Fast Microchip Electrophoresis Using Field Strength Gradients for Single Nucleotide Polymorphism Identification of Cattle Breeds

Oh, Doori; Cheong, I. C.; Lee, Hee Gu; Eo, Seong Kug; Kang, Seong Ho

doi:10.5012/bkcs.2010.31.7.1902

Cited by 5 publications

(1 citation statement)

References 17 publications

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“…In the CGE, when a uniform electric field ( E ) is applied to a DNA molecule, the electrophoretic velocity ( v ep ) can be represented as the product of the electrophoretic mobility (

μ_{ep}

) of a DNA molecule and the electric field ( E ) .…”

Section: Introductionmentioning

confidence: 99%

Voltage‐programming‐based capillary gel electrophoresis for the fast detection of angiotensin‐converting enzyme insertion/deletion polymorphism with high sensitivity

Woo

Kim

Kang

2016

J of Separation Science

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A voltage-programming-based capillary gel electrophoresis method with a laser-induced fluorescence detector was developed for the fast and highly sensitive detection of DNA molecules related to angiotensin-converting enzyme insertion/deletion polymorphism, which has been reported to influence predisposition to various diseases such as cardiovascular disease, high blood pressure, myocardial infarction, and Alzheimer's disease. Various voltage programs were investigated for fast detection of specific DNA molecules of angiotensin-converting enzyme insertion/deletion polymorphism as a function of migration time and separation efficiency to establish the effect of voltage strength to resolution. Finally, the amplified products of the angiotensin-converting enzyme insertion/deletion polymorphism (190 and 490 bp DNA) were analyzed in 3.2 min without losing resolution under optimum voltage programming conditions, which were at least 75 times faster than conventional slab gel electrophoresis. In addition, the capillary gel electrophoresis method also successfully applied to the analysis of real human blood samples, although no polymorphism genes were detected by slab gel electrophoresis. Consequently, the developed voltage-programming capillary gel electrophoresis method with laser-induced fluorescence detection is an effective, rapid analysis technique for highly sensitive detection of disease-related specific DNA molecules.

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“…In the CGE, when a uniform electric field ( E ) is applied to a DNA molecule, the electrophoretic velocity ( v ep ) can be represented as the product of the electrophoretic mobility (

μ_{ep}

) of a DNA molecule and the electric field ( E ) .…”

Section: Introductionmentioning

confidence: 99%

Voltage‐programming‐based capillary gel electrophoresis for the fast detection of angiotensin‐converting enzyme insertion/deletion polymorphism with high sensitivity

Woo

Kim

Kang

2016

J of Separation Science

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Fast and highly sensitive screening of PCR products of severe acute respiratory syndrome coronavirus 2 by voltage‐programmed capillary gel electrophoretic separation

Park

Kim

et al. 2023

Bulletin Korean Chem Soc

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Fast and highly sensitive detection has become critical to prevent the spread of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). However, fast analysis tends to reduce the sensitivity and accuracy of such method. This study is to investigate a fast and highly sensitive screening method for detecting polymerase chain reaction (PCR) products of SARS‐CoV‐2 through voltage‐programmed capillary gel electrophoretic separation. By separating and detecting the PCR products of RNA‐dependent RNA polymerase (RdRp) and spike (S) genes, we have diagnosed coronavirus disease 2019 (COVID‐19) cases within 4 min using such method with high reliability and accuracy, indicating at least a 7.5‐fold faster than previous COVID‐19 diagnostic methods. The detection limits of RdRp and S genes fragments were 1.34–3.46 pM. The developed method can be used to analyze PCR products with specific DNA sizes as well as detect SARS‐CoV‐2 mutations without losing resolution. Furthermore, it can also be used for the fast analysis of DNA molecules related with various infectious diseases.

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Food analysis on microchip electrophoresis: An updated review

2012

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From 2008 to date, basically, single‐cross microchip electrophoresis (ME) design has been used for food analysis with electrochemicaland laser‐induced fluorescencedetection being the most commonprinciples coupled. In the last 4 years, the main outlines were: (i) the exploration of new analytes such as heavy metals, nitrite, micotoxins, microorganisms, and allergens; (ii) the development of electrokinetic microfluidic (bio‐) sensors into microchip format for the detection of toxins; and interestingly (iii) although sample preparation is still performed off‐chip, an important increase in works dealing with complicated food samples has been clearly noticed. Although microchip technology based on electrokinetics is emerging from important fields such as authentication of foods, detection of frauds, toxics, and allergens; the marriage between micro‐ and nanotechnologies and total integration approaches has not reached the expected impact in the field but it is still a great promise for the development of ME of new generations for food analysis.

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Fast Microchip Electrophoresis Using Field Strength Gradients for Single Nucleotide Polymorphism Identification of Cattle Breeds

Cited by 5 publications

References 17 publications

Voltage‐programming‐based capillary gel electrophoresis for the fast detection of angiotensin‐converting enzyme insertion/deletion polymorphism with high sensitivity

Voltage‐programming‐based capillary gel electrophoresis for the fast detection of angiotensin‐converting enzyme insertion/deletion polymorphism with high sensitivity

Fast and highly sensitive screening of PCR products of severe acute respiratory syndrome coronavirus 2 by voltage‐programmed capillary gel electrophoretic separation

Food analysis on microchip electrophoresis: An updated review

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