Fast Morphological Gallbladder Changes Triggered by a Hypercholesterolemic Diet

López‐Reyes, Alberto; Clavijo-Cornejo, Denise; Fernández‐Torres, Javier; Medina-Luna, Daniel; Estrada-Villaseñor, Erendida G; Gómez-Quiroz, Luís Enrique; Gutiérrez, Marwin; Granados, Julio; Vargas‐Alarcón, Gilberto; Pineda, Carlos; García, Hiram; Morales-Garza, Luis Alonso; Gutiérrez‐Ruiz, María Concepción; Martínez-Flores, Karina

doi:10.5604/01.3001.0012.3160

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…Clinical and experimental studies have found significant relationship between intestinal cholesterol absorption and cholesterol gallstone formation [ 6 , 24 ]. Targeted inhibition of intestinal cholesterol absorption, e.g.…”

Section: Discussionmentioning

confidence: 99%

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Dietary diosgenin transcriptionally down-regulated intestinal NPC1L1 expression to prevent cholesterol gallstone formation in mice

Shen,

Shao,

Wang

et al. 2023

J Biomed Sci

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Background Cholesterol gallstone disease is a common disease. Reducing cholesterol burden is important to prevent/treat gallstone. In this study, we investigated the application of diosgenin (DG) to prevent the formation of gallstone in mice. Methods Adult male C57BL/6J mice were fed with the lithogenic diet (LD) only or LD supplemented with DG or ezetimibe for 8 weeks. Incidences of gallstone formation were documented. Intestine and liver tissues were collected to measure the lipid contents and expression of genes in cholesterol metabolism. Caco2 cells were treated with DG to monitor the regulation on cholesterol absorption and the transcriptional regulation of Npc1l1 gene. Changes of gut microbiota by DG was analyzed. Intraperitoneal injection of LPS on mice was performed to verify its effects on STAT3 activation and Npc1l1 expression in the small intestine. Results LD led to 100% formation of gallstones in mice. In comparison, dietary DG or ezetimibe supplementary completely prevents gallstones formation. DG inhibited intestinal cholesterol absorption in mice as well as in Caco2 cells by down-regulation of Npc1l1 expression. DG could directly inhibit phosphorylation of STAT3 and its transcriptional regulation of Npc1l1 expression. Furthermore, DG could modulate gut microbiota profiles and LPS mediated STAT3 activation and Npc1l1 expression. Conclusion Our results demonstrated that dietary DG could inhibit intestinal cholesterol absorption through decreasing NPC1L1 expression to prevent cholesterol gallstone formation.

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Section: Discussionmentioning

confidence: 99%

“…Supersaturation of biliary cholesterol is known to be a prerequisite for gallstone formation [ 5 ]. Gut-derived excess dietary cholesterol is an important source of biliary cholesterol [ 6 ]. Therefore, inhibition of intestinal cholesterol absorption is considered to be a promising therapeutic measure to prevent gallstone formation.…”

Section: Introductionmentioning

confidence: 99%

Dietary diosgenin transcriptionally down-regulated intestinal NPC1L1 expression to prevent cholesterol gallstone formation in mice

Shen,

Shao,

Wang

et al. 2023

J Biomed Sci

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show abstract

“…Nuestro grupo y otros grupos de investigación hemos reportado que es el tipo de lípido más que la cantidad de este, es lo que determina el grado de daño celular (Dominguez-Perez et al, 2019;Enriquez-Cortina et al, 2017;Mari et al, 2006), en particular, el incremento en el colesterol hepático gobierna la gravedad de las enfermedades hepáticas (Lopez-Islas et al, 2016;Lopez-Reyes et al, 2018;Nuno-Lambarri et al, 2016). En nuestro modelo in vitro, mostramos que el contenido de colesterol aumenta significativamente en hepatocitos.…”

Section: Discussionunclassified

La mezcla de ácidos grasos de tipo mediterráneo induce protección celular en hepatocitos con sobrecarga de lípidos en un modelo Murino de esteatosis inducido con una dieta tipo Western

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Mediterranean-like mix of fatty acids induces cellular protection on lipid-overloaded hepatocytes from western diet fed mice

Castellanos-Tapia

Tejero

Salas-Silva

et al. 2020

Annals of Hepatology

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Fast Morphological Gallbladder Changes Triggered by a Hypercholesterolemic Diet

Abstract: Cholesterol overloads not only trigger hepatic damage, but also affect the gallbladder significantly.

Cited by 4 publications

References 23 publications

Dietary diosgenin transcriptionally down-regulated intestinal NPC1L1 expression to prevent cholesterol gallstone formation in mice

Dietary diosgenin transcriptionally down-regulated intestinal NPC1L1 expression to prevent cholesterol gallstone formation in mice

La mezcla de ácidos grasos de tipo mediterráneo induce protección celular en hepatocitos con sobrecarga de lípidos en un modelo Murino de esteatosis inducido con una dieta tipo Western

Mediterranean-like mix of fatty acids induces cellular protection on lipid-overloaded hepatocytes from western diet fed mice

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