Fast liquid chromatographic-tandem mass spectrometric (LC–MS–MS) determination of metformin in plasma samples

Heinig, Katja; Bucheli, Franz

doi:10.1016/j.jpba.2003.11.017

Cited by 71 publications

(43 citation statements)

References 10 publications

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“…There are numerous methods that can measure metformin within biological fluids. These include capillary electrophoresis [10,11] and high performance liquid chromatography (HPLC) [12][13][14]. The latter being the most widely applied technique and is routinely linked to tandem mass spectrometry (MS/MS).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Michel

Gaunt

Arnason

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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A simple, fast and sensitive quantification method for the drug metformin in dog serum was developed using flow injection analysis (FIA)-tandem mass spectrometry (MS/MS). The method was fully validated according to industry standards. It is the first time that FIA-MS/MS for metformin was developed surpassing all existing methods in terms of time of analysis. The quantification method was dependent on the formation of [M+H] + using electrospray ionization (ESI) and employing multiple reaction monitoring (MRM) using quadrupole-linear ion trap (4000 QTRAP ® ) instrument. A deuterated internal standard (IS) of metformin bearing six deuterium atoms was used to compensate for matrix effects and for variation in ion current within the ESI source. The ion transitions that were monitored were m/z 130.1 m/z 71.0 and m/z 130.1 m/z 60.1 for metformin and m/z 136.0 m/z 77.0 for the internal standard. A linear response (r = 0.9966) was established for a range of concentrations of 5-2340 ng/ml. The inter-and intra-day variations were within the acceptable criteria for all quality control samples. The method was successfully applied for measurement of serum metformin concentration in dogs after intravenous injection.

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Section: Introductionmentioning

confidence: 99%

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Michel

Gaunt

Arnason

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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“…Type 2 diabetes is progressive in nature and glycemic control become difficult in most patients, requiring either increased Metformin dosage or supplementary (add-on) therapy. 6 A survey of the literature revealed some HPLC and LC-MS/MS methods for analysis of MET alone [7][8][9][10][11][12][13] and in combination with other drugs. However, quite a few HPLC methods have been reported for simultaneous determination of MET with SIT [14][15][16][17][18][19][20][21][22] or VLD [23][24][25][26][27][28] or LIN 29,30 in formulations, but, no simple and rapid method is yet established for simultaneous determination of Metformin, Sitagliptin, Vildagliptin and Linagliptin using single analytical procedure.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Metformin and Three Gliptins in Pharmaceutical Formulations Using RP HPLC: Application to Stability Studies on Linagliptin Tablet Formulation

Attimarad¹,

Nagaraja²,

Aldhubaib³

et al. 2014

IJPER

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Objective: A simple, rapid and validated liquid chromatographic method has been developed for the simultaneous determination of three novel Gliptins namely Vildagliptin (VLD), Sitagliptin (SIT) and Linagliptin (LIN) in their binary mixture with Metformin (MET). Methodology: The separation was performed on fast monolithic column using isocratic, mobile phase consisting of mixture of sodium dihydrogen phosphate, Sodium dedosyl sulphate and acetonitrile. The flow rate was 2.5 mL/min and UV detection for MET, VLD and SIT was conducted at 208 nm, whereas, that of MET and LIN was at 228 nm. The incipiently developed method was subjected for validation according to ICH guidelines. Results: The calibration curves of analytes showed good correlation (r2>0.999) over a concentration range 10-100 μg/mL and 50-400 μg/mL for Metformin, 1-10 μg/mL for Vildagliptin & Sitagliptin and 0.25-2.0 μg/mL for Linigliptin. Conclusion: All three tablet formulations were assayed with accuracy and precision and without interference from excipients. The method is also stability indicating with respect to Linagliptin.

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“…A sur vey of the literature revealed some highperformance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry methods for estimation of MET alone [9][10][11][12][13][14][15] and in combination with other drugs. Few analytical methods are documented in the literature for determination of VLD in solid dosage forms 16,17 or in plasma.…”

Section: Introductionmentioning

confidence: 99%

Development of a rapid reversed phase-high performance liquid chromatography method for simultaneous determination of metformin and vildagliptin in formulation and human plasma

Attimarad¹,

Nagaraja²,

Aldhubaib³

et al. 2014

JYP

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Objective: The objective of the proposed method was to develop a simple, fast, sensitive, and validated high-performance liquid chromatography (HPLC) method for the simultaneous estimation of metformin and vildagliptin in tablets and human plasma. Materials and Methods: The chromatographic separation was accomplished with a fast monolithic column using a mixture of sodium dihydrogen phosphate and sodium dodecyl sulfate and acetonitrile at pH 4.5 as the mobile phase. In addition, newly developed method was validated as indicated by International Conference on Harmonization guidelines. Results: The method showed good correlation coeffi cients (r ≥ 0.997) in the range of 0.05-20 μg/mL and 0.1-40 μg/mL for metformin and vildagliptin, respectively. The accuracy and intraday and interday precision results were within the acceptable range for both analytes. The mean extraction recoveries of metformin and vildagliptin from human plasma were 97.51% and 97.18%, respectively. Conclusion: The simple, rapid, sensitive, robust and validated analytical method developed was used for simultaneous estimation of metformin and vildagliptin in formulation and in patient plasma.

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Fast liquid chromatographic-tandem mass spectrometric (LC–MS–MS) determination of metformin in plasma samples

Cited by 71 publications

References 10 publications

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Simultaneous Determination of Metformin and Three Gliptins in Pharmaceutical Formulations Using RP HPLC: Application to Stability Studies on Linagliptin Tablet Formulation

Development of a rapid reversed phase-high performance liquid chromatography method for simultaneous determination of metformin and vildagliptin in formulation and human plasma

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