FAST is a BCL-XL-associated mitochondrial protein

Li, Wei; Kedersha, Nancy; Chen, Samantha; Gilks, Natalie; Lee, Gene; Anderson, Paul S.

doi:10.1016/j.bbrc.2004.03.188

Cited by 25 publications

(36 citation statements)

References 20 publications

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“…The corresponding supernatant is referred to as the S20 fraction. The relative purity of these fractions was previously reported (17).…”

Section: Methodsmentioning

confidence: 99%

“…Cells were maintained in 10% fetal bovine serum (FBS) in Dulbecco's modified Eagle medium (DMEM). The preparation of affinity-purified rabbit polyclonal antisera reactive with FASTN was described previously (17). Antibodies obtained from commercial sources include antihemagglutinin (anti-HA) (murine monoclonal antibody clone 16B12, immunoglobulin G1; Berkeley Antibody Co.), anti-FLAG (murine monoclonal antibody clone; Sigma Chemical Co., St. Louis, Mo.…”

Section: Methodsmentioning

confidence: 99%

“…The construction of expression vectors encoding full-length and mutant FAST were described previously (17). pMT2-HA-TIA1, pMT2-HA-TIA1-RRM, and pMT2-HA-TIA-1-PRD (previously designated TIA-1⌬RRM) were described previously (14).…”

Section: Methodsmentioning

confidence: 99%

“…Tian et al previously described a TIA-1-interacting protein (33) that resides at the outer mitochondrial membrane in association with BCL-X L (17). This Fasactivated serine/threonine phosphoprotein (FAST) is tethered to mitochondria by a lysine/arginine-rich domain at its carboxyl terminus (17). Although FAST is constitutively phosphorylated on serine and threonine residues (33), it is rapidly dephosphorylated in Jurkat cells treated with anti-Fas antibodies.…”

mentioning

confidence: 99%

“…The release of smac/DIABLO promotes apoptosis by inhibiting IAP proteins, a family of E3 ligases that directly and indirectly inhibit the activation of apoptotic caspases (20). Tian et al previously described a TIA-1-interacting protein (33) that resides at the outer mitochondrial membrane in association with BCL-X L (17). This Fasactivated serine/threonine phosphoprotein (FAST) is tethered to mitochondria by a lysine/arginine-rich domain at its carboxyl terminus (17).…”

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confidence: 99%

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FAST Is a Survival Protein That Senses Mitochondrial Stress and Modulates TIA-1-Regulated Changes in Protein Expression

Simarro

Kedersha

et al. 2004

Molecular and Cellular Biology

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“…The corresponding supernatant is referred to as the S20 fraction. The relative purity of these fractions was previously reported (17).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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FAST Is a Survival Protein That Senses Mitochondrial Stress and Modulates TIA-1-Regulated Changes in Protein Expression

Simarro

Kedersha

et al. 2004

Molecular and Cellular Biology

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An extensive study on automated Dewey Decimal Classification

Wang

2009

J. Am. Soc. Inf. Sci.

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In this paper, we present a theoretical analysis and extensive experiments on the automated assignment of Dewey Decimal Classification (DDC) classes to bibliographic data with a supervised machine-learning approach. Library classification systems, such as the DDC, impose great obstacles on state-of-art text categorization (TC) technologies, including deep hierarchy, data sparseness, and skewed distribution. We first analyze statistically the document and category distributions over the DDC, and discuss the obstacles imposed by bibliographic corpora and library classification schemes on TC technology. To overcome these obstacles, we propose an innovative algorithm to reshape the DDC structure into a balanced virtual tree by balancing the category distribution and flattening the hierarchy. To improve the classification effectiveness to a level acceptable to real-world applications, we propose an interactive classification model that is able to predict a class of any depth within a limited number of user interactions. The experiments are conducted on a large bibliographic collection created by the Library of Congress within the science and technology domains over 10 years. With no more than three interactions, a classification accuracy of nearly 90% is achieved, thus providing a practical solution to the automatic bibliographic classification problem.

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Mutations inFASTKD2are associated with mitochondrial disease with multi‐OXPHOS deficiency

Wei

et al. 2020

Human Mutation

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Mutations in FASTKD2, a mitochondrial RNA binding protein, have been associated with mitochondrial encephalomyopathy with isolated complex IV deficiency. However, deficiencies related to other oxidative phosphorylation system (OXPHOS) complexes have not been reported. Here, we identified three novel FASTKD2 mutations, c.808_809insTTTCAGTTTTG, homoplasmic mutation c.868C>T, and heteroplasmic mutation c.1859delT/c.868C>T, in patients with mitochondrial encephalomyopathy.Cell-based complementation assay revealed that these three FASTKD2 mutations were pathogenic. Mitochondrial functional analysis revealed that mutations in FASTKD2 impaired the mitochondrial function in patient-derived lymphocytes due to the deficiency in multi-OXPHOS complexes, whereas mitochondrial complex II remained unaffected. Consistent results were also found in human primary muscle cell and zebrafish with knockdown of FASTKD2. Furthermore, we discovered that FASTKD2 mutation is not inherently associated with epileptic seizures, optic atrophy, and loss of visual function. Alternatively, a patient with FASTKD2 mutation can show sinus tachycardia and hypertrophic cardiomyopathy, which was partially confirmed in zebrafish with knockdown of FASTKD2. In conclusion, both in vivo and in vitro studies suggest that loss of function mutation in FASTKD2 is responsible for multi-OXPHOS complexes deficiency, and FASTKD2-associated mitochondrial disease has a high degree of clinical heterogenicity.FASTKD2, metabolic genetic diseases, mitochondrial disease, OXPHOS complex Xiujuan Wei, Miaomiao Du, and Dongxiao Li contributed equally to this study.

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FAST is a BCL-XL-associated mitochondrial protein

Cited by 25 publications

References 20 publications

FAST Is a Survival Protein That Senses Mitochondrial Stress and Modulates TIA-1-Regulated Changes in Protein Expression

FAST Is a Survival Protein That Senses Mitochondrial Stress and Modulates TIA-1-Regulated Changes in Protein Expression

An extensive study on automated Dewey Decimal Classification

Mutations inFASTKD2are associated with mitochondrial disease with multi‐OXPHOS deficiency

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