“…Overall, our computational results have a profound importance for the guidance of the experimental design: not only EF strength, but also EF direction, with respect to the microtubule and kinesin orientation, significantly affect the detachment process. To experimentally verify the predictions, one would ideally need to collect the data from single-molecule imaging techniques, such as TIRF microscopy [60] , but with a high temporal resolution to resolve EF effects within the time step, such as iSCAT [61] , [62] , [63] , [64] . Furthermore, suitable chip technology is required that integrates microfluidics for the sample position and manipulation, electrodes system capable of delivering high EF strengths at sufficiently broad frequency bandwidth (required for ns and shorter electric pulses) and compatibility with the advanced microscopy techniques mentioned above.…”