Produces a Supernodulating Mutant Containing a Large Deletion in Linkage Group H

Men, A.; Laniya, T. S.; Searle, Iain; Iturbe‐Ormaetxe, Iñaki; Gresshoff, Irma; Jiang, Qunyi; Carroll, Bernard J.; Gresshoff, Peter M.

doi:10.1166/gl.2002.017

Cited by 53 publications

(27 citation statements)

References 32 publications

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“…It is also possible that a single deviant data point on the CGH array could represent a true deletion; however, such a region would not be identified using our stringent filtering criteria, and the exact break points for fragmented deletion borders may not be resolved using CGH alone. The size of FN-induced deletions defined in this study range from less than 1 kb to almost 3 Mb and expands the range of FNinduced deletions reported in plants (Li et al, 2001;Men et al, 2002). However, an underestimation of structural variation events, particularly of smaller deletions and duplications, is likely to occur.…”

Section: Genomic Mutation Detection Methodssupporting

confidence: 49%

“…The majority of mutations that result from FN bombardment are DNA deletions that range in size from a few base pairs to several megabases (Li et al, 2001;Men et al, 2002). Precedence exists in many species, including Arabidopsis (Arabidopsis thaliana; Alonso et al, 2003), Medicago truncatula (Oldroyd and Long, 2003), Glycine soja (Searle et al, 2003), barley (Hordeum vulgare; Zhang et al, 2006b), and Lotus japonicus (Hoffmann et al, 2007), for the use of FN mutagenesis in forward genetic screens.…”

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confidence: 99%

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Phenotypic and Genomic Analyses of a Fast Neutron Mutant Population Resource in Soybean

et al. 2011

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Mutagenized populations have become indispensable resources for introducing variation and studying gene function in plant genomics research. In this study, fast neutron (FN) radiation was used to induce deletion mutations in the soybean (Glycine max) genome. Approximately 120,000 soybean seeds were exposed to FN radiation doses of up to 32 Gray units to develop over 23,000 independent M2 lines. Here, we demonstrate the utility of this population for phenotypic screening and associated genomic characterization of striking and agronomically important traits. Plant variation was cataloged for seed composition, maturity, morphology, pigmentation, and nodulation traits. Mutants that showed significant increases or decreases in seed protein and oil content across multiple generations and environments were identified. The application of comparative genomic hybridization (CGH) to lesion-induced mutants for deletion mapping was validated on a midoleate x-ray mutant, M23, with a known FAD2-1A (for fatty acid desaturase) gene deletion. Using CGH, a subset of mutants was characterized, revealing deletion regions and candidate genes associated with phenotypes of interest. Exome resequencing and sequencing of PCR products confirmed FN-induced deletions detected by CGH. Beyond characterization of soybean FN mutants, this study demonstrates the utility of CGH, exome sequence capture, and next-generation sequencing approaches for analyses of mutant plant genomes. We present this FN mutant soybean population as a valuable public resource for future genetic screens and functional genomics research.

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Section: Genomic Mutation Detection Methodssupporting

confidence: 49%

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confidence: 99%

Phenotypic and Genomic Analyses of a Fast Neutron Mutant Population Resource in Soybean

et al. 2011

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“…Eleven out of those 13 double and triple mutants were generated using neutron mutagenesis (von Wettstein-Knowles and Søgaard, 1980). This mutagenesis procedure is known to cause DNA deletions, ranging in size from kilobases to megabases (Li et al, 2001;Men et al, 2002); therefore, revertants are highly unlikely to occur. Indeed, here, we show a correlation between the barley mutant groups (cer-c, q, and u) and mutations in DMP, DMH, and DMC that clearly correspond to a metabolic gene cluster and not to a multifunctional gene.…”

Section: Multiple Genes Rather Than a Single One Encoding A Multifuncmentioning

confidence: 99%

A Metabolic Gene Cluster in the Wheat W1 and the Barley Cer-cqu Loci Determines β-Diketone Biosynthesis and Glaucousness

et al. 2016

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The glaucous appearance of wheat (Triticum aestivum) and barley (Hordeum vulgare) plants, that is the light bluish-gray look of flag leaf, stem, and spike surfaces, results from deposition of cuticular b-diketone wax on their surfaces; this phenotype is associated with high yield, especially under drought conditions. Despite extensive genetic and biochemical characterization, the molecular genetic basis underlying the biosynthesis of b-diketones remains unclear. Here, we discovered that the wheat W1 locus contains a metabolic gene cluster mediating b-diketone biosynthesis. The cluster comprises genes encoding proteins of several families including type-III polyketide synthases, hydrolases, and cytochrome P450s related to known fatty acid hydroxylases. The cluster region was identified in both genetic and physical maps of glaucous and glossy tetraploid wheat, demonstrating entirely different haplotypes in these accessions. Complementary evidence obtained through gene silencing in planta and heterologous expression in bacteria supports a model for a b-diketone biosynthesis pathway involving members of these three protein families. Mutations in homologous genes were identified in the barley eceriferum mutants defective in b-diketone biosynthesis, demonstrating a gene cluster also in the b-diketone biosynthesis Cer-cqu locus in barley. Hence, our findings open new opportunities to breed major cereal crops for surface features that impact yield and stress response.

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“…Developments are under way to expedite gene cloning using the NimbleGen tiling array (R. Duvvuru Muni and R. Chen, personal communication). FNB has also been used in soybean (Men et al, 2002) and is being developed as a resource for this legume (http://rhdev. webapps.uga.edu/staging/soy_nsf/fastneutron/index.…”

Section: Deletion Mutagenesismentioning

confidence: 99%

Mutagenesis and Beyond! Tools for Understanding Legume Biology

et al. 2009

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Fast Neutron Mutagenesis of Soybean (<I>Glycine soja</I> L.) Produces a Supernodulating Mutant Containing a Large Deletion in Linkage Group H

Cited by 53 publications

References 32 publications

Phenotypic and Genomic Analyses of a Fast Neutron Mutant Population Resource in Soybean

Phenotypic and Genomic Analyses of a Fast Neutron Mutant Population Resource in Soybean

A Metabolic Gene Cluster in the Wheat W1 and the Barley Cer-cqu Loci Determines β-Diketone Biosynthesis and Glaucousness

Mutagenesis and Beyond! Tools for Understanding Legume Biology

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