Fast monolith-based chromatographic method for determination of methotrexate in drug delivery studies

Barbosa, Ana Isabel; Fernandes, Sara R.; Machado, Sandia; Sousa, Patrícia; Ong, Yong Sze; Silva, Eduarda M. P.; Barreiros, Luísa; Lima, Sofia A. Costa; Segundo, Marcela A.

doi:10.1016/j.microc.2019.04.075

Cited by 5 publications

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The HPLC system and the chromatographic conditions used in this work were described in detail elsewhere [49]. The analysis of MTX standard solutions and diluted nanoparticle batches (analyzed without any pretreatment or after ultrafiltration process) were performed in a Jasco HPLC system (Easton, USA) composed by a PU-2089 pump, an AS-2057 autosampler, a LC-Net II/ADC controller, and a Jasco MD-2015 photo diode array detector.…”

Section: High-performance Liquid Chromatography Methodsmentioning

confidence: 99%

Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers

et al. 2020

Self Cite

View full text Add to dashboard Cite

The evaluation of encapsulation efficiency is a regulatory requirement for the characterization of drug delivery systems. However, the difficulties in efficiently separating nanomedicines from the free drug may compromise the achievement of accurate determinations. Herein, ultrafiltration was exploited as a separative strategy towards the evaluation of methotrexate (MTX) encapsulation efficiency in nanostructured lipid carriers and polymeric nanoparticles. The effect of experimental conditions such as pH and the amount of surfactant present in the ultrafiltration media was addressed aiming at the selection of suitable conditions for the effective purification of nanocarriers. MTX-loaded nanoparticles were then submitted to ultrafiltration and the portions remaining in the upper compartment of the filtering device and in the ultrafiltrate were collected and analyzed by HPLC-UV using a reversed-phase (C18) monolithic column. A short centrifugation time (5 min) was suitable for establishing the amount of encapsulated MTX in nanostructured lipid carriers, based on the assumption that the free MTX concentration was the same in the upper compartment and in the ultrafiltrate. The defined conditions allowed the efficient separation of nanocarriers from the free drug, with recoveries of >85% even when nanoparticles were present in cell culture media and in pig skin surrogate from permeation assays.

show abstract

Section: High-performance Liquid Chromatography Methodsmentioning

confidence: 99%

Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Methotrexate (MTX) is derived from the folic acid antagonist and has been used widely in chemotherapy as a cure for various cancer diseases including leukemia, breast cancer, lung cancer, osteosarcoma, and lymphoma. MTX is also used as cure for the autoimmune diseases like rheumatoid arthritis, psoriasis and Crohn,s illness [1][2][3]. MTX is also effective for the head and neck cancer.…”

Section: Introductionmentioning

confidence: 99%

Polyaniline as a Sacrificing Template for the Synthesis of Ultra-small Co3O4 Nanoparticles for the Sensitive and Selective Detection of Methotrexate (MTX)

Qureshi

Tahira

Aljadoa

et al. 2021

Preprint

View full text Add to dashboard Cite

The successful monitoring of the anticancer drugs using nanostructured materials is very important but very challenging task. Beside this, uniform and ultra-small size of metal oxide nanoparticles is highly needed in order to enhance the catalytic activity which could result into the development of sensitive and selective electrochemical sensors for methotrexate (MTX). For this purpose, we have used a simple approach involving the polyaniline (PANI) as a sacrificing template for the growth of uniform and ultra-small Co3O4 nanoparticles by hydrothermal method. The structure, shape, composition and phase purity were studied by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) and Fourier transform Infrared (FTIR) techniques. The average size of Co3O4 nanoparticles was below 50 nm. The cubic crystallography is confirmed for the Co3O4 nanoparticles. The electrochemical properties of PANI assisted Co3O4 nanoparticles for MTX drug was evaluated by cyclic voltammetry (CV) and linear sweep voltammetry (LSV) in Britton–Robinson buffer (BRB) of pH 3.5. The PANI assisted Co3O4 nanoparticles were found highly sensitive for the MTX drug and exhibited a linear range from 5-75µM of MTX and limit of detection for the modified electrode was estimated 1.98µM. The proposed electrochemical sensor is low cost, simple, highly sensitive and selective towards MTX detection. The synthetic methodology using the conducting polymer as a sacrificing template for the growth of controlled and ultra-small Co3O4 nanoparticles can be utilized for the wide range of electrochemical applications.

show abstract

A novel three-dimensional network of CuCr2O4/CuO nanofibers for voltammetric determination of anticancer drug methotrexate

2020

View full text Add to dashboard Cite

Fast monolith-based chromatographic method for determination of methotrexate in drug delivery studies

Cited by 5 publications

References 35 publications

Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers

Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers

Polyaniline as a Sacrificing Template for the Synthesis of Ultra-small Co3O4 Nanoparticles for the Sensitive and Selective Detection of Methotrexate (MTX)

A novel three-dimensional network of CuCr2O4/CuO nanofibers for voltammetric determination of anticancer drug methotrexate

Contact Info

Product

Resources

About