Fast imaging strategies for mouse kidney perfusion measurement with pseudocontinuous arterial spin labeling (pCASL) at ultra high magnetic field (11.75 tesla)

Abstract

pCASL RARE demonstrated more advantages for longitudinal preclinical kidney perfusion studies at ultra high magnetic field.

Measurement of Murine Single‐Kidney Glomerular Filtration Rate Using Dynamic Contrast‐Enhanced MRI

“…Although ASL is not a gold standard for perfusion measurement, DCE-MRI notably provided similar renal perfusion values as ASL. In addition, our measured renal perfusion and GFR values in control mice also closely matched those reported in the literature (22,(34)(35)(36). Overall, the good agreement with reference techniques suggests that our DCE-MRI method provides a reliable measurement of murine GFR and renal perfusion.…”

Hyperpolarized 13C magnetic resonance evaluation of renal ischemia reperfusion injury in a murine model

“…The acquisition parameters were as follows: five slice‐selective (4 mm thickness) pre‐saturation pulses (sinc, 2 ms, randomized crusher gradients); non‐selective or slice‐selective inversion (1.1 mm thickness, 180° hypersecant adiabatic full passage pulse, 3.5 ms); inversion time TI = 1.5 ms; fat suppression pulse (sinc, 6 ms); FSE imaging (500 μgauss pulse; echo train length, 32; inter‐TE = 2.8 ms; matrix size, 128 × 128; field of view, 30 × 30 mm 2 ; slice thickness, 2 mm; 30 averages; overall TR = 6 s). The respiration of the mice was kept at a rate in the range 80–100 breaths/min to ensure that the tagging module and the imaging module occurred during the quiescent phase of the respiratory cycle . Perfusion maps were generated in MATLAB (Mathworks Inc., Natick, Massachusetts, USA) as described previously, and expressed in mL/min/100 g of tissue.…”

“…Immediately after the HP 13 C Pyr MR studies, renal perfusion was measured at 14.1 T using a pre-saturated pulsed ASL-FSE sequence with fat suppression and respiratory triggering, as described previously. 29,30 The slice of interest was off-centered by 9 mm in order for the sensitive region of the coil to cover the heart and to allow efficient blood labeling. The acquisition parameters were as follows: 1 five slice-selective (4 mm thickness) pre-saturation pulses (sinc, 2 ms, randomized crusher gradients); 2 non-selective or slice-selective inversion (1.1 mm thickness, 180°hypersecant adiabatic full passage pulse, 3.5 ms); 3 inversion time TI = 1.5 ms; 4 fat suppression pulse (sinc, 6 ms); 5 FSE imaging (500 μgauss pulse; echo train length, 32; inter-TE = 2.8 ms; matrix size, 128 × 128; field of view, 30 × 30 mm 2 ; slice thickness, 2 mm; 30 averages; overall TR = 6 s).…”

Renal Functional MRI and Its Application

“…It has demonstrable advantages in terms of the quantitative perfusion values and SNR and allows an assessment of the volume and heterogeneity of renal masses . Considering various readout strategies, Prevost et al discovered the fact that rapid acquisitions with relaxation enhancement (RARE) and pCASL were more appropriate in ultrahigh magnetic field, in terms of SNR, variation, and image quality . In addition, combining flow‐sensitive alternating inversion recovery (FAIR) with steady‐state free precession (FISP) readout is another option to achieve acquisition with few artifacts in less than 2 seconds.…”